Evaluation of anxiolytic properties of BacoMind extract of plant Bacopa monnieri Linn. in comparison with diazepam in rodent model


  • Rajni Rathore Department of Pharmacology, Atal Bihari Vajpayee Institute of Medical Sciences, Dr Ram Manohar Lohia Hospital and Post Graduate Institute of Medical Education and Research, New Delhi, India
  • Tarun Arora Department of Pharmacology, Lady Hardinge Medical College, New Delhi, India
  • Rashmi Karabasappa Mamadi Department of Pharmacology, St. John’s Medical College and Hospital, Bangalore, Karnataka, India




Anxiolytic, Diazepam, Elevated plus maze, Open field test, BacoMind™


Background: Anxiety is a state characterized by somatic, emotional, cognitive, and behavioral components, associated with significant disability. The pharmacotherapy for anxiety remains limited for achievable safety and tolerability of the medicines. Benzodiazepines use associated with side effects like psychomotor impairment and addiction liability. Due to the ADRs associated with antianxiety drugs, the drug trials have focused on screening herbal medicines that are reportedly used in the treatment of anxiety and which have minimal side effects.

Methods: The anxiolytic activity was examined by using the elevated plus maze (EPM) and open field test (OFT), forty Albino wistar strain rats of both sex of weighing 120 to 200 g were divided into four groups of ten rats each.. Group 1 received vehicle (normal saline); group 2 received diazepam (1 mg/kg); groups 3 and 4 received BacoMind™, 30 and 60 mg/kg oral, respectively.

Results: Rats treated with diazepam (1 mg/kg, p.o.) showed significant (p<0.001) increase in the percentage of open arms entries and time spent whereas, in closed arm the number of entries and time spent were significantly (p<0.05) decreased. Intraperitonial administration of BacoMind™ extract of plant Bacopa monnieri Linn. exhibited significant (p<0.05) increase in the number of open arm entries and time spent with significant (p<0.05) reduction in number of entries and time spent in the closed arm as compared to group 1. BacoMind™ treated rats also produced significant increase in the number of rearings (p<0.05), assisted rearings and number of squares crossed (p<0.01).

Conclusions: BacoMind™ extract of plant Bacopa monnieri Linn possess significant anxiolytic activity in the rats. It can be a promising anxiolytic agent.


Al-Gamal E. Quality of life, anxiety and depression among patients with chronic obstructive pulmonary disease and their spouses. Issues Ment Health Nurs. 2014;35(10):761-7.

Ganguli IH. Epidemiological findings on prevalence of mental disorders in India. Indian J Psychiatr. 2000;42:14-20.

Baxter AJ, Scott KM, Vos T, Whiteford HA. Global prevalence of anxiety disorders: a systematic review and meta-regression. Psychol Med. 2013;43(5):897-910.

Masoumeh E, Mohammad K, Maryam FA. Coriandrum sativum: Evaluation of its anxiolytic effect in the elevated plus-maze. J Ethnopharmacol. 2005;96:365-70.

Russo A, Borrelli F. Bacopa monniera, a reputed nootropic plant: an overview. Phytomedicine. 2005;12(4):305-17.

Tripathi YB, Chaurasia S, Tripathi E, et al. Bacopa monniera Linn. as an antioxidant: mechanism of action. Indian J Exp Biol. 1996;34:523-6.

Prabhakar S, Saraf MK, Pandhi P, Anand A. Bacopa monniera exerts antiamnesic effect on diazepam-induced anterograde amnesia in mice. Psychopharmacology (Berl). 2008;200:27-37.

Goel RK, Sairam K, Babu MD, Tavares IA, Raman A. In vitro evaluation of Bacopa monniera on anti-Helicobacter pylori activity and accumulation of prostaglandins. Phytomedicine. 2003;10:523-7.

Viji V, Shobha B, Kavitha SK, Ratheesh M, Kripa K, Helen A, et al. Betulinic acid isolated from Bacopa monniera (L.) Wettst suppresses lipopolysaccharide stimulated interleukin-6 production through modulation of nuclear factorkappaB in peripheral blood mononuclear cells. Int Immunopharmacol. 2010;10(8):843-9.

Viji V, Kavitha SK, Helen A. Bacopa monniera (L.) wettst inhibits type II collagen-induced arthritis in rats. Phytother Res. 2010;24(9):1377-83.

Chowdhuri DK, Parmar D, Kakkar P, et al. Antistress effects of bacosides of Bacopa monnieri: modulation of Hsp70 expression, superoxide dismutase and cytochrome P450 activity in rat brain. Phytother Res. 2002;16:639-45.

Kishore K, Singh M. Effect of bacosides, alcoholic extract of Bacopa monniera Linn. (brahmi), on experimental amnesia in mice. Indian J Exp Biol. 2005;43:640-5.

Deb DD, Kapoor P, Dighe RP, Padmaja R, Anand MS, D'Souza P, et al. In vitro safety evaluation and anticlastogenic effect of BacoMind on human lymphocytes. Biomed Environ Sci. 2008;21(1):7-23.

Gohil KJ, Patel JA. A review on Bacopa monniera: Current research and future prospects. Int J Green Pharm. 2010;4:1-9.

Bhattacharya SK, Ghosal S. Anxiolytic activity of a standardized extract of Bacopa monniera: an experimental study. Phytomedicine. 1998;5(2):77-82.

Alicia A Walf, Cheryl A Frye.The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nat Protoc. 2007;2(2):322-8.

Nishino T, Takeuchi T, Takechi K, Kamei C. Anxiolytic Effects by Hypnotics. J Pharmacol Sci. 2008;107:349-54.

Rauniar GP, Deo S, Bhattacharya SK. Evaluation of anxiolytic activity of tensarin in mice. Kathmandu Univ Med J (KUMJ). 2007;5:188-94.

Yadav AV, Kawale LA, Nade VS. Anxiolytic effects of Mulberry leaves. Indian J Pharmacol. 2008;40:32-6.

Moser DK. The rust of life, Impact of anxiety on cardiac patients. Am J Crit Care. 2007;16:361-9.

Ghoshal S, Dutta SK. Erythrina chemical and pharmacological evaluation II Alkaloids of Erythina variegata. J Pharm Sci. 1972;61:1274-7.

Lister RG. Ethologically-based animal models of anxiety disorders. Pharmacol Ther 1990;46:321-40.

File SE. Factors controlling measures of anxiety and responses to novelty in the mouse. Behav Brain Res. 2001;125:151-7.

Mechan AO, Moran PM, Elliott M, Young AJ, Joseph MH, Green R. A comparison between dark agouti and Sprague-Dawely rats in their behaviour on the elevated plus-maze, open fi eld apparatus and activity meters and their response to diazepam. Psychopharmacology (Berl). 2002;159:188-95.

Singh HK, Dhawan BN. Effect of bacopa monniera linn. (btim1) extract on Avoidance responses in rat. J Ethnopharmacol. 1982;5:205-14.

Kasture SB, Kasture VS, Joshua AJ, Damodaran A, Amit A. Nootropic activity of BacoMind™, an enriched phytochemical composition from Bacopa monnieri. J Natural Remedies. 2007;7:166-73.




How to Cite

Rathore, R., Arora, T., & Mamadi, R. K. (2019). Evaluation of anxiolytic properties of BacoMind extract of plant Bacopa monnieri Linn. in comparison with diazepam in rodent model. International Journal of Basic & Clinical Pharmacology, 8(11), 2506–2511. https://doi.org/10.18203/2319-2003.ijbcp20194793



Original Research Articles