In vitro evaluation of combination effects of doxorubicin with methylxanthine fractions isolated from Bancha and Pu-erh teas against breast cancer cells


  • Kaloyan D. Georgiev Department of Pharmaceutical Technologies, Faculty of Pharmacy, Prof. Paraskev Stoyanov Medical University, Varna, Bulgaria
  • Iliya J. Slavov Department of Biology, Faculty of Pharmacy, Prof. Paraskev Stoyanov Medical University, Varna, Bulgaria
  • Ivan A. Iliev Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria



Pu-erh, Bancha, Methylxanthine, Breast cancer, Combination effect, Synergism


Background: In the present study we investigated the combination effects of anthracycline antibiotic, doxorubicin, with methylxanthine fractions isolated from Bancha and Pu-erh tea leaves, against MCF-7 and MDA-MB-231 breast cancer cell lines.

Methods: Neutral red uptake assay was used for assessment of cytotoxicity effects and fractional effect analysis and combination index for evaluation of the combination effects.

Results: Doxorubicin was used in varying concentrations by a double dilution method, whereas the methylxanthine fractions were in fixed concentrations – 100, 200, 400 or 600 μg/ml. Results have shown that methylxanthine fraction isolated from Bancha has synergic effects with doxorubicin, while methylxanthines from Pu-erh displayed antagonistic effects.

Conclusions: Тhe obtained results lead us to suspect, that even minor differences in the composition of natural products can lead to significant differences in the biological activity of the product.


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How to Cite

Georgiev, K. D., Slavov, I. J., & Iliev, I. A. (2019). In vitro evaluation of combination effects of doxorubicin with methylxanthine fractions isolated from Bancha and Pu-erh teas against breast cancer cells. International Journal of Basic & Clinical Pharmacology, 8(10), 2167–2171.



Original Research Articles