DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20194266

A study to evaluate the analgesic activity of Origanum vulgare in mice using tail flick method

Siddhi Raveendran, Vikram Rajadnya, Revati Kothari, A. V. Tilak, Sayan Das, Rahul Bhalsinge

Abstract


Background: Pain is a complex experience consisting of physiological and psychological response to a noxious stimulus. Analgesics like opiates and non-steroidal anti-inflammatory drugs are commonly used for relieving pain but are associated with various unwanted side effects; therefore this study was conducted by using Origanum vulgare for their analgesic efficacy.

Methods: In vivo model used was tail flick method. Origanum vulgare (84 mg/kg p.o) was administered in mice. The analgesic activity was studied by recording the reaction time after administration of the drug at frequent intervals up to 3 hours. The results were analysed by ANOVA and Tukey’s test. P-value <0.05 was considered as significant. Pentazocine showed statistically prolongation in the reaction time after 30 min as compared to Origanum vulgare.

Results: In tail flick method, pentazocine showed statistically significant increase in the reaction time after 30 min of administration as compared to control group. However, Origanum vulgare in a dose of 84 mg/kg showed significant increase in the reaction time after 30 min of administration as compared to control group. On comparing pentazocine and Origanum vulgare, pentazocine showed highly significant increase in the reaction time after 30 min as compared to Origanum vulgare at 84 mg/kg dose.

Conclusions: From the present study, it was concluded that extract of Origanum vulgare exerted analgesic activity in both the models. However, it was less potent than pentazocine. Thus, Origanum vulgare can be used in mild to moderate painful conditions.


Keywords


Analgesia, Origanum vulgare, Pentazocine, Tail flick method

Full Text:

PDF

References


Craig AD, Sorkin LS. Pain and analgesia. Available at Encyclopedia of Life Sciences. © 2001 Nature Publishing Group. www.els.net. Accessed 2 October 2008.

Hanson GR, Venturelli PJ, Fleckenstein AE. Drugs and Society. 10th ed. Boston, USA: Jones and Bartlett; 2009.

Vitthalrao AM, Shanbhag T, Kumari MK, BerryKL, Shenoy S. Evaluation of anti-inflammatory and analgesic activities of alcohol extract of Kaempferia galangal in rats. Indian J Physiol Pharmacol. 2011;55(1):13-24.

Duka JA. Handbook of medicinal herbs. Maryland, USA: CRC Press; 2002: 243.

CPCSEA guidelines for laboratory animal facility. Chennai: CPCSEA. Available at: icmr.nic.in/ bioethics/final_cpcsea.pdf. Accessed on 2 October 2008.

Gupta R. Analgesic agents. In: Gupta SK. Drug Screening Method. 3rd ed. New Delhi: Jaypee Brothers Medical Publishers; 2016: 476-497.

Ghosh MN. Fundamentals of experimental pharmacology. 6th ed. Kolkata: Hilton and company; 2015: 46.

Arzi A, Aghel N, Khorasgani ZN, Motahari M. The study of analgesic effect of hydroalcoholic extract of Origanum vulgare in rat by formalin test. Toxicology Letters. 2009;189:105-115.

Khaki MRA, Pahlavan Y, Sepehri G, Sheibani V, Pahlavan B. Antinociceptive effect of aqueous extract of Origanum vulgare L. in male rats: possible involvement of the GABAergic system. Iran J Pharm Res. 2013;12(2):407-13.

Silveira S, Andrade LN, Sousa DP. A review on anti-inflammatory activity of monoterpenes molecules. 2013;18:122754.