Manniophyton fulvum extract attenuates sodium nitroprusside induced oxidative stress in wistar rats
Keywords:SNP, MF, Oxidative stress, Rhodanese, Glutathione
Background: Sodium nitroprusside (SNP) is an antihypertensive agent. It induces toxicity via the release of cyanide ions, nitric oxide (NO) and reactive oxygen species. Manniophyton fulvum (MF) is commonly used in Nigeria due to its therapeutic and nutritional potentials. This study evaluates the phytochemical composition of aqueous extract of MF root and influence in SNP induced oxidative stress in wistar rats.
Methods: Gas chromatography was used for determination of the chemical composition of aqueous extract of MF root. Rat liver homogenate was used for determination of rhodanese, glutathione (GSH) and malondialdehyde (MDA). Twenty (20) adult wistar rats of both sex were randomly divided into 4 different groups comprising 5 animals (n=5). Control (Group A), Groups B, C, D received 2.5 mg/kg body weight of SNP at intervals of 3 hours per day by intraperitoneal injection. In addition, Groups C and D received 200 mg/kg body weight of aqueous root extract of MF and 10 mg/kg body weight of Vitamin E respectively for a period of 7 days.
Results: Flavonoids had the highest composition while allicin had the lowest composition. Diallyl thiosulphinate>methyl allyl thiosulphinate >allyl methyl thiosulphinate. The activity of rhodanese, GSH and MDA concentrations showed that Group B had significant (p<0.05) increase in MDA concentration while GSH showed significant (p<0.05) decrease. Also, the activity of rhodanese showed significant (p<0.05) decrease compared to Group A. However, Groups C and D showed significant increase (p<0.05) in the activity of rhodanese enzyme compared to Group A and Group B. GSH levels of Group C and Group D showed no significant (p>0.05) difference while the MDA concentration showed significant (p<0.05) decrease. Correlation analysis between rhodanese and GSH showed strong significant (p=0.01, r=0.894) positive correlation.
Conclusions: From this study, it can be deduced that the chemical components of aqueous root extract of MF may serve as a pharmacological agent to up regulate detoxification and cytoprotective enzymes. Aqueous root extract of MF can also induced rhodanese to collaborate with GSH and promote inhibition of lipid peroxidation, anti-oxidative reactions and up regulate cyanide detoxification in tissues.
Hottinger DG, Beebe DS, Kozhimannil T, Prielipp RC, Belani KG. Sodium nitroprusside in 2014: A clinical concepts review. J Anaesthesiol Clin Pharmacol. 2014;30(4):462.
Bilska-Wilkosz A, Iciek M, Kowalczyk-Pachel D, Górny M, Sokołowska-Jeżewicz M, Włodek L. Lipoic acid as a possible pharmacological source of hydrogen sulfide/sulfane sulfur. Molecules. 2017;22(3):388.
Spielberg DR, Barrett JS, Hammer GB, Drover DR, Reece T, Cohane CA, et al. Predictors of arterial blood pressure control during deliberate hypotension with sodium nitroprusside in children. Anesthesia Analgesia. 2014;119(4):867.
Dokubo A, Uwakwe AA, Amadi BA. (). A Study on the Antioxidant and anti-inflammatory effects of Aframomum sceptrum and Parinari congensis seed extracts in alloxan-induced diabetic wistar rats. EPRA Int J Res Develop. 2017;(2)7:1-8.
Dokubo A, Odinaka EM. The effect of Aqueous root extract of Manniphytum fulvum (MF) on serum glucose concentration and malondialdehyde (MDA) in wistar albino rats. Int J Innovat Scientific Res. 2016;20:19-24.
Agbaire PO, Emudainohwo JOT, Peretiemo-Clarke BO. Phytochemical screening and toxicity studies on the leaves of Manniophyton fulvum. Int J Plant, Animal Environ Sci. 2013;3(1):1-6.
Anthony OE, Ese AC, Simon OI, Lawrence EO. (). Preliminary phytochemical screening and antidiarrheal properties of Manniophyton fulvum. IOSR J Dent Med Sci. 2013;10:46-52.
Nwabueze JO, Kingsley PIC, Comfort MC. Evaluation of the anti-diabetic properties of Manniophyton fulvum. J Biol Genet Res. 2015;1(8):23-30.
Erhirhie EO, Ekene NE, Ajagbaku DL. Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies. J Nat Sci Res. 2014;4(18):100-6.
Sani M, Sebai H, Refinetti R, Mondal M, Ghanem-Boughanmi N, Boughattas NA, et al. Effects of sodium nitroprusside on mouse erythrocyte catalase activity and malondialdehyde status. Drug Chem Toxicol. 2016;39(3):350-6.
Sorbo BH. Crystalline rhodanese. Enzyme catalyzed reaction. Acta Chemical Scandinavia. 1953;7:1137-45.
Ihm JS, Kim YH. Thiosulfate sulfurtransferase and UDP glucuronosyltransferase activities in cholestatic rat liver induced by common bile duct ligation. Experiment Molecul Med. 1997;29(4):197.
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chemist. 1951;193:265-75.
Ellman GL. Tissue sulphydryl groups. Arch Biochemist Biophysic. 1959;82:70- 7.
Devasagayam TPA, Bloor K K, Ramasarma T. Methods of estimation of lipid peroxidation analysis of merits and demerits. Indian J Biochemist Biophysic. 2003;40:300-8.
Obomanu FG, Dokubo A. Studies on oxidative stress markers of alloxan-induced diabetic wistar rats treated with tetracarpidium conophorum and piper guineense (1: 1 mixture). Int J Advan Res. 2018;6(5):571-9.
Moffett BS, Price JF. Evaluation of sodium nitroprusside toxicity in pediatric cardiac surgical patients. Ann Pharmacotherap. 2008;42(11):1600-4.
Mullens W, Abrahams Z, Francis GS, Skouri HN, Starling RC, Young JB, et al. Sodium nitroprusside for advanced low-output heart failure. J Am Coll Cardiol. 2008;52(3):200-7.
Opasich C, Cioffi G, Gualco A. Nitroprusside in decompensated heart failure: What should a clinician really know. Current Heart Failure Report. 2009;6(3):182-90.
Rhoney D, Peacock WF. Intravenous therapy for hypertensive emergencies, part 1. Am J Health-System Pharm. 2009;66(15):1343-52.
Akomolafe SF, Oboh G, Akindahunsi AA, Akinyemi AJ, Tade OG. Inhibitory effect of aqueous extract of stem bark of Cissus populnea on ferrous sulphate-and sodium nitroprusside-induced oxidative stress in rat’s testes in vitro. ISRN Pharmacol. 2013;2013:130989.
Ojo OA, Oloyede O, Tugbobo O, Olarewaju O, Ojo A. Antioxidant and inhibitory effect of scent leaf (Ocimum gratissimum) on Fe2+ and sodium nitroprusside induced lipid peroxidation in rat brain in vitro. Advan Biol Res. 2014;8(1):8-17.
Quinlan C, Gill D, Waldron M, Awan A. Cyanide poisoning in the post-transplantation patient- a cautionary tale. Pediatr Nephrol. 2008;23(12):2273.
Ibrahim MA, Koorbanally NA, Kiplimo JJ, Islam MS. Anti-oxidative activities of the various extracts of stem bark, root and leaves of Ziziphus mucronata (Rhamnaceae) in vitro. J Med Plant Res. 2012;6(25):4176-84.
Nazari Q A, Mizuno K, Kume T, Takada-Takatori Y, Izumi Y, Akaike A. In vivo brain oxidative stress model induced by microinjection of sodium nitroprusside in mice. J Pharmacol Sci. 2012;120(2):105-11.
Nakajima T. Roles of sulfur metabolism and rhodanese in detoxification and anti-oxidative stress functions in the liver: responses to radiation exposure. Medical science monitor. Int Med J Experiment Clin Res. 2015;21:1721.
Sani M, Sebai H, Ghanem-Boughanmi N, Boughattas NA, Ben-Attia M. Dosing-time dependent oxidative effects of sodium nitroprusside in brain, kidney, and liver of mice. Environ Toxicol Pharmacol. 2014;38(2):625-33.
Bordo D, Bork P. The rhodanese/Cdc25 phosphatase superfamily: sequence–structure–function relations. EMBO Reports. 2002;3(8):741-6.
Nandi DL, Horowitz PM, Westley J. (). Rhodanese as a thioredoxin oxidase. Intl J Biochemistr Cell Biol. 2000;32(4):465-73.
Nagahara N, Katayama A. Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasis. J Biol Chem. 2005;280:34569-77.
Ola-Mudathir FK, Maduagwu EN. Effects of Alium cepa Linn on rhodanese activities and half-life of cyanide in the blood. Intl J Biol Chem Sci. 2015;9(2):1004-12.
Tang T, Sun H, Li Y, Chen P, Liu F. MdRDH1, a HSP67B2-like rhodanese homologue plays a positive role in maintaining redox balance in Musca domestica. Molecul Immunol. 2019;107:115-22.
Selles B, Moseler A, Rouhier N, Couturier J. Rhodanese domain-containing sulfurtransferases: multifaceted proteins involved in sulfur trafficking in plants. J Experim Botan. 2019;70(16):4139-54.
García I, Arenas-Alfonseca L, Moreno I, Gotor C, Romero LC. HCN regulates cellular processes through posttranslational modification of proteins by S-cyanylation. Plant Physiol. 2019;179(1):107-23.