Synergistic effect of metformin and fenugreek on the lipid profile of type-II diabetic patients


  • Manmeet Kaur Department of Pharmacology, Kalpana Chawla Government Medical College, Karnal, Haryana, India
  • Narinder Singh Department of Pharmacology, Sri Guru Ram Das Institute of Medical sciences and Research, Amritsar, Punjab, India



Fenugreek, HDL cholesterol, LDL cholesterol, Metformin, Serum triglycerides, Total cholesterol, Type 2 diabetes mellitus


Background: Fenugreek is a traditional herb which has great relevance in the world since time immemorial due to its multifarious uses. Besides being a rich source of nutrition, it also finds place in the cure of certain pathological conditions. One of its highly beneficial effects is have been observed on the lipid profile of the hyperlipidemic patient. This study was thus undertaken to see the hypolipidemic effect of fenugreek seeds in patients of Type 2 Diabetes Mellitus (DM) as add-on therapy with metformin.

Methods: An open-labelled comparative study of 12 weeks duration was conducted on patients (randomly divided in 2 groups of 30 each) of Type 2 DM. Group 1 was given metformin 500 mg twice a day while group 2 was given 500 mg of metformin along with fenugreek seed powder capsule, 1gm thrice a day. Evaluation for fasting lipid profile estimation was done at the beginning and at the end of the study. Student’s t-test (paired and unpaired) was applied for statistical analysis.

Results: After 12 weeks of treatment, there was significant improvement in the lipid profile of both the groups. However, group 2, that received fenugreek along with metformin showed statistically greater improvement as compared to group 1 which received only metformin.

Conclusions: This study shows the beneficial effects of fenugreek seeds on lipid profile in patients of Type 2 DM and can be used as an add-on therapy with metformin in controlling the lipid profile of Type 2 DM.


Maharani U. Diabetes Mellitus & Hypoglycemia . In: Papadakis MA, McPhee SJ, eds. Current Medical Diagnosis and Treatment. 56th ed. New York: McGraw Hill; 2017:1210-58.

American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. 2004;27(1):5-11.

American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care. 2012;35(1):65-71.

Martha M, Nolte Kennedy MS. Pancreatic hormone and anti-diabetic drugs. In: Katzung BG, Masters SB, Trevor AI, eds. Basic and Clinical Pharmacology. 12th ed. New Delhi: Tata McGraw Hill; 2012:753-67.

Guillausseau PJ, Massin P, Charles MA. Glycemic controland development of retinopathy in type 2 Diabetes Mellitus: a longitudinal study. Diabetes Med. 1998:15(2):151-5.

Davidson MB, Diabetes Mellitus: Diagnosis and Treatment. 21 NewYork, NY. Churchill Livingstone. 2010:795-835.

Management of Diabetes. A national clinical guideline. Edinburgh; Scottish Intercollegiate Guideline Network. 2010 Mar [cited 2013 Nov].Available at: html.

De Fronzo RA. Pharmacological therapy for type 2 diabetes mellitus. Ann Intern Med. 1991;131(4):281-303.

Tripathi KD. Essentials of Medical Pharmacology. 7th ed. New Delhi: Jaypee Brothers Medical Publisher’s; 2013:258-81.

Zhou G, Myers R, Li Y, Chen Y, Shen X, Fenyk-Melody J, et al. Role of AMP-activated protein kinase in mechanism of metformin action. J Clin Investigat. 2001;108(8):1167-74.

Gunton JE, Delhanty PJ, Takahashi SI, Baxter RC. Metformin rapidly increases insulin receptor activation in human liver and signals preferentially through insulin-receptor substrate-2. J Clin Endocrinol Metab. 2003;88(3):1323-32.

Shojaii A, Goushegir A, Dabaghian FH, Abdollahi M, Huseini HF. Herbs and herbal preparations for glycemic control in diabetes mellitus (a systematic review). J Medi Plants Res. 2011;5(16); 3846-55.

Kochhar A, Nagi M. Effect of supplementation of traditional medicinal plants on blood glucose in non-insulin dependent patients: a pilot study. J Med Food. 2005;8(4);545-9.

Basch E, Ulbricht C, Kuo G, Szapary P, Smith, M. Therapeutic applications of fenugreek. Altern Med Rev. 2003;8(1):20-7.

Gupta A, Gupta R, Lal B. Effect of Trigonella foenum-graecum (fenugreek) seeds on glycaemic control and insulin resistance in type 2 diabetes. J Assoc Physicians India. 2001;49:1057-61.

Sharma RD. Hypocholesterolemic activity of fenugreek (Trigonella foenum graecum)-an experimental study in rats. Nutr Rep Int. 1984;(30):221-31.

Tripathi UN, Chandra D. The plant extracts of Momordica charantia and Trigonella foenum graecum have antioxidant and anti-hyperglycemic properties for cardiac tissue during diabetes mellitus. Oxid Med and Cellular Longevity. 2009;2(5):290-6.

Tripathi S, Maurya AK, Kahrana M, Kaul A, Sahu RK. Immunomodulatory property of ethanolic extract of Trigonella foenum-graeceum leaves on mice. Der Pharm Lett. 2012;4(2):708-13.

Tiran D. The use of fenugreek for breast feeding women. Complement Ther Clin Pract. 2003;9(3):155-6.

Sauvaire Y, Petit P, Broca C, Manteghetti M, Baissac Y, Fernandez-Alvarez Jl. Hydroxyleucine, a novel potentiator of insulin secretion. Diabetes. 1998;47(2):206-10.

Kumar VS, Rama Rao DKA, Mohanty S. Comparative study of fenugreek seeds on glycemic index in high and medium dietary fibre containing diets in NIDDM Patients. NJIRM. 2011;2(3):29-37.

Mullaicharam AR , Deori G , Maheswari U. Medicinal Values of Fenugreek-A Review. Res J Pharmaceut Biol Chem Scie. RJPBCS. 2013;4(1):1304-13.

Broca C, Gross R, Petit P Sauvaire Y, Manteghetti M, M Tournier, Masiello P, R Gomis Ribes R 4-Hydro,xyisoleucine: experimental evidence of its insulinotropic and antidiabetic properties. Amer J of Physiol-Endocrinol Metab. 1999;277(4):617-23.

Mohammad S, Taha A, Akhtar K, Bamezai RN, Baquer NZ. In vivo effect of Trigonella foenum graceum on the expression of pyruvate kinase ,phosphoenolpyruvate carboxykinase and distribution of glucose transporter (GLUT4). Can J Physiol Pharmacol. 2006;84:647-54.

Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ. Trigonella foenum graceum(fenugreek)seed powder improves glucose homeostasisin alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem. 2001;224:45-51.

Mohammad S, Taha A, Akhtar K, Bamezai RN, Baquer NZ. Lower doses of vandate in combination with trigonella restore altered carbohydrate metabolosim and antioxidant status in alloxan-diabetic rat. Clin Chem Acta. 2004;342(1-2):105-14.

Madder LZ, Thorn R. Dietery Fibre. Prog Food Nutr Sci. 1987;11(2):153-74.

Baquer NZ, Kumar P, Taha A, Kale RK, Cowsik SM, McLean P. Metabolic and molecular action of Trigonella foenum-graecum (fenugreek) and trace metals in experimental diabetic tissues. J Biosci. 2011;36(2):383-96.

Muraki E, Hayashi Y, Chiba H, Tsunoda N, Kasono K. Dose-dependent effects, safety and tolerability of fenugreek in diet-induced metabolic disorders in rats. Lipids Health Dis. 2011;10(1):240.

Ramadan G, El-Beih NM, Abd El-Kareem HF. Anti-metabolic syndrome and immunostimulant activities of Egyptian fenugreek seeds in diabetic/obese and immunosuppressive rat models. Br J Nutr. 2010;105(7):995-1004.

Vats V, Yadav SP, Grover JK. Effect of T. foenumgraecum on glycogen content of tissues and the key enzymes of carbohydrate metabolism. J Ethnopharmacol. 2003;85(2-3):237-42.

Abbate SL, Brunzell JD. Pathophysiology of hyperlipidemia in diabetes mellitus. J Cardiovasc Pharmacol. 1990;16(9):1-7.

Turner RC, Millns H, Neil HA, Stratton IM, Manley SE, Matthews DR, Holman RR. Risk factors for coronary artery disease in non-insulin dependent diabetes mellitus: United Kingdom Prospective Diabetes Study (UKPDS: 23) BMJ. 1998;316(7134):823-828.

Taskinen MR. Diabetic dyslipidaemia: from basic research to clinical practice. Diabetologia. (2003);46(6):733-49

Kumar K, Kumar S, Datta A, Bandyopadhyay A. Effect of fenugreek seeds on glycemia and dyslipidemia in patients with type 2 diabetes mellitus. Int J Med Sci Public Health. 2015;4(7):997-1000.

Babu K, Yogesh, Raghvendra HL, Kantikar SM, Prakash KB. Antidiabetic and histopathological analysis of fenugreek extract and alloxan induced diabetic rats. Int J Drug Dev& Res. 2010 Apr;2(2):356-64

Kumar A, Kumar B, Kumari S. Diabetes Mellitus and its Herbal Treatment. Int J Biol Med Res. 2014;5(3):4180-5.

Haeri MR, Limaki HK, White CJ, White KN. Non-insulin dependent anti-diabetic activity of (2S, 3R, 4S) 4-hydroxyisoleucine of fenugreek (Trigonella foenum graecum) in streptozotocin-induced type I diabetic rats. Phytomedicine. 2012 May 15;19(7):571-4.

Xue WL, Li XS, Zhang J, Liu YH, Wang ZL, Zhang RJ. Effect of Trigonella foenum-graecum (fenugreek) extract on blood glucose, blood lipid and haemorrogical properties in streptozotocin-induced diabetic rats. Asia Pac J Clin Nutr. 2007;16(1):422-6.

Shrivastava RK, Meena M,Verma M, Kosta S, Kumar R. A prospective study to see the effect of fenugreek seeds powder as a hypoglycemic agent on type 2 Diabetes. Int J Bioassays. 2013;2(10):1344-8.




How to Cite

Kaur, M., & Singh, N. (2019). Synergistic effect of metformin and fenugreek on the lipid profile of type-II diabetic patients. International Journal of Basic & Clinical Pharmacology, 8(8), 1738–1743.



Original Research Articles