Anti-stress activity of Ocimum sanctum and alprazolam in animal models
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20194260Keywords:
Stress, Ocimum sanctum, Alprazolam, Forced swim test, Rotarod testAbstract
Background: Stress is the physiological, psychological and behavioral response by individuals when they perceive a lack of equilibrium between the demands placed upon them and their ability to meet those demands, which over a period of time leads to ill health. There are several ways of coping with stress. Some techniques of time management may help a person to control stress.
Methods: Forced swim test- mice were randomized into two groups according to the body weights. Each group contains six animals. Each individual animal was allowed to swim inside the jar (25-12-25 cm) containing fresh water up to 15 cm height. Mice were allowed swim for 6 min. After initial struggle to escape the animal became immobile. Total immobility period was measured. Rotarod test- mice were randomized into two groups according to body weights. Each group contains six animals. Rats were placed on the lanes. Latency period was recorded at which each rat falls off the rod.
Results: In first experiment, anti-stress activity of Ocimum sanctum in mice was demonstrated by measuring the immobility period during forced swim test and in the second experiment the measurement of the latency period of rats in rotarod apparatus was performed. Both the experimental procedures were compared with standard anti stress drug alprazolam.
Conclusions: The present study suggests that Ocimum sanctum possess significant anti stress activity but less when compared to alprazolam.
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References
Quick JC, Wright TA, Adkins JA, Nelson DL, Quick JD. MD, MPH, FACPM. Preventive stress management in organizations. 2nd ed. Washington, DC: United States: American Psychological Association; 1997.
Bhattacharya SK, Bhattacharya A, Das K, Muruganandam AV, Sairam K. Further investigations on the antioxidant activity of Ocimum sanctum using different paradigms of oxidative stress in rats. J Natural Remed. 2001;1:6-16.
Butterweck V, Wall A, Lieflaender-Wulf U, Winterhoff H, Nahrstedt A. Effects of the total extract and fractions of Hypericum perforatum in animal assays for antidepressant activity. Pharmacopsychiatr. 1997;30:117-24.
Khanna N, Bhatia J. Antinociceptive action of Ocimum sanctum (Tulsi) in mice: possible mechanisms involved. J Ethnopharmacol. 2003;88(2-3):293-6.
Singh S, Nair V, Jain S, Gupta YK. Evaluation of anti-inflammatory activity of plant lipids containing α-linolenicacid. Indian J Exp Biol. 2008;46(6):453-6.
Singh N, Misra N, Srivastava AK, Dixit KS, Gupta GP. Effect of anti-stress plants on biochemical changes during stress reaction. Indian J Pharmacol. 1991;23:137-42.
Bhattacharyya D, Sur TK, Jana U, Debnath PK. Controlled programmed trial of Ocimum sanctum leaf ongeneralized anxiety disorders. Nepal Med Coll J. 2008;10(3):176-9.
Bhargava KP, Singh N. Anti-stress activity of Ocimum sanctum Linn. Indian J Med Res. 1981;73:443-51.
Grover JK, Gupta M, Midha V. Effect of adaptogen Ocimum sanctum on the ascorbic acid and cholesterol contents of heart and adrenals after heat stress in albino rats. Indian J Pharmacol. 1987;19:149.
Imbe H, Iwai-Liao Y, Senba E. Stress induced hyperalgesia: animal model and putative mechanisms. Front Biosci. 2006;11:2179-92.
Liu J, Wang X, Mori A. Immobilisation stress-induced antioxidant defense changes in rat plasma, effect of treatment with reduced glutathione. Int J Biochem. 1994;26:511-7.
Satyavati GV, Gupta AK, Neeraj T. Ocimum sanctum linn. Medicinal Plants of India. 2nd ed. New Delhi, India: ICMR Publication; 1987: 354-371.
Ramachandran NAG, Gunasegaran R. Chemical investigations of certain Indian plants. Indian J Chemistr. 1982;21:979-80.