DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20194255

Investigation of the estrogenic activity of Pueraria candollei variety mirifica extract on rats

Dao Thi Vui, Nguyen Thu Hang, Nguyen Quoc Huy, Bui Thanh Tung

Abstract


Background: Pueraria candollei variety mirifica (PM) has been widely used as ingredient in many rejuvenating products. In this study, we aimed to assess the estrogenic activity of PM extract grown in Vietnam.

Methods: Estrogenic activity of PM extract was estimated on immature female rats by using uterotrophic method to measure the weight of the reproductive organs. Estrogenic activity of PM extract also was investigated in mature female ovariectomized rats by evaluating the vaginal cells growth, reproductive organs weight, serum estradiol concentration.

Results: Our results showed that PM extract at doses of 100 mg/kg, 200 mg/kg had increased the reproductive organs weight in immature rats and female ovariectomized rats. In addition, PM extract had increased the serum estradiol concentration and the vaginal cells growth by increasing the percentage of keratinocytes in female ovariectomized rats.

Conclusions: Our results showed that PM extract has strong estrogenic activity in rats.


Keywords


Estrogenic activity, Pueraria mirifica, Uterotropic assay, Vaginal cytology assay, Ovariectomized rats

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References


Malaivijitnond S. Medical applications of phytoestrogens from the Thai herb Pueraria mirifica. Frontiers Med. 2012;6(1):8-21.

Yusakul G, Putalun W, Udomsin O, Juengwatanatrakul T, Chaichantipyuth C. Comparative analysis of the chemical constituents of two varieties of Pueraria candollei. Fitoterapia. 2011;82(2):203-7.

Cherdshewasart W, Sutjit W, Pulcharoen K, Panriansaen R, Chulasiri M. Antimutagenic potential of the Thai herb, Mucuna collettii Lace. J Ethnopharmacol. 2008;115(1):96-103.

Suthon S, Jaroenporn S, Charoenphandhu N, Suntornsaratoon P, Malaivijitnond S. Anti-osteoporotic effects of Pueraria candollei var. mirifica on bone mineral density and histomorphometry in estrogen-deficient rats. J Nat Med. 2016;70(2):225-33.

Chattuwatthana T, Okello E. Anti-collagenase, anti-elastase and antioxidant activities of Pueraria candollei var. mirifica root extract and coccinia grandis fruit juice extract: an in vitro study. Europ J Med Plant. 2015;5(4):318.

Cherdshewasart W, Kitsamai Y, Malaivijitnond S. Evaluation of the estrogenic activity of the wild Pueraria mirifica by vaginal cornification assay. J Reprod Develop. 2007;53(2):385-93.

Sookvanichsilp N, Soonthornchareonnon N, Boonleang C. Estrogenic activity of the dichloromethane extract from Pueraria mirifica. Fitoterapia, 2008;79(7-8):509-14.

No OT. Uterotrophic bioassay in rodents: a short-term screening test for oestrogenic properties. OECD guidelines for the testing of chemicals. 2007: 4.

Parhizkarsup S, Latiff LA, Rahman SA, Dollah MA, Parichehr H. Assessing estrogenic activity of Nigella sativa in ovariectomized rats using vaginal cornification assay. Afr J Pharm Pharmacol. 2011;5(2):137-42.

Manolagas SC, O'brien CA, Almeida M. The role of estrogen and androgen receptors in bone health and disease. Nature Reviews Endocrinol. 2013;9(12):699.

Uterotrophic Assay. Standard evaluation procedure (SEP). Endocrine Disruptor Screening Program. OCSPP Guideline 890.1600. Washington, DC: 2011; 20460.

De Vos M, Devroey P, Fauser BC. Primary ovarian insufficiency. Lancet. 2010;376(9744):911-21.

Ke HZ, Chen HK, Simmons HA, Qi H, Crawford DT, Pirie CM. Comparative effects of droloxifene, tamoxifen, and estrogen on bone, serum cholesterol, and uterine histology in the ovariectomized rat model. Bone. 1997;20(1):31-9.

Malaivijitnond S, Chansri K, Kijkuokul P, Urasopon N, Cherdshewasart W. Using vaginal cytology to assess the estrogenic activity of phytoestrogen-rich herb. J Ethnopharmacol. 2006;107(3):354-60.

Cherdshewasart W, Sriwatcharakul S, Malaivijitnond S. Variance of estrogenic activity of the phytoestrogen-rich plant. Maturitas. 2008;61(4):350-7.

Cherdshewasart W, Traisup V, Picha P. Determination of the estrogenic activity of wild phytoestrogen-rich Pueraria mirifica by MCF-7 proliferation assay. J Reprod Dev. 2008;54(1):63-7.