DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20193166

Anticonvulsant activity of Adhatoda vasica leaves by pentylenetetrazol model: preliminary evaluation in rats

Sumitha A., Dhanasekaran R., Prathiba A., Geetha M.

Abstract


Background: Epilepsy is a disorder characterised by recurrent seizures of cerebral origin with episodes of sensory, motor phenomenon with or without loss of consciousness. The present study was taken up to evaluate the anticonvulsant effect of aqueous extract of leaves of Adhatoda vasica in rats. Objectives of this study is to evaluate the effect of aqueous extract of Adhatoda vasica leaves on Pentylenetetrazol induced seizures in albino rats and to compare the effect of aqueous extract of Adhatoda vasica leaves with standard dose of sodium valproate on Pentylenetetrazol induced seizures in albino rats.

Methods: Anticonvulsant activity of aqueous extract of Adhatoda vasica was analysed using PTZ (Pentylenetetrazol) model. Groups used were distilled water as control group, Sodium valproate as standard for Pentylenetetrazol and two doses of aqueous extract of Adhatoda vasica (100mg/kg and 200 mg/kg) for this screening model. Parameters observed for PTZ models were abolition of clonic seizures and time duration between injection of PTZ and onset of seizures.

Results: In PTZ model, test group at 200 mg/kg showed 33.33% protection for abolition of clonic seizures, though not comparable to standard group. There was significant increase in the duration of onset of clonic seizures after PTZ injection in both test groups (at 100 mg/kg and 200 mg/kg) when compared to control group.

Conclusions: Aqueous extract of leaves of Adhatoda vasica has shown significant anticonvulsant action in PTZ model.


Keywords


Adhatoda vasica, Aqueous extract, PTZ, Sodium valproate

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References


Satoskar RS, Bhendarkar SD, Rege NN. Drugs effective in Seizure disorders. In: Satoskar RS, Bhendarkar SD, Rege NN, eds. Pharmacology and Pharmacotherapeutics. 23rd ed. Mumbai: Popular Prakashan; 2013: 129-48.

Leonardi MT, Ustan TB. The global burden of Epilepsy. Epilepsia. 2002;43:21-5.

Gourie-Devi M, Gururaj G, Satishchandra P, Subbakrishna DK. Prevalence of neurological disorders in Bangalore, India: a community-based study with a comparison between urban and rural areas. Neuroepidemiol. 2004;23(6):261-8.

Goel D, Agarwal A, Dhanai JS, Semval VD, Mehrotra V, Saxena V, et al. Comprehensive rural epilepsy surveillance programme in Uttarakhand state of India. Neurology India. 2009 May 1;57(3):355-6.

Ganaie SA, Bashir A. Epilepsy in Kashmir, J&K Childhood Seizures & Epilepsy, Types, Causes, Diagnosis, Intervention, Prevalence and Action. Int J Translation Community Dis. 2014;2(2):13-20.

Honavar M, Meldrum BS. Epilepsy. In: Graham DL, Lantos PL, editors. Greenfield’s neuropathology. 7th ed (vol I). London: Arnold; 2002:99-922.

Gerlach AC, Krajewski JL. Antiepileptic drug discovery and development: what have we learned and where are we going. Pharmaceuticals. 2010;3:2884-99.

Butler MS. The role of natural product chemistry in drug discovery. J Nat Prod. 2004;67:2141-53.

Khare CP. Indian medicinal plants,an illustrated dictionary. 2nd ed. New York: Springer;2007:178-9.

Gangwar AK, Ghosh AK. Medicinal uses and Pharmacological activity of Adhatoda vasica. Int J Herbal Med. 2014;2(1):88-91.

Tandon VR, Gupta RK. An Experimental evaluation of anticonvulsant activity of Vitex Negundo. Indian J Physio Pharmacol. 2005;49(2):199-205.

Lone SA, Yadav AS, Sharma AK, Tafazul M, Raghuwanshi DK, Yogeshbadkhane. A review on Adhatoda vasica Nees- An important and high demanded medicinal plant. 2013;3(3):2600-15.

Ganga Raju M, Fernandes J, Vijayanarayana K. Evaluation of antiepileptic activity of the alcoholic extract of Adhatoda vasica leaves in rats. Res J Pharmaceut Biol Chem Sci. 2011;2(3):5-10.

Ambawade SD, Kasture VS, Kasture SB. Anticonvulsant activity of roots and rhizomes of Glycyrrhiza glabra. Ind J Pharmacol. 2002;34(4):251-55.