DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20193174

Hepatoprotective activity of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against CCl4-induced liver injury in rats

Naveen Kumar Bathula, Bimalendu Chowdhury

Abstract


Background: The botanical Latin name of the plant, Turnera aphrodisiaca, describes its ancient use as an aphrodisiac.

Methods: The aim of the present study is to evaluate the protective effect of ethanolic and aqueous extract of Turnera aphrodisiaca leaves against carbon tetrachloride (CCl4)-induced liver damage in male Wistar rats.

Results: Administration with ethanolic and aqueous extract of Turnera aphrodisiaca leaves (200 and 400 mg/kg) for 7 days significantly reduced the impact of CCl4 toxicity on the serum markers of liver damage, aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in a dose dependent matter. In addition, treatment of both the extracts resulted in markedly increased the levels of superoxide dismutase and catalase enzymes in rats. The histopathological studies in the liver of rats also supported that both extracts markedly reduced the toxicity of CCL4 and preserved the histoarchitecture of the liver tissue to near normal.

Conclusion: Thus, the results suggest that ethanolic and aqueous extract of  Turnera aphrodisiaca leaves acts as a potent hepatoprotective agent against CCl4 induced hepatotoxicity in rats.


Keywords


Aqueous extract of Turnera aphrodisiaca leaves ,Carbon tetrachloride, Ethanolic extract of Turnera aphrodisiaca leaves, Hepatoprotective Turnera aphrodisiaca

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References


Ward FM, Daly MJ. Hepatic disease. In: Walker R, Edwards C, editors. Clin. Pharm. Ther. Churchill Livingston: New York; 1999: 195-212.

Pang S, Xin X, Stpierre MV. Determinants of Metabolic Disposition. Rev Pharmacol Toxicol. 1992;32:625-6.

Qadrie ZL, Balasubramanium R, Rehman SU. Screening of liver protective effect of ethanolic leaf extract of Callicarpa lanata (EECL) in ethanol induced hepatotoxicity in wister rats. Int J Basic Clin Pharmacol. 2019;8(3):595-600.

Hocking GM, Thomas CC, editors. A Dictionary of Terms in Pharmacognosy. 1st ed. Spring eld IL: Charles C. Thomas; 1955: 220-25.

Parfitt K, editor. Martindale, the Complete Drug Reference. 32nd ed. London: Pharmaceutical Press; 1999: 531-35.

British Herbal Pharmacopoeia. West Yorks: British Herbal Medicine Association; 1983. Available at: www.phytotherapies.org/monograph-detail.com.

Osol A, Farrar GF, Leuallen EE, Youngken HW, Detweiler DK. Dispensatory of United States of America. 24th ed. Philadelphia: J. B. Lippincott Company; 1947: 522-24.

Caceres A. Medicinal Use Plants in Guatemala. In: Giron L, Caceres A, editors. 1st Ed. Guatemala: Editorial Universataria San Carlos de Guatemala. 1966:160-162.

Saggese D. Medicinal Herbs of Argentina. 10th ed. Rosario, Argentina: Antoghazzi & Co; 1959: 661-63.

Koch L. Drug collection from Bolivia systematically, anatomically and chemically examined. Arch Pharmacol. 1936;274:343-69.

Boericke W. Pocket Manual of Homoeopathic Materia Medica. 9th ed. B. New Delhi: Jain Publisher Private Limited; 1988; 233-35.

Spencer KC, Seigler DS. Tetraphyllin B from Turnera diffusa. Planta Med. 1981;43(2):175-8.

Dominguez XA, Hinojosa M. Mexican medicinal plants. XXVIII. Isolation of 5-hydroxy-7, 3’, 4’-trimethoxy ß avone from Turnera diffusa. Planta Med. 1976;30:68-71.

Auterhoff H, Hackle HP. Components of damiana drug. Arch Pharm. 1968;301:537-44.

Steinmetz EF. Damianae folia. Acta Phytotherapeut 1960;7:1-2.

Parekh J, Karathia N, Chanda S. Antibacterial activity of Bauhinia variegata. J Biomed Res. 2006;9:53-6.

Finar IL. Organic chemistry. Vol 2: Stereochemistry and the chemistry of natural products. 5th edition. Delhi: Pearson Education, 2003;769-771.

Sadasivam S, Manickam A. Biochemical methods. 2nd Ed. New Delhi: New Age International Ltd. 1996;192-193.

Singh K, Khanna AK, Chander R. Hepatoprotective activity of ellagic acid against carbon tetrachloride induced hepatotoxicity in rats. Indian J Expt Biol. 1999;37(10):1025-6.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys. 1984;21(2):130-2.

Sinha AK. Colorimetric assay of catalase. Anal Biochem. 1972;47(2):389-94.

Desmet VJ. Liver tissue examination. J Hepatol. 2003;39(Suppl 1):S43-9.

French SW, Miyamoto K, Ohta Y, Geoffrion Y. Pathogenesis of experimental alcoholic liver disease in the rat. Methods Achiev Exp Pathol. 2000;13:181-207.

Fang HL, Lai JT, Lin WC. Inhibitory effect of olive oil on fibrosis induced by carbon tetrachloride in rat liver. Clin Nutr. 2008;27(6):900-7.

Weber LW, Boll M, Stampfl A. Hepatotoxicity and mechanism of action of haloalkanes: carbon tetrachlorides as a toxicological model. Crit Rev Toxicol. 2003;33(2):105-36.

Ashok SK, Somayaji SN, Bairy AKL. Hepatoprotective effect of Ginkgo biloba against carbon tetrachloride induced hepatic injury in rats. Indian J Pharmacol. 2001;33:260-6.

Bahceioglu IH, Ustundag B, Ozercan I, Ercel E, Bayolas G, Akdere T. Protective effect of Ginkgo biloba extract on CCl4- induced liver damage. Hepatol Res. 1990;15:215-24.

Aleynik IS, Leo AM, Ma X, Aleynik KM, Lieber SC. Polyenyl phosphotidylcholine prevents carbon tetrachloride-induced lipid peroxidation while it attenuates liver fibrosis. J Hepatol. 1997;7:554-61.

Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 3rd Ed. New York: Oxford University Press. 1998:27-30.

Augustyniak A, Wazkilwicz E, Skrzydlewaka E. Preventive action of green tea from changes in the liver antioxidant abilities of different aged rats intoxicated with ethanol. Nutrition 2005;21(9):925-32.

Nakamura Y, Torikai K, Ohigashi H. Toxic dose of a simple phenolic antioxidant, protocatechuic acid, attenuates the glutathione level in ICR mouse liver and kidney. J Agr Food Chem. 2001;49(11):5674-8.

Gabele E, Fron M, Aeteel GE, Uesugi T, Hellerbrand C, Scholmerich J. TNF alpha is required for cholestasis-induced liver fibrosis in the mouse. Biochem Biophys Res Commun. 2009;378(3):348-53.

Masella R, Benedetto RD, Vari R, Filesi C, Govannini C. Novel mechanisms of natural antioxidant compounds in biological systems: involvement of glutathione and glutathione- related enzymes. J Nutr Biochem. 2005;16(10):577-86.

Kim MK, Lee HS, Kim ET, Won NH, Chi YM, Kim BC. Protective effect of aqueous extract of Perilla frutescens on tert-butyl hydroperoxide induced oxidative hepatotoxicity in rats. Food Chem Toxicol. 2007;49(5):1738-44.

Naik SR, Panda VS. Hepatoprotective effect of Ginkgoselect Phytosome in rifampicin induced liver injury in rats: evidence of antioxidant activity. Fitoterapia. 2008;79(6):439-45.

Ree KR, Spector WG. Reversible nature of liver cell damage due to carbon tetrachloride as demonstrated by the use of phenergan. Nature. 1961;190:821-2.

Navarro VJ, Senior JR. Drug-related hepatotoxicity. N Engl J Med. 2006;354(7):731-9.

Rip JW, Rupar CA, Ravi K, Carroll KK. Distribution, metabolism and function of dolichol and polyprenols. Prog Lipid Res. 1985;24:269-309.

Tadeusz J, Teresa J, Krzysztof N. The role of polyprenol in modulation of physical properties of model membranes. Curr Top Biophys. 2001;25:33-8.

Sun F, Hamagawa E, Tsutsui C, Ono Y, Ogiri Y, Kojo S. Evaluation of oxidative stress during apoptosis and necrosis caused by carbon tetrachloride in rat liver. Biochem Biophys Acta. 2001;1535(2):186-91.

Zalatnai A, Sarosi I, Rot A, Lapis K. Inhibitory and promoting effects of carbon tetrachloride- induced liver cirrhosis on the diethylnitrosamine hepatocarcinogenesis in rats. Cancer Lett. 1991;57:67-73.

Mackinnon M, Clayton C, Plummer J, Ahern M, Cmielewski P, Isley A. Iron overload facilitates hepatic fibrosis in the rat alcohol/low-dose carbon tetrachloride model. Hepatol. 1995;21:1083-8.

Natsume M, Tsuji H, Harada A, Akiyama M, Yano T, Ishikura H et al. Attenuated liver fibrosis and depressed serum albumin levels in carbon tetrachloride-treated IL-6- deficient mice. J Leukoc Biol. 1999;66(4):601-8.

Teocharis SE, Margeli AP, Skaltsas SD, Spiliopoulou CA, Koutselinis AS. Induction of metallothionein in the liver of carbon tetrachloride intoxicated rats: an immunohistochemical study. Toxicol. 2001;161:129-38.

Shi J, Aisaka K, Ikawa Y, Wake K. Evidence of hepatocyte apoptosis in rat liver after the administration of carbon tetrachloride. Am J Pathol. 1998;153(2):515-25.