Physico-chemical properties and antibacterial drug performance of amoxicillin from streets in Bamenda, Cameroon

Gerald N. Teke; Christian V. Junior; Stephen L. Tamekou; Penn-Edelqueen W. Nkengla; Jules R. Kuiate

doi:10.18203/2319-2003.ijbcp20192660

Authors

Gerald N. Teke Department of Biomedical Sciences, Faculty of Health Sciences, The University of Bamenda, Bambili, Cameroon
Christian V. Junior Department of Biochemistry, Faculty of Sciences, The University of Bamenda, Bambili, Cameroon
Stephen L. Tamekou Department of Biochemistry, Faculty of Sciences, University of Dschang, Cameroon
Penn-Edelqueen W. Nkengla Department of Biochemistry, Faculty of Sciences, The University of Bamenda, Bambili, Cameroon
Jules R. Kuiate Department of Biochemistry, Faculty of Sciences, University of Dschang, Cameroon

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20192660

Keywords:

Antibacterial performance, Amoxicillin, Bamenda-Cameroon, Physico-chemical properties

Abstract

Background: The advent of microbial drug resistance creates a concern about the quality of anti-infective drugs. The rise in some microbial resistance to therapy has urged us to investigate physico-chemical properties and antibacterial performance of amoxicillin from streets in Bamenda, Cameroon.

Methods: Amoxicillin samples were purchased from street vendors in Bamenda. Cameroon. Visual inspection was done for defects and signs of discoloration, deterioration or other physical disfiguration. Weight uniformity test for each sample of antibiotic tablet was carried out. The time for drug disintegration for each sample of amoxicillin was evaluated. Microbial antibiotic susceptibility tests were done using both the agar disc diffusion and micro well broth dilution methods to check drug performance. Data analysis was done and comparison checked with Student-Newman-Keuls at p<0.05.

Results: The mean weight of amoxicillin drugs ranged from 632.00±11.5 to 748.06±17.9 mg. Tablets from Austria had a significant decrease in weight compared to those from Germany and Nigeria. This was similar with the capsules from India and China. Average disintegration varied significantly from as small as 2.2 to 14.4 minutes. All the amoxicillin samples were active against the tested bacteria with mean zones of inhibitions ranging from 8.33±0.57 to 39.33±0.57 mm. The MICs and MBCs values range from 1 to 64 µg/ml. For the same bacterium the growth inhibitory effects of the various drugs were not all similar.

Conclusions: Some of the amoxicillin samples studied in this paper showed significant differences in their weights and performances (antibacterial activities). Probably the required amount of API was not respected.

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References

Haque M, Nor IR, Zainal Z, Salwani I. Antibiotic prescribing and resistance: knowledge level of medical students of clinical years of University Sultan Zainal Abidin, Malaysia. Ther Clin Risk Manag. 2016;12:413-26.

Ifeyinwa F, Mirza L, Naiela M, Albert VW, Harparkash K. Quality of the Antibiotics- amoxicillin and co-trimoxazole from Ghana, Nigeria, and the United Kingdom. Am. J. Trop. Med. Hyg. 2015;92(6):87-94.

Newton PN, Green MD, Fernandez FM. Impact of poor quality medicines in the ‘developing’ world. Trends Pharmacol Sci. 2010;31:99-101.

Seear M, Gandhi D, Carr R, Dayal A, Raghavan D, Sharma N. The need for better data about counterfeit drugs in developing countries: a proposed standard research methodology tested in Chennai, India. J Clin Pharm Ther. 2011;36:488-95.

Kelesidis T, Kelesidis I, Rafailidis PI, Falagas ME. Counterfeit or substandard antimicrobial drugs: a review of the scientific evidence. J Antimicrob Chemother. 2007;60:214-36.

Al Shami A, Izham M, Abdo Rabbo A. Evaluation of the quality of prescriptions with antibiotics in the government hospitals of Yemen. J Clin Diagn Res. 2011;5:808-12.

Adnyana IK, Murtini S, Roni A, Wardani I. Evaluation of antibacterial activity and determination amoxicillin concentration on generic and branded products. Inte J Phar Pharmace Sci. 2013;5(3):353-57.

Vasco R, Ricardo G, and Hélder V. Anti-competitive impact of pseudo-generics. J Ind Compet Trade. 2014;14(1):83-98.

Kyriacos S, Mroueh M, Chahine RP, Khouzam O. Quality of amoxicillin formulations in some Arab countries. J Clin Pharm Ther. 2008;33:375-79.

Groppo FC, Castro FM, Pacheco AB, Motta RH, Filho TR, Ramacciato JC, et al. Antimicrobial resistance of staphylococcus aureus and oral streptococci strains from high risk endocarditis patients. PubMed. 2005;53(6):410-3.

Habyalimana V, Kindenge JM, Kalenda NT, Dispas A, Yemoa AL, Pierre YS, et al. Analytical tools and strategic approach to detect poor quality medicines, identify unknown components, and timely alerts for appropriate measures: case study of antimalarial medicines. Ame J Ana Chemist. 2015;6:977-94.

Okorie O, Azaka JE, Amadi CM. Pharmaceutical quality assurance of omeprazole capsule brands commonly used in health institutions in southern nigeria. Ame J Pharma Pharmacol. 2016;3(4):33-9.

Soyinka JO, Faleye FJ, Adetogun GE. Quality evaluation of some brands of vitamin C preparations. Nige J Phar Res. 2008;7(1):12-7.

Chen X. Determination of the minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) for a novel antibiotic. Afr J Micro Res. 2011;5(5):572-5.

Vineetha N, Vignesh RA, Sridhar D. Preparation, standardization of antibiotic disc and study of resistance pattern for first-line antibiotics in isolates from clinical samples. international journal of applied research. 2015;1(11):624-31.

Cheesbrough M. District Laboratory Practice in Tropical Countries. 2nd ed. New York, Cambridge University Press; 2006:178-194.

Gerald NT, Betie EN. Antibacterial activity of three cameroonian honey types against some pathogenic species. Int J Biol Chem Sci. 2016;10(6):2477-84.

Teke GN, Kemadjou NE, Kuiate JR. Chemical composition, antimicrobial properties and toxicity evaluation of the essential oil of cupressus lusitanica mill. leaves from Cameroon. BMC Compl Alter Med. 2013;13(1):130.

Chattopadhyay D, Maiti K, Kundu AP, Chakraborty MS, Bhadra R, Mandal SC, et al. Antimicrobial activity of Astonia macrophylla: a folklore of Bay Islands. J Ethnopharmacol. 2001;77(1):49-55.

Hafiz MA, Omair AM, Muhammad BA. Comparative in vitro antibacterial analysis of different brands of cefixime against clinical isolates of Staphylococcus aureus and Escherichia coli. J App Phar Sci. 2012;2(1):109-13.

Gerald NT, Ngolle GE, Akah RT. In vitro antimicrobial activity of some commercial toothpastes. Int J Curr Microbiol App Sci. 2017;6(1):433-46.

Marques Marinho FD, Vianna Soares CD, Carmo VAS, Campos LMM. Quality evaluation of pantoprazole gastro-resistant compounded capsules. Lat Am J Pharm. 2009;28(6):899-906.

Scheshowitsch K, Pereira A, Cruz A, Silva MAS, Stulzer HK. Quality evaluation and dissolution profile of piroxicam manipulated capsules. Lat Am J Pharm. 2007;26(5):645-51.

Vogelpoel H, Welink J, Amidon GL, Junginger HE, Midha KK, Moller H, et al. Biowaiver monographs for immediate release solid oral dosage forms based on biopharmaceutics classification system (BCS) literature data: verapamil hydrochloride, propranolol hydrochloride, and atenolol. J Pharm Sci. 2004;93:1945-56.

Fúlvio GC, Blanca EOM, Paulo CPR. Physicochemical quality evaluation of amoxicillin capsules produced in compounding pharmacies at Diadema, São Paulo, Brazil. J App Pharm Sci. 2015;5(12):029-34.

Abdul Aziz MH, Lipman J, Mouton JW, Hope WW, Roberts JA. Applying pharmacokinetic/pharmacodynamic principles in critically ill patients: optimizing efficacy and reducing resistance development. Seminars in Respiratory and Critical Care Medicine. 2015;36(1):136-53.

William LH, Cormac D, Mycroft HE, Gabriel L. Public health interventions to protect against falsified medicines: a systematic review of internation al, national and local policies. Health Policy and Planning. 2016;31(10):1448-66.

Safo OD, Egbo HA, Nettey H, Konadu DY, Mensah AI. Evaluation of the quality of some antibiotics distributed in Accra and Lagos. Int J Pharm Sci Res. 2016;7(5):1991-2000.