DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20192656

Assessment of antioxidant activity of Ocimum sanctum in healing of thermal burn wound with and without supportive treatment of silver sulfadiazine in rabbits

Vaibhav K. Gupta

Abstract


Background: Burns remain a major public health issue all over the world, especially in developing countries. Pseudomonas aeruginosa is a major cause of infection and death in patients with severe burns.  So, there is a need to develop drugs which will decrease complications and prevent infections more effectively than the presently used drugs.

Methods: Ocimum sanctum, ointment silver sulfadiazine and 24 Rabbits were used in this study. Animals were acclimatized and divided randomly into 04 groups of 06 animals each. After producing partial thickness burn wound ointment silver sulfadiazine was applied daily on the burn wound and Ocimum sanctum was administered orally once daily. Healing was assessed by wound contraction and Oxidative stress and Antioxidant Activity were measured by Malondialdehyde and Superoxide dismutase respectively. Results were analysed by student’s ‘t’ test, one way ANOVA followed by Dennett’s test. P values <0.05 were considered statistically significant.

Results: At the end of treatment (After 28th Day) Group treated with ointment silver sulfadiazine+Ocimum sanctum showed  maximum Percentage of wound contraction (93.17±4.34), maximum decrease of MDA percentage (0.30±0.02 nmol/ml) and maximum increase of SOD percentage (0.0045±0.0002 IU/gm of Hb).

Conclusions: Most effective treatment for burn wound healing in this study was ointment silver sulfadiazine+Ocimum sanctum.


Keywords


Burn Wound healing, Ocimum sanctum, Oxidative stress, Rabbits

Full Text:

PDF

References


Pradhan SL, Pradhan PS. Ayurvedic medicine and anaesthesia. Indian J Anaesth. 2011;55(4):334-9.

Shrivastava P, Durgaprasad S. Burn wound healing property of Cocos nucifera : An Appraisal. Indian J Pharmacol. 2008;40(4):144-6.

Gupta VK, Chandraker S, Keche YN, Ingole N. Preclinical evaluation of burn wound healing property of Ocimum sanctum in rabbits-A histopathological study. Int J Pharm Phytopharmacol Res. 2015;5(3):22-6.

Sutter VL, Hurst V. Sources of pseudomonas aeruginosa infection in burns: study of wound and rectal cultures with phage typing. Ann Surg. 1966;163(4):597-602.

Yoshikawa T, Naito Y. What is oxidative stress? Japan Med Assoc J. 2002;45(7):271-6.

Mühl D, Woth G, Drenkovics L, Varga A, Ghosh S, Csontos C, et al. Comparison of oxidative stress and leukocyte activation in patients with severe sepsis and burn injury. Indian J Med Res. 2011;134(1):69-78.

Sahib AS, Al-Jawad FH, Al-Kaisy AA. Burns, endothelial dysfunction, and oxidative stress: the role of antioxidants. ann burns fire disasters. 2009;22(1):6-11.

Borsuk DE, Gallant M, Richard D, Williams HB. Silver-coated nylon dressings for pediatric burn victims. Can J of Plast Surg. 2007;15(1): 29-31.

Hosseinimehr SJ, Khorasani G, Azadbakht M, Zamani P, Ghasemi M, Ahmadi A. effect of aloe cream versus silver sulfadiazine for healing burn wounds in rats. Acta Dermatovenerol Croat. 2010;18(1):2-7.

Goel A, Kumar S, Singh DK, Bhatia AK. Wound healing potential of Ocimum sanctum Linn. with induction of tumor necrosis factor-α. Indian J Exp Biol. 2010;48(4):402-6.

Pattanayak P, Behera P, Das D, Panda SK. Ocimum sanctum Linn. A reservoir plant for therapeutic applications: An overview. Phcog Rev. 2010;4(7):95-105.

Gupta VK, Pathak SS, Jain MK. Evaluation of burn wound healing property of Ocimum sanctum by monitoring of period of reepithelization in rabbits. Int J Basic Clin Pharmacol. 2016;5(1):146-8.

Gupta VK, Pathak SS. Preclinical evaluation of wound contraction property of Ocimum sanctum with and without supportive treatment of silver sulfadiazine in Rabbits. J Pharmacogn Phytochem. 2016;5(2):96-8.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95(2):351-8.

Marklund S, Marklund G. involvement of superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974;47:469-74.

Tosson Z, Mokadem SA, Attwa E, Nofal I, Sayed MME, Gaber OA. The relation of Malondialdehyde and Superoxide Dismutse to the pathogenesis of Systemic Lupus Erythematosus and Effect of Antioxidants on the disease activity. Egypt Dermat Online J. 2006;2(2):10.

Asha B, Nagabhushan A, Shashikala GH. Study of wound healing activity of topical Ocimum sanctum Linn in albino rats. J Chem Pharm Res. 2011;3(6):122-6.

Devendran G, Balasubramanian U. Qualitative Phytochemical Screening and GC-MS analysis of Ocimum sanctum L. leaves. Asian J Plant Sci Res. 2011;1(4):44-8.