Evaluation of analgesic activity of Toxicodendron radicans in animal model using wistar albino rats

Marzana K. V., Nagapati Prabhakar Bhat, Roopa P. Nayak, Megha Rani N.


Background: Pain forms an integral part of many clinical conditions management is of great importance in every field of medicine. The core of medicine is to preserve and restore patient’s health and to minimize their suffering. Toxicodendron radicans (rhus toxicodendron) is a homoeopathic remedy with anti-inflammatory activity used for various arthritic pain. Rhus toxicodendron is commonly used in skin, mucus membrane affections, pain in joints, tendons, rheumatism in cold season, cellulitis and infection, fever. During the recent exponential rise in the use of alternative medicines and increasing integration into the health service little research has been done on alternative medicine in the context in which it is practiced.

Methods: Wistar albino rats were divided into four groups. group 1 received control (normal saline), group 2 received vehicle (alcohol with distilled water in ratio 1:4), and group 3 received standard (diclofenac-10 mg/kg), group 4 and 5 consist of 2 test groups-rhus toxicodendron 30x and 200c respectively. Analgesic activity was assessed using Hot water tail immersion method and Eddy’s hot plate method. Preliminary pilot study was done with 4 doses rhus toxicodendron 6x, 12x, 30x, 200c respectively. Data was analysed by one-way ANOVA followed by Tukey Kramer multiple comparison test. P value <0.05 was considered as significant.

Results: In Eddy’s hot plate method, rhus toxicodendron 30 showed increase in paw withdrawal time which was statistically very significant at the end of 1 hr compared to the control group whereas statistically rhus toxicodendron 200 showed extremely significant analgesic property. In hot water tail immersion method rhus toxicodendron 30 showed increase in tail withdrawal time at the end of 1hr compared to the control group which was significant statistically.

Conclusions: This study shows that toxicodendron radicans homoeopathic formulations possess significant analgesic property.


Analgesic, Eddy’s hot plate, Hot water tail immersion, Rhus toxicodendron

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Kumar KH, Elavarasi P. Definition of pain and classification of pain disorders. Journal of Advanced Clinical and Research Insights. 2016;3(3):87-90.

Ernst E. Prevalence of use of complementary/alternative medicine: a systematic review. Bulletin of the world Health Organization. 2000;78(2):252-7.

Boericke W. Pocket manual of Homoeopathic Materia Medica and Repertory. New Delhi: B. Jain Publishers: 2007: 489-491.

Parmar NS, Shiv Prakash. Screening methods in pharmacology. Kolkata: Narosa publishing house pvt ltd; 2006:225-235.

Rahul DY, Jain SK, Alok S, Sharma S. Analgesic activity of ethanolic extract of Pongamia pinnata Linn. Leaves. Scholars Research Library. 2011;3(5):180.

Guidelines OECD 425. Acute oral toxicity up and down procedure in OECD guidelines for the testing of chemicals, Organization for Economic Cooperation and Development. 2001;2:12

Sundaram EN, Singh PK, Reddy PK, Kumar S, Nair J, Raven K, Khurana A, et al. Preliminary study to evaluate analgesic and behavioural effects of Lycopodium clavatum in experimental animals. Indian J Res Homoeo. 2013;7(40):168-74.

HG Vogel. Analgesic, anti-inflammatory and anti-pyretic activity. In: drug discovery and evaluation: Pharmacological assays .3rd ed. New York, Springer Verlag; 2008:1013-1014.

S.K Gupta. Drug screening methods.3rd ed. New Delhi: Jaypee brother’s medical publishers; 2015:476-494.

Bikash Medhi, AjayPrakash. Practical manual of experimental and clinical pharmacology. New Delhi: Jaypee brothers medical publishers; 2010:256-259.

N S Parmar, Shiv prakash. Screening methods in pharmacology. Kolkata: Narosa publishing house pvt ltd; 2006:225-235.

D.D Banerjee. Augmented textbook of homoeopathic pharmacy. New Delhi, B. Jain Publishers; 2009:652.

Alam B, Akter F, Parvin N, Pia RS, Akter S, Chowdhury J, et al. Antioxidant, analgesic and anti-inflammatory activities of the methanolic extract of Piper betle leaves. Avicenna J Phytomee. 2013;3(2):112.

Fan SH, Ali NA, Basri DF. Evaluation of analgesic activity of the methanol extract from the galls of quercus infectoria (Olivier) in Rats. Evidence based complementary and alternative medicine. 2014;1-6.

Adeyemi OO, Adeneye AA, Alabi TE. Analgesic activity of the aqueous seed extract of Hunteria Umbellata (K.Schum) hallier F. in rodents. Indian J experiment bio. 2011;49(9):698-703.

Yaksh TL, Woller SA, Ramachandran R, Sorkin LS. The search for novel analgesics: targets and mechanisms. F1000prime reports. 2015;7:56.