Evaluation of antipyretic activity of alcoholic extract of Murraya koenigii leaves in rabbits


  • Naveen Pokala Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India
  • Vijaykumar Sayeli Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India
  • Jayasree T. Department of Pharmacology, Mamata Medical College, Khammam, Telangana, India




Anti pyretic, Digital thermometer, Murraya koenigii, PGE1 induced hyperpyrexia


Background: The present study was carried out to evaluate the antipyretic activity of alcoholic extract of Murraya koenigii leaves in rabbits by using PGE1 induced hyperpyrexia method. We found that not much work has been done on the antipyretic effect of this plant.

Methods: Laboratory breed New Zealand strains of rabbits of either sex weighing 1000-1500 gm were used in the study. The animals were divided into five groups (n=6). The rabbits of all the groups were made febrile by injecting misoprostol (PGE1) subcutaneously in the dose of 100 mcg/kg.  For group 1 and 2, normal saline 2ml/kg as control and aspirin 28mg/kg as standard were given respectively. Alcoholic extract of Murraya koenigii leaves in the doses of 200, 400 and 800mg/kg were administered in remaining three groups respectively. Rectal temperatures were recorded with help of digital thermometer for every 30 min after drug treatment.

Results: Alcoholic extract of Murraya koenigii produced highly significant (p<0.001) antipyretic effect in 400 and 800 mg/kg doses. But onset of action was fast with 800mg/kg dose.

Conclusions: This study concludes that Alcoholic extract of Murraya koenigii has fast onset of action and also exhibited sustained anti pyretic action in New Zealand rabbits.


Wolff SM, Fauci AS, Dale DC. Unusual etiologies of fever and their evaluation. Ann Rev Med. 1975Feb;26(1):277-81.

Satyavati GV, Gupta AK, Tendon N. Medicinal Plants of India. New Delhi: Indian Council of Medical Research. 1987;2:289-299.

Harish K, Anup P, Shruthi SD. A review on Murraya koenigii: multipotential medicinal plant. Asian J Pharm Clin Res. 2012;5(4):5-14.

Jain V, Momin M, Laddha K. Murraya koenigii: An updated review. Int J Ayurved Herb Med. 2012 Aug 19;2(04):607-27.

Gupta P, Nahata A, Dixit V. An update on Murraya koenigii spreng: a multifunctional Ayurvedic herb. Zhong Xi Yi Jie He Xue Bao. 2011;9(8):824-33.

Yadav S, Vats V, Dhunnoo Y, Grover JK. Hypoglycemic and anti hyperglycemic activity of murraya koenigii leaves in diabetic rats. J Ethnopharmacol. 2002;82(2):111-6.

Salwe KJ, Mirunalini R, Mano J, Manimekali K. Evaluation of analgesic activity of Murraya koenigi and Coriandrum sativum leaves extract in animal model. Asian J Pharm Clin Res. 2018;11(1):328-31.

Bikash Medhi. Dose calculation for experimental animals. In: Bikash Medhi, editor. Practical Manual of Experimental and Clinical Pharmacology. 1st Ed. New Delhi: Jaypee Brothers Medical Publishers; 2010:23-25.

Mehta RM. Extraction Processes: Continuous Soxhlet Extraction. In: Mehta RM. Pharmaceutics-1. 3rd Ed. New Delhi:Vallabh Prakashan;2002:157-158.

Van den Heuvel MJ, Dayan AD, Shillaker RO. Evaluation of the BTS approach to the testing of substances and preparations for their acute toxicity. Hum Toxicol. 1987 Jul;6(4):279-91.

Van den Heuvel MJ, Clark DG, Fielder RJ, Koundakjian PP, Oliver GJ, Pelling D, et al. The international validation of a fixed-dose procedure as an alternative to the classical LD50 test. Food Chem Toxicol. 1990 Jan 1;28(7):469-82.

Grover JK. Experiments in Pharmacy and Pharmacology, 1st Ed. Delhi:CBS Publishers;1990:155.

Ranson SW, Kabat H, Magoun HW. Autonomic responses to electrical stimulation of hypothalamus, preoptic region and septum. Archs Neurol Psychiat. 1935;33(3):467-77.

Rang HP, Dale MM, JM Ritter. Anti-Inflammatory and Immuno-Suppressant Drugs: Pharmacology. 4th Ed. Elsevier:Churchill Livingstone, Edinburgh. 1999:229-247.

Rosendorff C, Mooney JJ. Central nervous system sites of action of a purified leucocyte pyrogen. J Physiol. 1971;220(3):597-603.

Milton AS, S Wendlandt. Effects on body temperature of prostaglandins of the A, E and F series on injection into the third ventricle of unanesthetized cats and rabbits. J Physiol. 1971;218(2):325-36.

Patel VR, Patel MG, Patel RK. Anti-pyretic activity of the ethanolic extract of the powdered leaves of Murraya koenigii (L.) Spreng. J Pharm Res. 2009;2(4):731-2.

Rageeb MD, Usman MD, Barhate SD. Phytochemical evaluation and effect of antipyretic activity on Murraya koenigii Spreng. Leaves extract. International J Pharmaceut Chem Sci. 2012;1(1):231-6.

Deepika T, Noorjahan CM. Phytochemical Screening and Thin Layer Chromatographic analysis for Antioxidant activity of Murraya koenigii (Curry leaf). Int J Pharm Life Sci. 2016 Dec 1;7(12):5369-74.

Ramaswamy S, Pillai NP, Gopalkrishnan V, Parmar NS, Ghosh MN. Analgesic effect of O-(Beta hydroxyethyl) rutoside in mice. Indian J Exp Biol. 1985;23(4):219-20.

Hajare SW, Chandra S, Tandan SK, Sarma J, Lal J, Telang AG. Analgesic and antipyretic activities of Dalbergia sissoo leaves. Indian J Pharmacol. 2000 Nov 1;32(6):357-60.

Ray D, Sharatchandra KH, Thokchom IS. Antipyretic, antidiarrhoeal, hypoglycemic and hepatoprotective activities of ethyl acetate extract of Acacia catechu wild in albino rats. Indian J Pharmacol. 2006;38(6):408-13.

Mutalik S, Paridhavi K, Mallikarjuna Rao C, Udupa N. Antipyretic and analgesic effect of Solanum melongena. Indian J Pharmacol. 2003;35(5):312-5.




How to Cite

Pokala, N., Sayeli, V., & T., J. (2019). Evaluation of antipyretic activity of alcoholic extract of Murraya koenigii leaves in rabbits. International Journal of Basic & Clinical Pharmacology, 8(7), 1577–1581. https://doi.org/10.18203/2319-2003.ijbcp20192653



Original Research Articles