DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20161508

Experimental evaluation of the anti-ulcer activity of the ethanolic extract of grape (Vitis vinifera) seed in wistar albino rats against aspirin plus pylorus ligation induced gastric ulcer model

Anand M. Ingale, Venkata Bharat Kumar Pinnelli, Vijaya Rajendran

Abstract


Background: There is an increased demand for newer safer drugs for the treatment of peptic ulcer disease as its incidence is increasing gradually in view of changing lifestyle and stress. The objective of this study was to evaluate the anti-ulcer activity of ethanol extract of seeds of Vitis vinifera.

Methods: The ethanol extract of Vitis vinifera was investigated for its anti-ulcer activity in rats against Aspirin plus Pylorus ligation induced gastric ulcer.

The antiulcer activity was assessed by determining and comparing gastric volume, pH, free and total acidity; ulcer number and its inhibition, ulcer severity and ulcer index.

Results: A significant antiulcer activity was observed. Pylorus ligation model showed significant (p<0.01) reduction in gastric volume, free acidity and ulcer index as compared to control.

Conclusions: This present study indicates that Vitis vinifera seed extract have potential anti-ulcer activity in the model tested.


Keywords


Vitis vinifera, Anti-ulcer, Aspirin, Pylorus ligation, Free acidity, Total acidity

Full Text:

PDF

References


Kumar, Abbas, Fausto, Aster, Robbins, Cotran. Pathological basis of diseases. 8th ed. Pennsylvannia: Elsevier Saunders publishers; 2010.

McPee SI, Papadakis MA. Current medical diagnosis and treatment. NewDelhi: Mc Graw Hill Lange Publications; 2012.

Brunton LL. Goodman and Gilman’s the pharmacological basis of therapeutics. 12th edition. China: McGraw Hill Medical Company; 2012.

Department of pharmacology and therapeutics. herbal medicines: an evidence based look. The University of British Columbia. Canada: The University of British Columbia; 1998.

Grapes. Available at http://www.whfoods.com. Accessed 09 October 2013.

Vaughan JG, Geissler CA. The New Oxford Book of Food Plants (revised and updated edition): New York: Oxford University Press; 1997.

Brown JC, Huang G, Haley-Zitlin V, Jiang X. Antibacterial effects of grape extracts on Helicobacter pylori. Appl Environ Microbiol. 2009;75(3):848-52.

Al-Habib A, Al-Saleh E, Safer A, Afzal M. Bactericidal effect of grape seed extract on Methicillin-resistant Staphylococcus aureus (MRSA). J Toxicol Sci. 2010;35(3):357-64.

Wen-Guang L, Xiao-Yu Z, Xuan T. Anti-inflammatory effect and mechanism of proanthocyanidines from grape seeds. Acta Pharma9col Sin. 2001;22(12):1117-20.

Clifton PM. Effect of grape seed extract and quercition on cardiovascular and endothelial parameters in high-risk subjects. J Biomed Biotechnol. 2004:5:272-8.

Maheswari UM, Rao PGM. Antihepatotoxic effect of grape seed oil in rat. Indian Journal of Pharmacology. 2004;37(3):179-82.

Sreemantula S, Nammi S, Kolanukonda R, Koppula S, Boini KM. Adaptogenic and nootropic activities of aqueous extract of Vitis vinifera (grape seed): an experimental study in rat model. Biomed Central Complement of Alternate Medicine. 2005;5:1.

Vitseva O, Varghese S, Chakrabarti S, Folts JD, Freedman JE. Grape seed and skin extracts inhibit platelet functionand release of reactive oxygen intermediates. J Cardiovasc Pharmacol. 2005;46:445-51.

Roy AM, Baliga MS, Elmets CA, Katiyar SK. Grape seed proanthocyanidins induce apoptosis through, bax, and caspase 3 pathways. Neoplasia. 2005;7(1):24-36.

Kijima I, Phung S, Hur G, Kwok S, Chen S. Grape seed extract is an aromatase inhibitor and a suppressor of aromatase expression. Cancer Res. 2006;66(11):5960-7.

Wen W, Lu J, Zhang K, Chen S. Grape seed extract (GSE) inhibits angiogenesis via suppressing VEGFR signaling pathway. Cancer Prev Res (phila). 2008;17:544-61.

Kim TO, Jeon EJ, Cheung DY, Kim CW, Kim SS, Park S, et al. Gastroprotective effects of grape seed proanthocyanidin extracts against nonsteroid anti-inflammatory drug-induced gastric injury in rats. Gut and Liver. 2013;7(3):282-9.

Cuevas VM, Calzado YR, Guerra YP, Year AO, Despaigne SJ, Ferreiro RM, et al. Effects of grape seed extract, vitamin C, and vitamin E on ethanol- and aspirin-induced ulcers. Advances in Pharmacological Sciences. 2011:1-6.

Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJK. Evaluation of anti-ulcer activity of Polyalthia Longifolia (Sonn.) Thwaites in experimental animals. Indian J Pharmacol. 2008;40(3):126-8.

Firdous SM, Neraja K , Debnath R, Singha D, Sravanthi K. Evaluation of antiulcer activity of ethanolic extract of sechium edule fruits in experimental rats. Int J Pharm Sci. 2012;4(1):374-7.

Bickel M. Activity on gastrointestinal tract. in In: Vogel GH, Vogel WH, Scholkens BA, Sandow J, Muller G. , Vogel W F. Drug discovery and evaluation. Pharmacological assays. 2nd ed. Germany: Springer Publications; 2002:867-872.

Ingale AM, Rajendran V, Pinnelli VB. Experimental evaluation of the anti-ulcer activity of grape (vitis vinifera) seed extract in wistar albino rats. International Journal of Applied Biology and Pharmaceutical Technology. 2014;5(4):107-12.

Mittal R, Bhardwaj RK. Antiulcer agents. In: Gupta SK. Drug Screening Methods. 2nd ed. New Delhi: Jaypee Brothers Medical Publishers (P) ltd.; 2009.

Fine AM. Oligomeric proanthocyanidin complexes: history, structure, and phytopharmaceutical applications. Altern Med Rev. 2000;5(2):144-51.

Shi J, Yu J, Pohorly JE, Kakuda Y. Polyphenolics in grape seeds- biochemistry and functionality. Journal of Medicinal Food. 2003;6(4):291-9.

Ingale AM, Rajendran V, Pinnelli VB. A comparative evaluation of the anti-ulcer activity of the extracts of seed and skin of vitis vinifera (grape) in wistar albino Rats. Intl J Clin Diag Res. 2014;2(2):I.

Saito M, Hosoyama H, Ariga T, Kataoka S, Yamaji N. Antiulcer activity of grape seed extract and procyanidins. Journal of Agricultural and Food Chemistry. 1998;46(4):1460-4.

Sandhar HK, Kumar B, Prasher S, Tiwari P, Salhan M, Sharma PA. Review of phytochemistry and pharmacology of flavonoids. Internationale Pharmaceutica Sciencia. 2011;1(1):25-41.