An experimental study to test the haemostatic effect of Aloe vera


  • Ramesh H. Department of Pharmacology, Karnataka Institute of Medical Science, Hubli, Karnataka, India
  • Jyothi C. H. Department of Pharmacology, JJM Medical College, Davanagere, Karnataka, India



Aloe vera, Albino rats, Bleeding time, Clotting time, Haemostatic effect


Background: Blood clotting is a process which prevents blood loss during injuries. Blood clots even when it is coming out from the blood vessels. Aloe vera is a perennial plant found all over India. It is commonly used in traditional system of medicine for treatment of wound healing, mouth ulcers, constipation, skin wrinkles and diabetes mellitus It is also used as antioxidant and antimicrobial agent. As it is used in traditional system of medicine for wound healing, this study was under taken to test the possible haemostatic effect of aloe vera.

Methods: 12 rats were divided into two groups (control and test) with 6 rats in each group. Determination of Bleeding Time (BT), rat tail was warmed for one minute in water at 40˚C and then dried. A small cut was made in the middle of the tail with a scalpel. In test group, a drop of aloe vera leaf extract was applied on the injured area immediately after making the cut in the middle of the tail, where as in the control group nothing was applied, and BT estimated. Determination of Clotting Time (CT), 12 test tubes were arranged in water bath at 37˚C. Control Group: 0.4 ml of blood was collected from each rat in the control group and added to 6 test tubes kept in the water bath. Test group: For the remaining 6 test tubes 0.1 ml of aloe vera leaf extract was added. 0.4 ml of blood collected from the test group was added to these test tubes. The CT was estimated for both control group and test group.

Results: The results were statistically analyzed by using unpaired t-test. The reduction in BT and CT for test group was statistically highly significant (p<0.001) compared to control group.

Conclusions: In this study aloe vera leaf extract significantly reduced both BT and CT in the test group.


Ryu JK, Petersen MA, Murray SG, Baeten KM, Meyer-Franke A, Chan JP, et al. Blood coagulation protein fibrinogen promotes autoimmunity and demyelination via chemokine release and antigen presentation. Nature communications. 2015 Sep 10;6:8164.

Chapin JC, Hajjar KA. Fibrinolysis and the control of blood coagulation. Blood reviews. 2015;29(1):17-24.

Sui J, Wang BC and Yu ZW. A novel haemostatic model with triple protective functions. Med Hypotheses. 2009;72:186-7.

Kozen BG, Kircher SJ, Henao J, Godinez FS, Johnson AS. Alternative hemostatic dressing: comparison of CELOX. Hem Con and Quik Clot. Acad Emerg Med. 2008;15:74-81.

Wedmore I, McManus JG, Pusateri AE, Holcomb JB. A special report on the chitosan - based hemostatic dressing: experience in current combat operations. J Trauma. 2006;60:655-8.

Subramanian S, Kumar SD, Arulselvan P, Senthilkumar GP, Rao MUS. Evaluation of Anti-ulcerogenic Potential of Aloe vera Leaf Gel Extract Studied in Experimental Rats. J Pharmacol Toxicol. 2007;2:85-97.

Moghaddasi SM, Verma SK. Aloe vera their chemicals composition and applications: A review. Int J Biol Med Res. 2011;2(1):466-71.

Surjushe A, Vasani R, Saple DG. Aloe vera: a short review. Ind J Dermatol. 2008;53(4):163-6.

Kar SK, Bera TK. Phytochemical constituents of aloe vera and their multifunctional properties: A comprehensive review. IJPSR. 2018;9(4):1416-23.

García-Manzano A, González-Llaven J, Lemini C, Rubio-Póo C. Standardization of rat blood clotting tests with reagents used for humans. Proceedings of the Western Pharmacology Society. 2001;44:153-5.

Weremfo A, Adinortey MB, Pappoe ANM. Hemostatic Effect of the stem juice of Musa paradisiaca L. (Musaceae) in guinea pigs. Advances in Biological Research. 2011;5(4):190-2.

Kamal Kishore. Effect of Aloe vera (Aloe barbadensis) on thrombosis in mice. Pharmacologia. 2015;6:347-54.

Mehta JH, Kularni CG, Jadhav ST, Deshpande AM, Bhise SB. Evaluation of Indian herbs for haemostatic activity. IJRPC. 2013;3(4):6-10.

Dapper DV, Achinike PN, Gwotmut MD. The effects of Aloe vera [gel] on clotting time, prothrombin time and plasma fibrinogen concentration in albino Wistar rats. Port Harcou Med J. 2007;2(1):56-60.




How to Cite

H., R., & C. H., J. (2019). An experimental study to test the haemostatic effect of Aloe vera. International Journal of Basic & Clinical Pharmacology, 8(4), 717–719.



Original Research Articles