DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20190994

Evaluation of antihistaminic activity of quercetin by using histamine induced bronchospasm and clonidine induced catalepsy models

Ramesh H., Reshma Nadaf

Abstract


Background: Histamine is an important mediator of allergic reactions. Even though presently available antihistaminics are effective in treatment of allergic reactions, still there is scope for better new drugs. Quercetin has been used as a nutritional supplement and may be beneficial against a variety of diseases. Some of the beneficial effects include anticancer, antitumor, anti-ulcer, anti-allergy and anti-inflammatory effects. As quercetin is used in traditional system of medicine for treatment of allergies, this study was undertaken to evaluate antihistaminic activity of quercetin.

Methods: In histamine induced bronchospasm model, 18 guinea pigs were divided into 3 groups. Control group received normal saline, standard control group received Chlorpheniramine and test group received Quercetin. All drugs were given once daily for 5 days. Preconvulsive dyspnoea was calculated on day 0 and day 5 for all guinea pigs after administration of Histamine aerosol. In clonidine induced catalepsy model, 18 albino mice were divided into 3 groups. Control group, standard control group and test group received normal saline, Chlorpheniramine and Quercetin respectively. One hour after administration of drugs the mice were given clonidine and catalepsy was measured at 30, 60, 90, 120 and 150 min.

Results: In histamine induced bronchospasm model, both chlorpheniramine and quercetin produced significant protection as compared to control group. In clonidine induced catalepsy model the effect of quercetin was comparable to chlorpheniramine.

Conclusions: Quercetin has significant antihistaminic activity. It appears to be due to H1 receptor blockade, contrary to the belief that it inhibits release of histamine from mast cells.


Keywords


Antihistaminic, Clonidine, Chlorpheniramine, Histamine, Quercetin

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References


Kumar D, Bhujbal SS, Deoda RS, Mudgade SC. In-vitro and in-vivo antiasthmatic studies of Ailanthus excelsa Roxb on guinea pigs. J Sci Res. 2010;2(1):196-202.

Saria A, Lundberg JM, Skofitsch G, Lembeck F. Vascular protein leakage in various tissues induced by substance P, capsaicin, bradykinin, serotonin, histamine and by antigen challenge. Naunyn-Schmiedeberg's Arch Pharmacol. 1983;324(3):212-8.

White MV, Slater JE, Kaliner MA. Histamine and asthma. Am Rev Respir Dis. 1987;135:1165-76.

András F, Merétey K. Histamine: an early messenger in inflammatory and immune reactions. Immunol Today. 1992;13(5):154-6.

Durham SR, Lee TH, Cromwell O, Shaw RJ, Merrett TG, Merrett J, et al. Immunologic studies in allergen-induced late-phase asthmatic reactions. J Allergy Clin Immunol. 1984;74(1):49-60.

Lakhanpal P, Rai DK. Quercetin: a versatile flavonoid. Inter J Med Update. 2007;2(2):22-37.

Kempuraj D, Madhappan B, Christodoulou S, Boucher W, Cao J, Papadopoulou N, et al. Flavonols inhibit proinflammatory mediator release, intracellular calcium ion levels and protein kinase C theta phosphorylation in human mast cells. Brit J Pharmacol. 2005;145(7):934-44.

Park HH, Lee S, Son HY, Park SB, Kim MS, Choi EJ, et al. Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Arch Pharmacal Res. 2008;31(10):1303.

Gopumadhavan S, Rafiq M, Venkataranganna MV, Mitra SK. Antihistaminic and antianaphylactic activity of HK-07, an herbal formulation. Ind J Pharmacol. 2005;37(5):300.

Vidhya A, Indira M. Protective effect of quercetin in the regression of ethanol-induced hepatotoxicity. Indi J Pharmaceut Sci. 2009;71(5):527.

Dinesh K, Bhujbal SS, Patil PS, Buge PV. In vitro and in vivo activities of stem bark of methanolic extract of Ailanthus excelsa Roxb. in the management of asthma. IJP. 2010;6(3):284-9.

Dhanalakshmi S, Khaserao SS, Kasture SB. Effect of ethanolic extract of some anti-asthmatic herbs on clonidine and haloperidol-induced catalepsy in mice. OPEM. 2004;4(2):95-9.

Ferre S, Guix T, Prat G, Jane F, Casas M. Is experimental catalepsy properly measured?. Pharmacol Biochem Behavior. 1990;35(4):753-7.

Jung CH, Lee JY, Cho CH, Kim CJ. Anti-asthmatic action of quercetin and rutin in conscious guinea-pigs challenged with aerosolized ovalbumin. Arch Pharmacal Res. 2007;30(12):1599.

Jadhav JH, Balsara JJ, Chandorkar AG. Involvement of histaminergic mechanisms in the cataleptogenic effect of clonidine in mice. J Pharmacy Pharmacol. 1983;35(10):671-3.