DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20191094

Evaluation of antiulcer activity of ethanolic leaf extract of Coccinia grandis in indomethacin induced gastric ulcer model

Barathane Datchanamurthy, Mythireyi D., Dhivyashanthi C. M.

Abstract


Background: Gastric mucosal ulceration is the most common adverse effect with NSAIDS. Antacids, H2 blockers and PPIs are considered novel in treating ulcers but are not devoid of side effects. Hence, there a need for a drug which is effective against NSAID induced ulcers with no side effects. Coccinia grandis plant is traditionally used for the treatment of gastric/peptic ulcers. Hence, this study has been undertaken to scientifically validate the antiulcer activity of Coccinia grandis leaves against indomethacin induced gastric ulcer model.

Methods: Following preparation of the extract, 24 Wistar rats were divided into 4 groups with 6 rats in each group (n=6). Group 1 received 1% CMC, group 2 received 1% CMC +indomethacin 40 mg/kg, group 3 received ethanolic leaf extract of Coccinia grandis 200 mg/kg +indomethacin 40 mg/kg and group 4 received omeprazole (2 mg/kg) +indomethzacin 40 mg/kg for 7 days. Calculation of ulcer score was done using ulcer index and percentage protection.

Results: The ulcer index score (2.12±0.21) and percentage protection (69.71%) was comparable with the standard drug (1.76±0.11, 74.85%) respectively.

Conclusions: The ethanolic leaf extract of Coccinia grandis showed significant antiulcer activity against indomethacin-induced gastric ulcer. Further studies are warranted to elucidate the exact mechanism of antiulcer activity.


Keywords


Coccinia grandis, Ethanolic extract, Gastric ulcer, Indomethacin, Omeprazole, Ulcer index

Full Text:

PDF

References


Hiruma-Lima CA, Calvo TR, Rodrigues CM, Andrade FD, Vilegas W, Brito AR. Antiulcerogenic activity of Alchornea castaneaefolia: effects on somatostatin, gastrin and prostaglandin. J Ethnopharmacol. 2006;104(1-2):215-24.

Kent-Lioyd KC., Debas HT., Peripheral Regulation of Gastric Acid Secretion. Physiology of the Gastrointestinal Tract. Raven Press, New York: 1994; 1126-1185.

Glavin GB, Szabo S. Experimental gastric mucosal injury: laboratory models reveal mechanisms of pathogenesis and new therapeutic strategies. FASEB J. 1992;6(3):825-31.

Longo D, Fauci A, Kasper D, Hauser S, Jameson J, Loscalzo J. Harrison’s Principles of Internal Medicine. 18th ed. Newyork: McGraw-Hill Professional 2012; 2438.

Anoop A, Jegadeesan M. Biochemical studies on the anti-ulcerogenic potential of Hemidesmus indicus R. Br. var. indicus. J Ethnopharmacol. 2003;84(2-3):149-56.

Warrier PK, Nambiar VPK, Ramankutty C. Indian Medicinal Plants. Orient Longman, Madras. 1994;2: 133-137.

Mahanta M, Mukherjee AK. Neutralisation of lethality, myotoxicity and toxic enzymes of Naja kaouthia venom by Mimosa pudica root extracts. J Ethnopharmacol. 2001;75(1):55-60.

Care V. CPCSEA guidelines for laboratory animal facility. Ind J Pharmacol. 2003;35:257-74.

Kunchandy J, Khanna S, Kulkarni SK. Antiulcer effect of Nigella sativa Linn against gastric ulcers in rats. Arch Int Pharmacodyn. 1985;275:123-38.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochem. 1979;95(2):351-8.

Kakkar P, Das B, Viswanathan PN. A modified spectrophotometric assay of superoxide dismutase. Ind J Biochem Biophys. 1984;21:130–2.

Sinha AK. Colorimetric assay of catalase. Analytical Biochem. 1972;47(2):389-94.

Tipple TE, Rogers LK. Methods for the determination of plasma or tissue glutathione levels. In: Developmental Toxicol. Humana Press, Totowa, NJ. 2012:315-324.

Wallace JL, Sharkey KA, Pharmacotherapy of gastric acidity, peptic ulcers, and gastroesophageal reflux disease. In: Nrunton L, Chabner B, Knollman B, eds. Goodman and Gilman’s the pharmacological basis of therapeutics, 12th ed. New Delhi: McGraw-Hill publishers 2011: 1547-1565.

Rainsford K.D. Mechanisms of gastric contrasted with intestinal damage by non-steroidal anti-inflammatory drugs. In: Rainsford K.D., Velo G.P., eds. Side-Effects of Anti-Inflammatory Drugs. Inflammation and Drug Therapy Series, Springer, Dordrecht; 1987: 2.

Goel RK, Bhattacharya SK. Gastroduodenal mucosal defence and mucosal protective agents. Ind J Exp Biol. 1991;29(8):701.

Manoharan P, Shobana J, Upendarrao G, Thangathirupathi A. Anti-ulcer effect of Coccinia grandis (Linn.) on pylorus ligated (albino) rats. Inter J Pharma Res Dev. 2010;2(5).

Girish C, Vineela S, NarasimhaReddy Y, Reddy OV, Rajasekhar KK, Shankarananth V. Evaluation of Antiulcer Activity of Coccinia grandis Leaves. Res J Pharmacol Pharmacodynamics. 2011;3(2):92-5.

Santharam B, Divya V, Thangathirupathi A. Anti-ulcer activity of ethanolic, aqueous and Total aqueous Extracts of Coccinia grandis Linn. voigt in pyloric ligature induced ulcers in albino rats. International Journal of Pharmacy and Pharmaceutical Sciences 2013; 5(4): 104-6.

Tandon R, Khanna RD, Dorababu M, Goel RK. Oxidative stress and antioxidants status in peptic ulcer and gastric carcinoma. Ind J Physiol Pharmacol. 2004;48(1):115-8.