Preliminary phytochemical screening and GC-MS analysis of Cladophora glomerata: green marine algae
Keywords:Cladophora glomerata, GC-MS, Phytochemical analysis, Seaweed
Background: Seaweeds since ages are excellent source of biologically active ingredients. Several Asian countries have a strong tradition of using various seaweeds in herbal medicines preparations. These plants contain various phytochemical constituents having biological activities. Seaweeds are the source of phytochemicals namely agar-agar, carrageenan and algin, which are extensively used in various industries such as food, confectionary, textiles, pharmaceuticals, dairy and paper industries mostly as gelling, stabilizing and thickening agents. They are also used for human consumption, animal feed and as manure in several countries. Several Asian countries are using various seaweeds in traditional medicines.
Methods: In the present study, the shade dried and methanolic extract of Cladophora glomerata, a marine green algae was subjected to preliminary phytochemical and gas chromatography-mass spectrometry analysis(GC-MS) to identify the various bioactive components.
Results: The methanolic extract of Cladophora glomerata revealed the presence of alkaloids, glycosides, flavonoids, saponins, diterpenes and carbohydrates. The GC-MS analysis of the methanolic extract of Cladophora glomerata showed the presence of 42 different compounds. The major compounds were dibutyl phthalate (27.07%), hexadecanoic acid, methyl ester (9.58%), 1,2-benzene-di-carboxylic acid (8.11%), octatriacontyl trifluoroacetate (6.81%), cholesterol (6.66%).Conclusions: Thus, in the present study of Cladophora glomerata, phytochemical and GC-MS analysis provides an important novel information to support further ongoing studies to evaluate structure of bioactive compound and its pharmacological activities.
Cox, S, Abu-Ghannam N, Gupta, S. An assessment of the antioxidant and antimicrobial activity of six species of edible Irish sea weeds. Inter Food Res J. 2010;17:205-220.
Faulkner DJ. Marine natural products. Natural Product Rep. 2000;17(1):7-55.
Whankatte VR, Ambhore JS. Study of phytochemical screening and antioxidant activities of Cladophora glomerata Linn. collected from Raigad Coast of Konkan (MS) India. J Sci Nat. 2016;7:659-63.
Tyagi N, Bohra A. Screening of phytochemicals of fruit plant and antibacterial potential against Pseufomonas aeruginosa. Biochem Cell Arch. 2002;2:21-24.
Wijesekara I, Kim SK. Angiotensin-I-converting enzyme (ACE) inhibitors from marine resources: Prospects in the pharmaceutical industry. Marine Drugs. 2010;8(4):1080-93.
Wijesekara I, Pangestuti R, Kim SK. Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carb Polymers. 2011;84(1):14-21.
Wijesekara I, Yoon NY, Kim SK. Phlorotannins from Ecklonia cava (Phaeophyceae): biological activities and potential health benefits. Biofactors. 2010;36(6):408-14.
Fabrowska J, Leska B, Schroeder G. Freshwater Cladophora glomerata as a new potential cosmetic raw material. Chemik. 2015;69(8):495-7.
Heiba HI, Al-Easa HS, Rizk AF. Fatty acid composition of twelve algae from the coastal zones of Qatar. Plant Foods Human Nutrition. 1997;51(1):27-34.
Elenkov I, Georgieva T, Hadjieva P, Dimitrova-Konaklieva S, Popov S. Terpenoids and sterols in Cladophora vagabunda. Phytochemistry. 1995;38(2):457-9.
Saadatmand S, Khavarinejad R, Nejadsattari T, Soltani S. Antioxidant and antibacterial activities of Cladophora glomerata (L.) Kütz. in Caspian Sea Coast, Iran. Afr J Biotech. 2011;10(39):7684-9.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Inter Pharmaceut Sci. 2011;1(1):98-106.
Mythili T, Ravindhran R. Phytochemical screening and antimicrobial activity of Sesbania sesban(L.) Merr. Asian J Pharm Clin Res. 2012;5(4):179-82.
Thomas ET, Aneesh TP, Thomas DG, Anandan R. GC-MS analysis of phytochemical compounds present in the rhizomes of Nervilia aragoana Gaud. Asian J Pharm Clin Res. 2013;6(3):68-74.
Cody V, Middleton E, Harborne JB, Baretze A. Plant flavonoids. In: Alan R, eds. Biology and Medicine II: Biochemical, Cellular and Medicinal Properties. Liss Inc. New York; 1988: 330.
Omulokoli E, Khan B, Chhabra SC. Antiplasmodial activity of four Kenyan medicinal plants. J Ethnopharmacol. 1997;56(2):133-7.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82.
Srivastava N, Saurav K, Mohanasrinivasan V, Kannabiran K, Singh M. Antibacterial potential of macroalgae collected from the Madappam coast, India. Brit J Pharmacol Toxicol. 2010;1(2):72-6.
Nobori T, Miura K, Wu DJ, Lois A, Takabayashi K, Carson DA. Deletions of the cyclin-dependent kinase-4 inhibitor gene in multiple human cancers. Nature. 1994;368(6473):753.
Stray F. The natural guide to medicinal herbs and plants. Tiger Books International: London; 1998: 12-16.
Okwu DE, Okwu ME. Chemical composition of Spondias mombin Linn plant parts. J Sustain Agric Environ. 2004;6(2):140-7.
Cherian S, Augusti KT. Insulin sparing action of leucopelargonin derivative isolated from Ficus bengalensis Linn. Ind J Exp Biol. 1995;33:608-11.
Khatiwora E, Adsul VB, Kulkarni M, Deshpande NR, Kashalkar RV. Antibacterial activity of dibutyl phthalate: a secondary metabolite isolated from ipomoea carnea stem. J Pharm Res. 2012;5(1):150-2.
Roy RN, Laskar S, Sen SK. Dibutyl phthalate, the bioactive compound produced by Streptomyces albidoflavus 321.2. Microbiol Res. 2006;161(2):121-6.
Krishnamoorthy K, Subramaniam P. Phytochemical profiling of leaf, stem, and tuber parts of Solena amplexicaulis (Lam.) Gandhi using GC-MS. Inter Scholarly Res Notices. 2014;13.
Ravi R, Zulkrnin H, Shaida N, Rozhan NN, Yusoff N, Raihan N, et al. Evaluation of two different solvents for Azolla pinnata extracts on chemical compositions and larvicidal activity against Aedes albopictus (Diptera: Culicidae). J Chem. 2018;7453816.