DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20161451

Evaluation of anti- inflammatory activity of aqueous extract of Mangifera indica leaves in albino rats

A.Sudha Madhuri, Rajalakshmi Mohanvelu, Ramabhimaiah S.

Abstract


Background: Mangifera indica (MI) commonly known as mango belongs to the family anacardiaceae, distributed in rural and semi urban parts of India. According to Ayurveda, various medical properties are attributed to different parts of the mango tree. The purpose of the study was to evaluate anti-inflammatory activity of aqueous extract of Mangifera indica leaves.

Methods: Aqueous extract of Mangifera indica leaves was prepared and tested for anti-inflammatory activity in albino rats weighing 200-250 gm. The in vivo anti-inflammatory activity was evaluated by using Acute (carrageenan induced paw edema) and chronic (Formalin induced paw edema) animal models. Rats were divided into 4 groups, 6 rats in each group. Group I (control) received 0.1 ml 4% gum acacia suspension. Group II and Group III received Mangifera indica extract at doses of 200 mg/kg and 400 mg/kg body weight. Group IV (standard) received diclofenac 10 mg/kg body weight. All the drugs were administered orally. Anti-inflammatory activity was expressed as percentage inhibition of paw edema.

Results: The percentage inhibition in carrageenan induced paw edema with 200 mg/kg and 400 mg/kg Mangifera indica extract was 44.8% and 53% and in formalin induced paw edema was 47.5% and 64.4% respectively. The two doses of Mangifera indica extract showed significant anti-inflammatory action (p<0.001) in both models.

Conclusions: Aqueous extract of Mangifera indica leaves showed significant dose dependent anti-inflammatory activity.


Keywords


Anti-inflammatory activity, Carrageenan, Edema, Formalin, Mangifera indica

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References


Kumar V, Abbas AK, Fausto N. In: Robbins and Cotran Pathological basis of disease. 7th Ed, Philadelphia, Elsevier Saunders. 2004:47-86.

Rang HP, Dale MM, Ritter JM. Anti-inflammatory and immunosuppressant drugs, chapter 14. Flower RJ; Rang and Dale’s Pharmacology, 6th Ed. Elsevier Publication. 2008;226-45.

Scartezzini P, Speroni E. Review on some plants of Indian traditional medicine with antioxidant activity. J Ethnopharmacol. 2000;71:23-43.

Gilles LS. Ethnomedical uses of plants in Nigeria. Benin: University of Benin press. 1992;46.

Islam MR, Mannan MA, Kabir MHB, Islam A, Olival KJ. Analgesic, anti-inflammatory and antimicrobial effects of ethanol extract of mango leaves. J Bangladesh Agril Univ. 2010;8(2):239-44.

Luka CD, Mohammed A. Evaluation of the antidiabetic property of aqueous extract of Mangifera indica leaf on normal and alloxan- induced diabetic rats. J Nat Prod Plant Resour. 2012;2(2):239-43.

Carvalho AC, Guedes MM, Desouza AL, Trevisan MT, Lima AF, Santos FA et al. Gastroprotective effect of mangiferin: A xanthonoid from Mangifera indica against gastric injury induced by ethanol and indomethacin in rodents. Planta Med. 2007;73:1372-6.

Martínez G, Delgado R, Pérez G, Garrido G, Núñez Sellés AJ, León OS. Evaluation of the in-vitro antioxidant activity of Mangifera indica: Extract (vimang) phytother Res. 2004;14:424-7.

Manna SK, Kuo MT, Aggarwal BB. Over expression of gamma-glutamyl cysteine synthatase suppresses tumor necrosis factor-induced apoptosis and activation of nuclear transcription factor-KappaB and activator protein–1. Oncogene. 1999;18(30):4371-82.

Winter CA, Risely EA, Nusa CW. Carrageenan-induced oedema in the hind paw of the rat as an assay for anti-inflammatory drugs, Proc. Soc Exp Biol Med. 1962;111:544-7.

Hosseinzadeh H, Younesi H. Antinoceptive and anti-inflammatory effects of crocus sativas L stigma and petal extracts in mice, BMC Pharmacol. 2002;2:1.

Patel A, Patel T, Macwan C, Patel M, Chauhan K, Patel J. Evaluation of Anti-inflammatory and Analgesic activity of roots of Rubia cordifolia in rats. J Pharm Sci Res. 2010;2(12):809-13.

Hernandez PM, Gallego RR. Evaluation of the anti-inflammatory and analgesic activity of sideritis anariensis Var. Pannosa in mice. J Ethanopharmacol. 2002;81:43-7.

Chawla AS, Singh M, Murthy MS, Guptha MP, Singh H. Anti-inflammatory action of ferulic acid and its ester in carrageenan induced rat paw edema model. Indian J Exp Biol.1987;25:187-9.

Greenwald RA. Animal model for evaluation of arthritic drugs. Methods and findings in Experimental and clinical pharmacology. 1991;13:75-83.

Ahamed Z, Ramabhimaiah S. Anti-inflammatory activity of Aqueous Extract of Carica papaya seeds in Albino Rats. Biomed Pharmacol J. 2012;5;173-7.

Núñez Sellés AJ, Vélez Castro HT, Agüero-Agüero J, González-González J, Naddeo F, De Simone F, et al. Isolation and quantitative analysis of phenolic antioxidants free sugar and polyols mango (Mangifera indica Linn) stem bark aqueous decoction used in Cuba as a nutritional supplement. J Agri Food Chem. 2002;50:762-6.

Khan MA, Nizami SS, Khan MNI, Azeem SW, Ahmed Z. New triterpenes from Mangifera indica. J Nat Prod. 1994;28:124-30.

Doughari JH, Manzara S. Invitro antibacterial activity of crude leaf extract of Mangifera indica linn. African J Microbiol Res. 2008(2):67-72.