Study on the antiemetic property of methanolic stem extract of Swertia chirata using chick emesis model

Authors

  • Pranjal Kr. Medhi Department of Pharmacology, Basic Sciences Building, Assam Medical College and Hospital, Dibrugarh-786002, Assam, India
  • Kalpana Gohain Department of Pharmacology, Basic Sciences Building, Assam Medical College and Hospital, Dibrugarh-786002, Assam, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20190155

Keywords:

Antiemetic, Chick emesis model, Chlorpromazine, Swertia chirata

Abstract

Background: Swertia chirata has been an important herb known for centuries for its various medicinal uses and bitter taste. The stem of the plant is used as a traditional medicine in an array of diseases including the treatment of vomiting. Therefore, the study was undertaken to explore the possible antiemetic property of methanolic extract of its stems by using chick emesis model.

Methods: 25 male chicks of four days old weighing 25 to 35 grams were fed with copper sulfate anhydride at 50mg/kg body weight to induce emesis. The chicks were grouped into 5 with each group bearing 5 chicks (n=5). Group I (control) received 10ml/kg normal saline; group II (standard) received 150mg/kg chlorpromazine; group III (experimental-1), group IV (experimental-2) and group V (experimental-3) received 50, 100 and 150mg/kg respectively of the extract. All doses are given intraperitoneally. Assessment of antiemetic activity was done by calculating the percentage of inhibition of the number of retches in the chicks.

Results: All the three doses of the extract showed antiemetic activity. The dose of 50 mg/kg showed activity comparable to chlorpromazine, while dose of 100 mg/kg and 150mg/kg showed greater activity than chlorpromazine. Highest antiemetic activity (79.26% inhibition) was shown by a dose of 150mg/kg and lowest (42.22% inhibition) by 50mg/kg.

Conclusions: Methanolic extract from the stems of Swertia chirata has excellent anti-emetic property which can be further investigated for development of potential antiemetic medicines.

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References

Greenberger N. Nausea and Vomiting in Adults. www.msdmanuals.com. 2018. Available at: https://www.msdmanuals.com/home/digestive-disorders/symptoms-of-digestive-disorders/nausea-and-vomiting-in-adults. Accessed 22 August 2018.

Kumar V, Van Staden J. A Review of Swertia chirayita (Gentianaceae) as a traditional medicinal Plant. Front Pharmacol. 2016;6:308.

Singh R, Singh P, Agarwal A. Chemical constituents and bio-pharmacological activities of Swertia chirata: A review. NPAIJ. 2012;8(6):238-47.

Tabassum S, Mahmood S, Hanif J, Hina M, Uzair B. An overview of medicinal importance of Swertia chirayita. Int J f App Sci Technol. 2012;2(1):298-304.

Saidu AN, Abubakar AN, Daniel MU, Kabiru AY. Phytochemical Screening and Effects of Methanolic Extract of Azadirachta Indica Leaf in Alloxan Induced Diabetic Rats. IOSR J Pharma Biol Sci. 2014;9(1):16-20.

OECD (Organization for Economic Cooperation and Development) guidelines for testing chemicals. France: OECD publishing; 2006 July 11, Section 4, health effects; test no.425; Acute oral toxicity; Up and down procedure:1-27. Available at: http://iccvam.neihs.nih.gov/SuppDocs/FedDocs/OECD /OECDtg425.pdf. Accessed 22 August 2018.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and Extraction: A Review. Inte Pharmaceut Sci. 2011;1(1):98-106.

Akita Y, Yang Y, Kawai T, Kinoshita K, Koyama K, Takahashi K et al. New assay method for surveying anti-emetic compounds from natural sources. Natural Product Sci. 1998;4(2):72-7.

Bretz F, Westfall P, Hothorn T. Multiple comparisons using R. Boca Raton: Chapman & Hall/CRC. 2011:31.

Reddy R, Kumar B, Bakshi V. Phytochemical screening and antiemetic activity of Lepidagatis cristata root extract. IJRPP. 2014;3(4):269-72.

Bulbul L, Uddin M, Sushanta S, Tanni S, Nipa A, Baul S. Phytochemial investigation and evaluation of antiemetic and anthelmintic activities of Polygonum lapathifolium roots extract. Int J of Pharm Life Sci. 2013;4(5):2632-7.

Nakayama H, Yamakuni H, Higaki M, Ishikawa H, Imazumi K, Matsuo M, et al. Antiemetic Activity of FK1052, a 5-HT3- and 5-HT4-Receptor Antagonist, in Suncus murinus and Ferrets. J Pharmacol Sci. 2005;98:396-403.

Horn CC, Meyers K, Lim A, Dye M, Pak D, Rinaman L, Yates BJ. Delineation of vagal emetic pathways: intragastric copper sulfate-induced emesis and viral tract tracing in musk shrews. Am J Physiol Regul Integr Comp Physiol. 2014 Jan 15;306(5):R341-51.

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Published

2019-01-24

How to Cite

Medhi, P. K., & Gohain, K. (2019). Study on the antiemetic property of methanolic stem extract of Swertia chirata using chick emesis model. International Journal of Basic & Clinical Pharmacology, 8(2), 327–332. https://doi.org/10.18203/2319-2003.ijbcp20190155

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Original Research Articles