Preclinical evaluation of antidepressant activity of aqueous extract of Piper betle leaves in Swiss albino mice
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20185173Keywords:
Aqueous extract, Antidepressant, Leaf, Piper betle, Swiss albino miceAbstract
Background: There is a growing demand for alternative medicines derived from indigenous plants having natural antioxidants and neuroprotective actions for the treatment of many behavioural disorders such as anxiety and depression. This study was designed to screen antidepressant activity of aqueous extract of Piper betle L. leaf (betel leaf) in Swiss albino mice.
Methods: Swiss albino mice of both sexes weighing 25-30grams were used in the present study. Piper betle leaves aqueous extract (PBAE) was administered to the animals at a dose of 100, 200mg/kg body weight orally for 14 days. On the 14th day, after 1 hour of PBAE administration, experiments on force swim test (FST) and tail suspension (TST) were carried out for studying the level of depression. In FST and TST, time of immobility was noted for a period of 5 minutes.
Results: Data was analyzed by one-way ANOVA followed by Tukey Kramer’s multiple comparison test at P = 0.05. The results were represented as Mean±SE. PBAE at a dose of 100mg/kg has shown significant antidepressant activity, as evidenced by decrease in the immobility time in both the screening tests of depression.
Conclusions: Present results demonstrated that PBAE possess potent antidepressant property. The exact mechanism(s) related to the active compound(s) in Piper betle leaf extract have to be elucidated in future studies.
Metrics
References
World Health Organisation. Depression and Other Common Mental Disorders Global Health Estimates. Geneva: World Health Organisation; 2017. Available at: http://www.searo.who.int/india/depression_in_india.pdf. Accessed 14 June 2018.
Rabiei Z, Jahanbazi S, Alibabaei Z, Rafieiani MK. Antidepressant effects of oleuropein in male mice by forced swim test and tail suspension test. World Fam Med Middle East J Fam Med. 2018 Apr;16(4):132-44.
Ponciano LJ, Rosas-Sánchez GU, Domínguez ER, Rodríguez-Landa JF. Advances in the Preclinical Study of Some Flavonoids as Potential Antidepressant Agents. Scientifica. 2008 Feb:1-14.
Shah SK, Garg G, Jhade D, Patel N. Piper betle: phytochemical, pharmacological and nutritional value in health management. Int J Pharmaceut Sci Rev Res. 2016;38:181-9.
Dwivedi V, Tripathi S. Review study on potential activity of Piper betle. J Pharmacogn Phytochem. 2014;3(4):93-8.
Swati B, Madhusudhanan N. Antidepressant activity of ethanolic extract of Piper betle leaves in mice. Curr Res Neurosci. 2012;2(1):11-6.
Gulhane H, Misra AK, Reddy P, Pandey D, Gulhane R, Varma SK. Effects of Piper betle leaves (paan) extract as anti-depressant and anti-anxiety in experimental animals. Mintage J Pharmaceut Med Sci. 2015 Apr-Jun;4(2):12-5.
Evans WC. Trease & Evan’s Pharmacognosy. 16th ed. Edinburg: Saunders Elseveir;2009:121-5.
Sherikar AS, Mahanthesh MC. Evaluation of aqueous and methanolic extract of leaves of Epipremnum aureum for radical scavenging activity by DPPH Method, total phenolic content, reducing capacity assay and FRAP assay. J Pharmacogn Phytochem. 2015;4(4):36-40.
Organisation for Economic Co-operation and Development.Guidance document on acute oral toxicity testing. Paris: Organisation for Economic Co-operation and development;2001. Available at: http://www.oecd.org/officialdocuments/publicdisplaydocumentpdf/?cote=env/jm/mono(2001)4&doclanguage=en. Accessed 21 March 2018.
Ghosh MN. Fundamentals of experimental pharmacology. 6th ed. Kolkata: Hilton & Company;2015:165.
Shastry R, Sharma A, Sayeli V, Dinkar US. Screening of Antidepressant Activity of Punica granatum in Mice. Pharmacogn J. 2017;9(1):27-9.
Vogel HG(Ed.). Drug Discovery and Evaluation:Pharmacological Assays. 3rd ed. Berlin: Spinger-verlag; 2008.p788-93.
Dhingra. D, Sharma. A. Evaluation of antidepressant like activity of Glycyrrhycin in Mice. Indian J Pharmacology, 2005;6:390-4.
Zarghami M, Chabra A, Khalilian A, Asghar Hoseini A. Antidepressant Effect of Asperugo procumbens L. in Comparison with Fluoxetine: a Randomized Double Blind Clinical Trial. Res J Pharmacog. 2018 Jun 1;5(3):15-20.
Xia F, Li C, Li M, Liao Y, Liu X, Si J, et al. Antidepressant activity of an aqueous extract from okra seeds. RSC Adv. 2018;8(57):32814-22.
Dhingra D, Goyal PK. Evidences for the involvement of monoaminergic and GABAergic systems in antidepressant-like activity of Tinospora cordifolia in mice. Indian J Pharmaceut Sci. 2008 Nov;70(6):761-7.