DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20160766

Antinociceptive effects of purified Curcuma longa in mice

Ashish Sharma, Gajendra Prasad Rauniar

Abstract


Background: Since ancient times Curcuma longa is said to have antinociceptive effects in literature.

Methods: It was a quantitative experimental study done in the laboratory setting of the department of Clinical Pharmacology and Therapeutics, BP Koirala Institute of Medical Sciences, Dharan, Nepal (BPKIHS). Hot plate test, Tail flick test and Writhing test were used for evaluating the antinociceptive effects. Animals were divided into five groups of six each, group I as control, group II as standard control whereas groups III, IV and V as test groups (three doses). Control and the three test drug doses were given for 21 days. Data were presented as mean ┬▒Standard Error of Mean. Statistical differences between the test drug and control groups as well as within the test drug groups were calculated using Mann-Whitney U test. A probability level less than 0.05 (p < 0.05) was considered significant.

Results: Significant effects in comparison to vehicle were observed in all the three anti nociceptive test models at 200mg /kg test dose of aqueous extract of purified Curcuma longa (CL). No significant effect as compared to vehicle was observed at 50mg/kg and 100 mg/kg test doses of CL.

Conclusions: This study showed that CL possesses antinociceptive effect. The mechanism(s) and active principle(s) behind the effects of CL could not be established.


Keywords


Curcuma longa, Antinociceptive, Aqueous extract, Soxhlet

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References


Chan EWC. Effects of Different Drying Methods on the Antioxidant Properties of Leaves and Tea of Ginger Species. Food, Chemistry. 2009;113(1):166-72.

Ruby AJ, Kuttan G, Babu KD, Rajasekharan KN, Kuttan R. Anti-tumour an antioxidant activity of natural curcuminoids. Cancer Lett. 1995;94:79-83.

Selvam R, Subramanian L, Gayathri R, Angayarkanni N. The Anti-oxidant Activity of Turmeric (Curcuma longa). J Ethnopharmacol. 1995;47:59-6.

Ohshiro M, Kuroyanag M, Keno A. Structures of sesquiterpenes from Curcuma longa. Phytochemistry. 1990;29:2201-5.

Kapoor LD. Handbook of Ayurvedic Medicinal Plants. Boca Raton, FL: CRC Press. 1990.

Kirtikar KR, Basu BD, Blatter E, Caius JF, Mhaskar KS. Indian Medicinal Plants. Lalit Mohan Basu, Allahabad, India: 1993;2:1182.

Goud VK, Polasa K, Krishnaswamy K. Effect of turmeric on xenobiotic metabolising enzymes. Plant Foods Hum Nutr. 1993;44:87-92.

Gujral ML, Chowdhury N K, Saxena PN. The effect of certain indigenous remedies on the healing of wounds and ulcers. Ind Med Assoc. 1953;22(7):273-6.

Ghatak N, Basu N. Sodium Curcuminate as An Effective Anti-Inflammatory Agent. Ind J Exp Biol. 1972;10:235-6.

Mukhopadhyay A, Basu N, Ghatak N, Gujral PK. Anti-Inflammatory and Imtant Activities of Curcumin Analogues in Rats. Agents Action. 1982:12:508-15.

Sharma SC, Mukhtar H, Sharma SK, Krishna Murti CR. Lipid peroxideformation in experimental inflammation Biochem Pharmacol. 1972;21;1210-4.

Sharma OP. Antioxidant activity of curcumin and related compounds.Biochem Pharmacol. 1976;25:1811-2.

Chandra D, Gupta SS. Anti-inflammatory and anti-arthritic activity of volatile oil of Curcuma longa (Haldi).Indian J Med Res. 1972;60:138-42.

Tripathi RM, GuptaS S, Chandra D. Anti-trypsin and Antihyaluronidase Activity of Volatile Oil of Curcuma longa (Haldi). Indian J Pharmacol. 1973;5:260-61.

Arora RB, Basu N, Kapoor V, Jain AP. Anti-inflammatory studies on Curcuma longa (turmeric).Indian J Med Res. 1971;59(8):1289-95.

Guidelines for care and use of animals in scientific research. Revised Edition: 2000, INSA, New Delhi. Accessed on September. 2012.

Gupta D, Mukul S, Singh AK, Kumar A, Ali Md, Nath A, et al. Effect of Curcuma longa on Ovary of Endosulfan Exposed Mice. IJPBA. 2012;3(3):617-21.

Diehl KH, Hull R, Morton D, Fister PR, Rabemampianina Y, Smith D. A Good Practice Guide to the Administration of Substances and Removal ofBlood, Including Routes and Volumes. J Appl Toxicol. 2001;21(1):15-23.

Woolfe G, MacDonald AD. The evaluation of the analgesic action of pethidine hydrochloride (Dermol). J Pharmacol Exp Ther. 1944;80:300-30.

Aiyelero OM, Ibrahim ZG, Yaro AH. Analgesic and anti-inflammatory properties of the methanol leaf extract of Ficus ingens (Moraceae) in rodents. Nig Journ Pharm Sci. 2009;8(2):79-86.

Bishnoi M, Patil CS, Kumar A, Kulkarni SK. Analgesic activity of acetyl-11 ketobeta-boswellic acid, a 5-lipoxygenase-enzyme inhibitor. Indian J Pharmacol. 2005;37(4):255-6.

Vogel HG, Vogel WH. Radiant heat and hot-plate method. Drug discovery and evaluation. Germany: Springer - Verlag Berlin Heidelberg. 1997;694-7.

Gupta R, Gupta LK, Bhattacharya SK. Naloxone Blocks the Beneficial Effects of Aqueous Extract of Murraya koenigii (L.) Spreng Leaves in Models of Pain.Eur Rev Med Pharmcol Sci. 2013;17:1748-51.

Koster R, Anderson M, De Beer AJ. Acetic acid for analgesic screening. Proceed. 1959;18:412-6.

Wongsrisakul J, Wichitnithad W, Rojsitthisak P, Towiwat P. Antinociceptive Effects of Curcumin Diethyl Disuccinate in Animal Models. J Health Res. 2010;24(4):175-80.

Loux JL, Smith S, Salem H. Comparative Analgesic Testing of Compounds in Mice Using Writhing Techniques. Arzneimittel forschung. 1978;28(9):1644-7.

Bagad AS, Joseph JA, Bhaskaran N, Agarwal A. Comparative Evaluation of Anti-Inflammatory Activity of Curcuminoids, Turmerones, and Aqueous Extract of Curcuma longa. Advances in Pharmacological Sciences. 2013:1-7.

Johns S, Nikhil S, Yaswanth J, Bhaskar A, Amit A, Sudha S. Analgesic property of different extracts of Curcuma longa (Linn.): An Experimental Study in Animals. Journal of Natural Remedies. 2009;9(1):116-20.

Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology, 5th edn. New Delhi:Churchill Livingstone Publication. 2005;562.

Janssen PAJ, Niememegeers CJ, Dony GH. The inhibitory effects of fentanyl and other morphine-like analgesics on the warm water induced tail withdrawal reflex in rats. Arzneimittelforschung. 1963;13:502-7.

Vogel HG, Vogel WH. Pharmacological assays. In Drug discovery and evaluation: Germany: Springer - Verlag Berlin Heidelberg. 1997;370:402-41.

Amico-Roxas M, Caruso A, Trombadore S, Scifo R, Scapagini U. Gangliosides antinociceptive effects in rodents. Arch Int Pharmacodyn Ther. 1984;272(1):103-17.

Raj PP. Pain mechanism. In: Pain medicine: A comprehensive review.1st edn. Missouri: Mosby-Year Book. 1996:2-23.

Fields HL. Analgesic drugs. In: Pain. Day W, editor. 1st ed. USA: Mac-Graw-Hill. 1987:272.

Kim SS, Oh OJ, Min HY, Park EJ, Kim Y, Park HJ. Eugenol suppresses cyclooxygenase-2 expression in lipopolysaccharide-stimulated mouse macrophage RAW264.7 cells. Life Sci. 2003;73(3):337-48.

Murakami Y, Shoji M, Hirata A, Tanaka S, Yokoe I, Fujisawa S. Dehydroisoeugenol, an isoeugenol dimer, inhibits lipopolysaccharidestimulated nuclear factor kappa B activation and cyclooxygenase-2expression in macrophages. Arch Biochem Biophys. 2005;434(2):326-32.