Evaluation of anti-lipoxygenase activity of Cassia fistula linn leaves using in vitro methods

Anju Gopi, Manju Jisho


Background: Numerous plants are claimed to possess anti-inflammatory phytoconstituents in folk medicine, however, one among them is Cassia fistula linn leaves. The tree is 6-9 m high with straight trunk and smooth bark. It is pale green when young and gets rough and dark when old. The leaves are 23-30cm long and have got 4-8 pairs of oblong leaflets. Due to lack of specific scientific reports regarding its use for its anti-lipoxygenase property, this particular plant was selected for this particular study with the aim to bring scientific evidence for its therapeutic use.

Methods: The anti-lipoxygenase study as carried by using 5-lipoxygenase(5-LOX) assay and 12-lipoxygenase(12-LOX) assay. In both the methods, absorbance of various concentrations of the tests and the control solutions were measured at 234nm.

Results: Preliminary phytochemical study showed the presence of flavonoids, glycosides, tannins and phenolics. It was found that both the 5-LOX and 12-LOX were inhibited by the extract with a 50% inhibitory concentration (IC50) of 6.23mg/ml obtained for the 5-LOX assay and an IC50 of 3.22mg/ml attained for the 12-LOX assay.

Conclusions: The methanolic extract of the plant’s leaves showed anti-lipoxygenase activity similar to Indomethacin, thus ensuring that it could be used as an effective anti-inflammatory medicine.


Anti-lipoxygenase, Anti-inflammatory, Methanolic leaf extract, 50% inhibitory concentration

Full Text:



Smyth EM, Burke A, Fitzgerald GA. Lipid-derived autacoids: eicosanoids and platelet-activating factor. In: Brunton LL, Lazo JS, Parker KL, eds. Goodman and Gilman’s The Pharmacological Basis of Therapeutics. 11th Ed. New York, NY: McGra-Hill; 2005:653-670.

Krithikar KR, Basu BD. Indian medicinal plants. 2nd Ed. Dehradun: International book distributors; 1987:856-860.

Guyton AC, Hall JE. Text book of medical physiology. Inflammation: Role of neutrophils and macrophages. 11th Ed. Philadelphia: Elsevier Saunders; 2006:434-437.

Umamahesari M, AsokKumar K, Somasundaram A, Sivashanmugam T, Subhadradevi, Ravi TK. Xanthine oxidase inhibitory activity of some medicinal plants. J of Ethnopharmacol. 2007:8(3)547-51.

Folkerts G, Kloek J, Muijsers RBR, Nijkamp FP. Reactive nitrogen and oxygen species in airway inflammation. Eur J Pharmacol. 2001;7(4):251-62.

Houghton PJ. Traditional plant medicines as a source of new drugs. In: Evans WC eds. Trease and Evans Pharmacognosy. 16th Ed. New York: Elsevier: 2009:62-74.

Khandelal KR. Practical Pharmacognosy: techniques and experiments. 12th Ed. Pune: Nirali Prakashan; 2004:150-156.

Kumaraswamy MV, Satish S. Free radical scavenging activity and lipoxygenase inhibition of Wood for diafructicosakurz and Butelautilis wall. African J Biotechnol. 2007:7(6)312-8.

Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Mungantiar AA, Dikshit, et al. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedarusdeodoro (Roxb). Loud. wood oil. Indian J Expe Biol. 1999; 32(5):258-61.

Kemal C, Louis-Flamberg P, Krupinsky-Oslen R, Shorter AL. Reductive inactivation of soya bean lipoxygenase I by catechols: a possible mechanism for regulation of lipoxygenase activity. Biochemistry. 1987;26(9):7064-72.

Rackova L, Oblozinsky M, Kostalova D, Kettmann V, Bezakova L. Free radical scavenging activity and lipoxygenase inhibition of Mahoniaaquifolium extract and isoquinolin alkaloids. J Inflamma. 2007;4(15):4-15.

Brash AR. Lipoxygenases: Occurrence, functions, catalysis and acquisition of substrate. The J Biol Chemi. 1999;274(10):23679-82.

Yu C, Chen Y, Cline GW, Zhang D, Zong H, Wang Y, et al. Mechanism by which fatty acids inhibit activation of insulin receptor substrate-1 (IRS)- associated phosphatidylinositol 3-kinase activity in muscle. The J Bio Chemi. 2002;277(22):50230-6.

Satoskar RS, Bhandarkar SD, Rege NN. Pharmacology and pharmacotherapeutics. 20th Ed. Mumbai: Popular prakashan private limited; 2007:160-180.

Tripathi KD. Essentials of Medical Pharmacology. 5th Ed. New Delhi: Jaypee Brothers Medical Publishers; 2004:167-184.

Gobianand K, Vivekanandan P, Pradeep K, Mohan CVR, Karthikeyan S. Anti-inflammatory and antipyretic activities of Indian Medicinal Plant, Cassia Fistula Linn in Wistar Albino rats. Int J Pharmacol. 2010;6(5):719-25.

Mohini AP, Manohar JP, Knode A, Pravin DC, Bhoomi P. In vivo and Invitro screening of medicinal plants for their anti-inflammatory activity. J Appl Pharm Scie. 2012;2(6):19-33.