Phenotypic characterization and susceptibility of gram negative bacteria from surgical site infections in a tertiary care hospital


  • Akhilesh P. S. Tomar Department of Microbiology, NSCB Medical College, Jabalpur, Madhya Pradesh, India
  • Anjali Kushwah Department of Pharmacology, Gajra Raja Medical College, Gwalior, Madhya Pradesh, India



Klebsiella pneumoniae, Multi drug resiatant organism, Pseudomonas aeruginosa, Surgical site infections


Background: Gram negative bacteria are the common isolates among the cases of Surgical Site Infections (SSI). Resistant and specially Multi Drug Resistant (MDR) Gram negative isolates are a serious challenge for the treatment to clinicians. Present study was undertaken for phenotypic characterization and susceptibility pattern of Gram negative bacterial isolates from cases of surgical site infections in a tertiary care institute.

Methods: This descriptive cross sectional hospital based study was conducted in a tertiary care teaching hospital over a period of one and half year from January 2012 to June 2013. Centers for disease control and prevention (CDC) SSI case definitions were used to label a case as SSI. Only culture proven cases, out of clinically suspected was included in the study for evaluation.

Results: During the study period a total of 5949 patients were operated and screened for SSI in the wards under surveillance. Out of which 556 were clinically suspected as a case of SSI. With 408 culture proven cases of SSI the rate of SSI in present study was (6.86%). Rates of SSI were more in dirty (22.54%) and contaminated (13.78%) type of wounds. Among Gram negative bacterial isolates (n=343) from SSI, E. coli (45.18%) was the commonest followed by Pseudomonas aeruginosa (16.03%) and Klebsiella pneumoniae (13.42%). Unpleasant trend in antimicrobial resistance observed during study is a serious concern.

Conclusions: The data presented in this study clearly indicate the continuous need of surveillance of SSI. This will clearly help health care personnel in curtailing down the incidences of SSI.


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How to Cite

Tomar, A. P. S., & Kushwah, A. (2018). Phenotypic characterization and susceptibility of gram negative bacteria from surgical site infections in a tertiary care hospital. International Journal of Basic & Clinical Pharmacology, 7(7), 1371–1376.



Original Research Articles