DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20181182

Phytochemical evaluation and pharmacological screening of ethanolic and aqueous leaf extracts of Pupalia lappacea for antihyperlipidemic and antihyperglycemic activities in alloxan induced diabetic Albino wistar rats

Mehnoor Farheen, Chakrapani Ramesh

Abstract


Background: Diabetes is associated with damage to the liver, pancreas and kidney. The damage may vary among patients. This study assessed the hypoglycaemic and antihyperlipidemic activities of leaves ethanolic and aqueous extracts of Pupalia lappaceae in alloxan induced diabetic albino wistar rats.

Methods: There were Fifty four rats divided into nine groups containing six rats each. Group 1 consists of normal rats that were given only normal saline and served as a control group. Group 2 consists of normal rats that were given alloxan monohydrate (150mg/kg b.w). Group 3 consists alloxan induced diabetic rats that were given daily sterile solution. glibenclamide+simvastatin (5mg/kg) Group 4 consists of alloxan induced diabetic rats that were given daily sterile solution, AEPL extract (100mg/kg). Group 5 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract AEPL (200mg/kg). Group 6 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract AEPL (400mg/kg) Group 7 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract EEPL (100mg/kg), Group 8 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract EEPL (200mg/kg) Group 9 consists of alloxan induced diabetic rats that were given daily sterile solution, drug extract EEPL (400mg/kg respectively for 21days by an intragastric tube with free access of food and water.

Results: Chemical composition of the plant was estimated by GCMS technique several biochemical parameters were assessed. Oral administration of the extract resulted in significant reduction in mean values of blood glucose, triglycerides, total cholesterol, cholesterol ratio, LDL, VLDL, accompanied by increase in the mean value of the HDL in diabetic rats and histopathology of liver, pancreas, and kidney showed significant changes.

Conclusions: The effects produced were closely similar to standard antidiabetic and antihyperlipidemic drug. It can thus be concluded that the ethanolic and aqueous extract of Pupalia lappaceae exhibit antihyperlipidemic and antihyperglycemic activities in alloxan induced diabetes in rats.


Keywords


Antidiabetic, Antihyperlipidemic, Alloxan, Pupalia lappaceae

Full Text:

PDF

References


Rao KB, Giri R, Kesavulu MM, Apparao CH. Herbal medicine in the management of diabetes mellitus. Manphar Vaidhya Patrika. 1997;1(4):5.

Oliver-Bever IS. Medicinal plants in tropical West Africa. Cambridge university press; 1986 Jan 23

Kala, Chandra Prakash. Sajwan. Revitalizing Indian systems of herbal medicine by the National Medicinal Plants Board through institutional networking and capacity building. Current Science. 2007;93(6):797-806.

Acharya D, Shrivastava K. Indigenous Herbal Medicines-Tribal Formulation and traditional herbal practices. India: Aavishkar Publishers Distributor; 2008.

Islam MA, Akhtar MA, Islam MR, Hossain MS, Alam MK, Wahed MI, Rahman BM Anisuzzaman AS, Shaheen SM, Ahmed M. Antidiabetic and hypolipidemic effects of different fractions of Catharanthus roseus (Linn.) on normal and streptozotocin-induced diabetic rats. Journal of Scientific Research. 2009 Apr 23;1(2):334-44.

Holman RR, Turner RC, Textbook of Diabetes Blackwell, Oxford; 1991:345-356.

Murthy BK, Nammi S, Kota MK, Rao RK, Rao NK, Annapurna A. Evaluation of hypoglycemic and antihyperglycemic effects of Datura metel (Linn.) seeds in normal and alloxan-induced diabetic rats. Journal of ethnopharmacology. 2004 Mar 31;91(1):95-8.

Islam MA, Akhtar MA, Islam MR, Hossain MS, Alam MK, Wahed MI, et al. Antidiabetic and hypolipidemic effects of different fractions of Catharanthus roseus (Linn.) on normal and streptozotocininduced diabetic rats. Journal of Scientific Research. 2009 Apr 23;1(2):334-44.

Yokozawa T, Ishida A, Cho EJ, Nakagawa T. The effects of Coptidis Rhizoma extract on a hypercholesterolemic animal model. Phytomedicine. 2003 Jan 1;10(1):17-22.

Mahley RW, Bersot TP. Drug therapy for hypercholesterolemia and dyslipidemia. Goodman and Gilman’s the pharmacological basis of therapeutics. 10th Ed. New York: McGraw Hill; 2001:971-1002.

Khandelwal KR. Practical Pharmacognosy Techniques and experiments; 19th Edition, Pune: Nirali Prakashan; 2008:149-160.

No OT. 423: acute oral toxicity-acute toxic class method. OECD guidelines for the testing of chemicals (section 4: health effects). 2001:1-4.