DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20180032

Anti inflammatory effect of rhizome of Curcuma longa. Linn, in Albino rats by the method of Carrageenin induced paw oedema

Jayan Pariyani Savaringal, Lally M. S.

Abstract


Background: Curcuma longa or turmeric is a popular Ayurvedic herb, traditionally used for various inflammatory conditions including rheumatoid arthritis and spondylitis. Turmeric which containing phytochemical ingredient curcumin is used in India for centuries as a topical anti inflammatory agent. Many of the currently used anti inflammatory agents like NSAID’s and glucocorticoids have many undesirable adverse effects, especially when they are used for long period. In the present study, Curcuma longa, a plant belonging to the Zingiberaceae family was chosen for investigating its anti-inflammatory effects.

Methods: The rhizomes of Curcuma longa were collected locally. The extract was prepared by soxlet extraction with 50% ethanol. Albino rats of Wistar strain (170-250grams) obtained from the animal house of medical college Thiruvananthapuram were used. Aspirin was purchased from sigma Labs, Mumbai. Anti-inflammatory effect of the extract was done in rats by the method of Carrageenin induced paw oedema.

Results: The ethanolic extract of Curcuma longa inhibited the development of oedema at the end of 3 hours. The anti-inflammatory activity exhibited by the extract was dose dependent and statistically significant at dose levels of 1000-mg/kg and comparable to that of standard drug used Aspirin.

Conclusions: The present study with extract of Curcuma longa revealed that it has significant anti-inflammatory activity.


Keywords


Aspirin, Anti-inflammatory effect, Curcuma longa, Carrageenin induced paw oedema

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References


Ghatak N, Basu N. Anti-inflammatory activity of Curcumin and Sodium curcuminate. Indian J Exp Biol. 1972;10:235-6.

Chainami Wi N. Safety and anti-inflammatory activity of curcumin, a component of Turmeric. J Alter Complement Med. 2003;9(1):161-8.

Chensue SW, Ward PA. Inflammation. Ivan. Damjanov, James Linder. Editors. Anderson’s Pathology. 10th Ed. Mosby-year book, Inc; 1996;(1):387-410.

Mitchell RN, Cotran RS. Acute and chronic inflammation. In: Kumar V, Cotran RS, Robbins SL, editors. Basic Pathology. 6thed. Bangalore: Prism Books Pvt Ltd; 1997:25-46.

Rang HP, Dale MM, Ritter JM. Pharmacology. 4th Ed. Edinburgh: Churchill Livingstone; 1999:215-216.

Roberts II LJ, Morrow JD. Analgesic- antipyretic and anti-inflammatory agents and drugs employed in treatment of gout. In: Hardman JG, Limbard LE, Gilman AG, editors. Goodman & Gilman’s. The Pharmacological Basis of Therapeutics. 10th Ed. New York: McGraw-Hill; 2001:215-216.

Winter CA, Risley EA, Nuss GW. Carrageenin induced oedema in hind paw of the rat as an assay for anti-inflammatory drugs. Proc Soc Exp Boil Med 1962;111:544-7.

Chauhan O, Godhwani JL, Khanona NK, Pendse VK. Anti-inflammatory activity of Mukhashukti Bhasma. Indian J Exp Biol. 1998;(36):985-89.

Cashin CH, Dawson W, Kitchen EA. The pharmacology of Benoxaprofen (2-[4-chlorophenyl]-α-methyl-5-benzoxazole acetic acid), LRCL 3794, a new compound with anti-inflammatory activity apparently unrelated to inhibition of Prostaglandin synthesis. J Pharm Pharmacol. 1977;29:330-6.

Singh H, Ghosh MN. Modified Plethysmometer for measuring foot volume of unanaesthetised rats. J Pharm Pharmacol. 1968;20:316-7.

Agarwal S, Goel R. Curcumin and its protective and therapeutic uses. National Journal of Physiology, Pharmacy and Pharmacology. 2016;6(1):1-8.

Chauhan O, Godhwani. JL, Khanna. NK, Pendse VK. Anti-inflammatoy activity of Mukthashukti bhasma. Indian J Exp Biol. 1998;36:985-9.

Larsen GL, Henson PM. Mediators of inflammation. Ann Rev Immunol. 1983;1:335-59.

Brooks PM, Day RO. Nonsteroidal anti-inflammatory drugs: differences and similarities. N Engl J Med. 1991;324:1716-25.

Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol. 2000;32:84-6.

Srivastava R, Puri V, Srimal RC, Dhawan BN. Effect of curcumin on platelet aggregation and vascular prostacyclin synthesis. Arzneim Forsch. 1986;36:715-7.

Surh YJ, Chun KS, Cha HH, Han SS, Keum YS, Park KK, et al. Molecular mechanisms underlying chemo preventive activities of anti-inflammatory phytochemicals: down-regulation of COX-2 and iNOS through suppression of NF-kappa B activation. Mutat Res. 2001;480-1,243-68.

Srivastava R. Inhibition of neutrophil response by curcumin. Agents Actions. 1989;28;298-303.

Dixit M, Rastogi L, Shukla R, Srimal RC. Prevention of ischemia-induced biochemical changes by curcumin and quinidine in the cat heart. Indian J Med Res. 1995;101:31-5.

Chun KS, Keum YS, Han SS, Song YS, Kim SH, Surh YJ. Curcumin inhibits phorbol ester-induced expression of cyclooxygenase-2 in mouse skin through suppression of extracellular signal-regulated kinase. Carcinogenesis. 2003;24(9):1515-24.

Rafathullah S, Tariq M, Al-Yahya MA, Mossa JS, Ageel AM. Evaluation of Curcuma longa for gastric and duodenal ant-ulcer activity in rats. J Ethanopharmacol 1990; 9(1):25-34.

Dall’ Acqua S, Stocchero M, Scheavon M, Golob S, Uddin J, Voinovich D, et al. New findings on the in vivo antioxidant activities of Curcuma longa extract by an integrated(I)NMR and HPLC-MS metabolomic approach. Fitoterapia. 2016;109(3):125-31.