The study of skeletal muscle relaxant property of pheniramine maleate in acetylcholine induced contractions on isolated frog rectus muscle

Supriya Priyambada, Lakshmi Deepika P., Singamma M.


Background: A muscle relaxant is a drug which affects skeletal muscle function and decreases the muscle tone. It may be used to alleviate symptoms such as muscle spasm, pain and hyperreflexia. Skeletal muscle relaxants are heterogeneous group of medications that refer to 2 major therapeutic groups: neuromuscular blockers and spasmolytics. This study is carried out to evaluate the skeletal muscle property of Pheniramine maleate in Acetylcholine Induced Contractions on Isolated Frog Rectus Muscle.

Methods: There are various screening techniques available to assess the muscle relaxant property of a drug. For initial screening, frog rectus muscle is used. Here frogs are divided into 4 different groups. Each group contains 6 isolated frog rectus muscles. The experiment is carried out by adding 100μg, 200μg, 400μg and 800μg of pheniramine maleate with 80μg of acetylcholine to the organ bath and response is recorded by kymograph.

Results: Pheniramine maleate in various doses like 100μg, 200μg, 400μg and 800μg with 80μg of acetylcholine 100μg showed the maximum contractions of frog rectus muscle in kymograph. At all the doses of Pheniramine maleate, it showed a significant effect of skeletal muscle relaxant property.

Conclusions: In conclusion with work done by using pheniramine maleate in different doses along with 80μg of acetylcholine. Pheniramine maleate showed the maximum skeletal muscle relaxant property on frog rectus muscle at 800μg dose.


Isolated frog rectus muscle, Pheniramine maleate, Skeletal muscle relaxant property

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Bertam G. Katzung Skeletal muscle relaxant, in Basic and Clinical Pharmacology, 13th Edition; 2015:455.

Acharya SRK, Rao S. Action of scorpion venom on skeletal muscle and its antagonism by drugs, Arogya. J. Health Sciences; 1976:32:69-75.

Acharya SRK, Rao S. Action of chlorpromazine on the skeletal muscle of frog, current sciences. 1978:36:147-9.

Chandra V. Anticonvulsant Activity of Demethindane Maleate, Indian Journal of Pharmacology. 1972:32:194-7.

Gonzalez MA, Estes KS. Pharmacokinetic overview of oral second-generation H1 antihistamine, International Journal of Clinical Pharmacology. 1998:36:292-300.

Randal A. Skidgel, Allen P. Kaplan Histamines and their antagonists, in Goodman and Gilman The Pharmacological Basis of Therapeutics, 12th Edition; 2011:911.

Guyton and Hall, Neuromuscular transmission, Text book of Medical Physiology, 13th Edition; 2017:89-96.

Drugs used in treating motor disorders- pharmacology, in Rang HP, Dale MM Churchill Livingston London, 2nd Edition; 1991:684-705.

Salter JW, Zechnich AD, Haxby DG. Second-generation antihistamines: A Comparative Review Drugs, J Allergy ClinImmunol. 1999:41:31-47.

Acharya SRK, Rao S. Comparative Study of Antihistamines on Skeletal Muscles, Indian Journal of Pharmacology. 1977:6:132-8.

Rajendar B, Venkatesham A, Bhavana K, Divya C, Swarna M, Anvesh K. Evalution Of Skeletal Muscle Relaxant Activity of Methanolic Leaf Extract of Moringa Oleifera World Journal of Pharmacy and Pharmaceutical Sciences. 2017;6(5):1095-102.