Analgesic activity of Alpinia galanga extract in mice models and TNF-alpha receptor computational docking analysis on its leads with pharmacokinetics prediction

Authors

  • K. R. Subash Department of Pharmacology, SVIMS-Sri Padmavathi Medical College for Women, SVIMS University, Tirupati, Andhra Pradesh, India
  • G. Francis Britto Department of Pharmacology, IRT-PMC, Sanatorium (post), Perundurai, Erode, Tamilnadu, India
  • Katari Sudheer Kumar Bioinformatics Centre, Department of Bioinformatics, SVIMS University, Tirupati-517507, Andhra Pradesh, India
  • Amineni Umamaheshwari Bioinformatics Centre, Department of Bioinformatics, SVIMS University, Tirupati-517507, Andhra Pradesh, India
  • Vijaya Chandra Reddy Konda Department of Pharmacology, SVIMS-Sri Padmavathi Medical College for Women, SVIMS University, Tirupati, Andhra Pradesh, India
  • Bhanu Prakash G. Department of Pharmacology, SVIMS-Sri Padmavathi Medical College for Women, SVIMS University, Tirupati, Andhra Pradesh, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20180657

Keywords:

Alpinia galanga, Formalin assay, Insilico, Nociception, QSAR

Abstract

Background: Alpinia galanga is an ayurvedic herb recognized and used across many traditional medicine systems for its analgesic and anti-inflammatory activity. The present study scientifically validates the potential anti nociceptive action of ethanolic extract of Alpinia galanga by chemical, neurogenic and inflammatory nociception model in mice followed by identification of potential lead compound by computational analysis.

Methods: The assessment of anti nociceptive action is evaluated by Acetic acid induced abdominal constrictions and Formalin assay on ethonolic extract of Alpinia galanga, followed by 20 compounds with known chemical structure of Alpinia galanga is subjected to computational analysis to predict possible lead compound with desirable pharmacokinetic and drug like features.

Results: The percentage inhibition rate of Aspirin (100mg/kg) was 82.15% compared to Alpinia galanga (100mg/kg) 19.63%, (200mg/kg) 33.02% and (400mg/kg) 57.13% by acetic acid induced abdominal constrictions antinociceptive mice model. Alpinia galanga 400mg/kg (71.70%) had comparable percentage inhibition of nociception to standard group indomethacin (88.71%) in formalin induced nociceptive mice model. Among 20 compounds screened for pharmacokinetic and drug like features, Galanal B had the binding free energy -56.664 when compared to control compound 2AZ5-56.000.

Conclusions: The Alpinia galanga extract had significant anti nociceptive activity and followed by computational analysis of 20 compounds with known chemical structure predicted Galanal B as lead compound with best insilico pharmacokinetic and drug like features.

References

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Published

2018-02-22

How to Cite

Subash, K. R., Britto, G. F., Kumar, K. S., Umamaheshwari, A., Reddy Konda, V. C., & G., B. P. (2018). Analgesic activity of Alpinia galanga extract in mice models and TNF-alpha receptor computational docking analysis on its leads with pharmacokinetics prediction. International Journal of Basic & Clinical Pharmacology, 7(3), 446–450. https://doi.org/10.18203/2319-2003.ijbcp20180657

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Original Research Articles