DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20150878

Anti-inflammatory and analgesic properties of Ocimum sanctum: a comparative study using animal models

A. Umamageswari, B. L. Kudagi

Abstract


Background: Ocimum sanctum commonly known as Tulsi has been used for thousands of years in the Ayurveda for its diverse healing properties. This study was conducted to evaluate the anti-inflammatory and analgesic activity of O. sanctum aqueous leaf extract in thermal and chemical induced pain and inflammatory animal models.

Methods: Wistar albino rats (150-200 g) and swiss albino mice (25-30 g) were randomly divided into 4 groups of 6 animals each. The control group, test group, and standard drugs group received normal saline, O. sanctum extract (100 mg/kg), aspirin, and celecoxib respectively, by oral feeding. The anti-inflammatory effect was assessed by carrageenan induced rat paw edema and cotton pellet induced granuloma in rats. Analgesic effect was assessed by hot plate method and acetic acid induced writhing method in mice.

Results: In carrageenan induced rat paw edema, maximum inhibition by O. sanctum, aspirin, and celecoxib were 13.43%, 30%, and 32%, respectively, and time to reach maximum inhibition for O. sanctum was 2 hrs. In cotton pellet induced granuloma, percentage inhibition by O. sanctum, aspirin, and celecoxib were 23.85%, 45.84%, and 42.77%, respectively. In hot plate method, maximum inhibition by O. sanctum, aspirin and celecoxib were 143.92%, 288.18%, and 260.59%, respectively. In acetic acid induced writhing method, percentage protection by O. sanctum, aspirin, and celecoxib were 50.2%, 71.4%, and 66.5%, respectively.

Conclusion: The current study demonstrates statistically significant anti-inflammatory and analgesic activity of O. sanctum.


Keywords


Ocimum sanctum, Carrageenan, Granuloma, Writhing, Hot plate

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References


Warrier PK. In: Longman O, editor. Indian Medicinal Plants. New Delhi: CBS Publication; 1995: 168.

Sen P. Therapeutic potential of Tulsi: from experience to facts. Drug News Views. 1993;1:15-21.

Gupta SK, Prakash J, Srivastava S. Validation of traditional claim of Tulsi, Ocimum sanctum Linn. as a medicinal plant. Indian J Exp Biol. 2002;40(7):765-73.

Khanna N, Bhatia J. Antinociceptive action of Ocimum sanctum (Tulsi) in mice: possible mechanisms involved. J Ethnopharmacol. 2003;88(2-3):293-6.

Kelm MA, Nair MG, Strasburg GM, DeWitt DL. Antioxidant and cyclooxygenase inhibitory phenolic compounds from Ocimum sanctum Linn. Phytomedicine. 2000;7(1):7-13.

Narendhirakannan RT, Subramanian S, Kandaswamy M. Biochemical evaluation of antidiabetogenic properties of some commonly used Indian plants on streptozotocin-induced diabetes in experimental rats. Clin Exp Pharmacol Physiol. 2006;33(12):1150-7.

Goel RK, Sairam K, Dorababu M, Prabha T, Rao ChV. Effect of standardized extract of Ocimum sanctum Linn. on gastric mucosal offensive and defensive factors. Indian J Exp Biol. 2005;43(8):715-21.

Sharm M, Kishore K, Gupta SK, Joshi S, Arya DS. Cardiaprotective potential of Ocimum sanctum Linn in isoproterenol induced myocardial infraction in rats. Mol Cell Biochem. 2001;498:39-46.

Trevisan MT, Vasconcelos Silva MG, Pfundstein B, Spiegelhalder B, Owen RW. Characterization of the volatile pattern and antioxidant capacity of essential oils from different species of the genus Ocimum. J Agric Food Chem. 2006;54(12):4378-82.

Mukherjee R, Dash PK, Ram GC. Immunotherapeutic potential of Ocimum sanctum (L) in bovine subclinical mastitis. Res Vet Sci. 2005;79(1):37-43.

Ahmed M, Ahamed RN, Aladakatti RH, Ghosesawar MG. Reversible anti-fertility effect of benzene extract of Ocimum sanctum leaves on sperm parameters and fructose content in rats. J Basic Clin Physiol Pharmacol. 2002;13(1):51-9.

Ghangale GR, Tushar M, Jadhav ND. Evaluation of antiulcer activity of Ocimum sanctum in rats. Vet World. 2009;2(12):465-6.

Mahabub-Uz-Zaman M, Ahmed NU, Akter R, Ahmed K, Aziz SI, Ahmed S. Studies on anti-inflammatory, antinociceptive and antipyretic activities of ethanol extract of Azadirrachta indica leaves. Bangladesh J Sci Ind Res. 2009;44(2):199-206.

Tharkar PR, Tatiya AU, Surana SJ, Bhajipale NS. Anti-inflammatory study of Wrightia tinctoria R.Br stem bark in experimental animal models. Int J PharmTech Res. 2010;2(4):2434-7.

Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Studies on the anti-inflammatory and analgesic activity of Cedrus deodara (Roxb.) Loud. wood oil. J Ethnopharmacol. 1999;65(1):21-7.

Asad M, Prasad K, Thomas L, Kamath JV. Evaluation of analgesic and anti-inflammatory activity of sudard a poly herbal formulation. Int J Pharm Technol. 2007;6:71-5.

Karthikeyan K, Ravichandran P, Govindasamy S. Chemopreventive effect of Ocimum sanctum on DMBA-induced hamster buccal pouch carcinogenesis. Oral Oncol. 1999;35(1):112-9.

Asongalem EA, Foyet HS, Ekobo S, Dimo T, Kamtchouing P. Anti-inflammatory, lack of central analgesia and antipyretic properties of Acanthus montanus (Ness) T. Anderson. J Ethnopharmacol. 2004;95(1):63-8.

Perianayagam JB, Sharma SK, Pillai KK. Anti-inflammatory activity of Trichodesma indicum root extract in experimental animals. J Ethnopharmacol. 2006;104(3):410-4.

Thakur K. Anti-inflammatory activity of extracted eugenol from Ocimum sanctum leaves. Rass J Chem. 2009;2(2):472-4.

Singh S. Comparative evaluation of anti-inflammatory potential of fixed oil of different species of Ocimum and its possible mechanism of action. Indian J Exp Biol. 1998;36(10):1028-31.

D’arcy PF, Howard EM, Muggleton PW, Townsend SB. The anti-inflammatory action of griseofulvin in experimental animals. J Pharm Pharmacol. 1960;12:659-65.

Varde AB, Ainapure SS, Naik SR, Amladi SR. Anti-inflammatory activity of coconut oil extract of Acorus calamus, Ocimum sanctum and O. basilicum in rats. Indian Drugs. 1988;25(6):226-8.

Godhwani S, Godhwani JL, Vyas DS. Ocimum sanctum: an experimental study evaluating its anti-inflammatory, analgesic and antipyretic activity in animals. J Ethnopharmacol. 1987;21(2):153-63.

Kumar V, Sinha M, Banerjee A, Mohanty J. Antinociceptive activity of methanolic extract of Ocimum gratissimum (labiate) on experimental animals. Int J Pharm Pharm Sci. 2011;3(3):64-6.

Satyanarayana PS, Jain NK, Singh S, Kulkarni SK. Effect of selective inhibition of cyclooxygenase-2 on lipopolysa ccharide-induced hyperalgesia. Inflammopharmacology. 2004;12(1):57-68.