DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20175695

Evaluation of efficacy of Carica papaya leaf extracts to increase platelet count in hydroxyurea induced thrombocytopenia in Albino rats

M. Santhosh Kumar, Geetha M., Mansi J. Shah, Malvika Goyal, Srinivas L. D.

Abstract


Background: Dengue is an infectious disease associated with high mortality and morbidity. Being a viral disease, there is no specific drug available for treatment. There are some reports that Carica papaya leaf extract may improve the clinical condition of dengue patients. However, to support this, at present, there is no systematically searched and synthesized evidence available. Hence this study was undertaken to compare the efficacy of commercial preparation of Carica papaya leaves with freshly prepared Carica papaya leaf extracted.

Methods: 48 albino rats were randomly divided into eight groups of six each. Thrombocytopenia was induced by giving hydroxyurea (15mg/kg) orally. Group I and II served as saline and toxic control group respectively. Other six groups were given two different doses of either commercial extract or fresh extract orally for five days. 1ml of blood was withdrawn at baseline,3rd and 6th day of the study. Platelet, WBC, RBC count, clotting and bleeding time were determined.

Results: Mean platelet count increased significantly on day 6 in both low dose (2.06 to 4.93lakh/mm3) and human equivalent dose (2.73 to 7.66lakh/mm3) of commercial extract groups compared to the toxic control group (p<0.05). Similarly, the mean platelet count increased significantly for human equivalent dose in fresh leaf extract group (3.17 to 4.69lakh/mm3) but the increase in low dose fresh extract (3.28 to 3.76lakh/mm3) was not significant. There was no significant rise in mean platelets count, mean RBC count, WBC count, decrease in mean bleeding and clotting time between commercial extract and fresh leaf extract group for both low dose and human equivalent dose.

Conclusions: Efficacy of fresh leaf extract of Carica papaya was not inferior to commercial available preparation. Fresh Carica papaya leaf extract no doubt offers a potential therapeutic efficacy which is cost effective, more affordable and accessible treatment in patients with thrombocytopenia.


Keywords


Carica papaya, Platelets, Thrombocytopenia and Hydroxyurea

Full Text:

PDF

References


Shirtcliffe P, Cameron E, Nicholson K, Wiselka M. Don't forget dengue! Clinical features of dengue fever in returning travellers. J R Coll Physicians Lond. 1998;32(3):235-7.

Craig JIO, McClelland BDL, Ludlam CA. Blood disease. In: Boon NA, Colledge NR, Walkes BR, eds. Davidson’s principles and practice of medicine: 21st Ed. Churchill Livingstone: Elsevier; 2010:985-1051.

Sathasivam K, Ramanathan S, Mansor SM, Haris MRMH, Wernsdorfer WH. Thrombocyte counts in mice after the administration of papaya leaf suspension. Wien Klin Wochenschr. 2009; 121:19-22.

Patric T, Reddy VB, Grossman EJ, Hammes MS, Trevino HS, Ferrell J, et al. A prospective comparison of three argatroban treatment regimens during hemodialysis in end- stage renal disease. Kidney Int. 2004;66:2446-53.

Tefferi A, Michiels JJ, Barui T, Finazzi G, Fuchtman SM, Kutti J, et al. Diagnosis and Treatment of Polycythemia Vera and Possible Future Study Designs of the PVSG. Informa Healthcare. 2000;36:239-53.

Bussel JB, Kimberly RP, Inman RD, Schulman I, Cunningham-Rundles C, Cheung N, et al. Intravenous gammaglobulin treatment of chronic idiopathic thrombocytopenic purpura. J Am society Haematol. 1983.

Nishant N, Mohanty PK, Dengue SL. Papaya Leaf Is the Cure. Int J Life Sci Res. 2014;2:28-31.

Gammulle A, Ratnasooriya WD, Jayakody JRAC, Fernando C, Kanatiwela C, Preethi V. Thrombocytosis and Anti-inflammatory Properties, and Toxicological Evaluation of Carica papaya Mature Leaf Concentrate in a Murine Model. Online Int J Med Plant Res. 2012;1(2):21-30.

Gowda AC, Kumar V, Kasture PN, Nagabhushan KH. A pilot study to evaluate the effectiveness of Carica papaya leaf extract in increasing the platelet count in cases of dengue with thrombocytopenia. Ind Med Gazette. 2014:1-7.

Dhavan BN, Sirimal RC. Laboratory manual for pharmaceutical evaluation of natural products. International centre for Science and high technology. 2000;7-55.

Patil S, Shetty S, Bhide R, Narayanan S. Evaluation of Platelet Augmentation Activity of Carica papaya Leaf Aqueous Extract in Rats. Journal of Pharmacognosy and Phytochemistry. 2013;5:57-60.

Guruprasad NB, Dasaraju R. Efffect of Carica papaya leaf extract on hydroxyurea induces hematological and biological parameters in wistar albino rats. 2015;26:1-5.

Gammulle A, Ratnasooriya WD, Jayakody JRAC, Fernando C, Kanatiwela C, Preethi V. Udagama. Thrombocytosis and Anti-inflammatory Properties, and Toxicological Evaluation of Carica papaya Mature Leaf Concentrate in a Murine Model. 2012;1:21-30.