DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20174799

Effects of Ipomoea aquatica Forsk. in cyclophosphamide induced dyslipidaemia in albino rats

Rajesh Das, Khuraijam Sucheta Devi, Sayeri Dutta, Ananya Das, Prasenjit Das, Kh Krishna Pramodini Devi

Abstract


Background: Cyclophosphamide (CP) is commonly used as anticancer and immuno suppressant agent. It induces hyperlipidemia and myocardium damage. Ipomoea aquatic Forsk. is traditionally used for cardiovascular disease, paralysis and general debility. The present study was done to evaluate the protective effect of the plant against CP induced dyslipidaemia in albino rats.

Methods: Twenty albino rats were divided into 4 groups of 5 animals each. Group I (normal group) received normal saline intraperitoneally. Groups II to IV received CP (200mg/kg body weight, intraperitoneally) single dose on day 1 of experimental period. Groups I and II (toxic group) animals were given 2% gum acacia per orally daily for 10 days. Groups III and IV received aqueous extract of stem and leaves of Ipomoea aquatica 200mg/kg and 400mg/kg per orally respectively daily for 10 days. On 11th day, blood samples were collected for estimation of triglycerides, total cholesterol, LDL-cholesterol and HDL-cholesterol and heart tissues were sent for histopathology examination (HPE).

Results: CP administration significantly (P<0.05) increased the levels of triglyceride, total cholesterol, LDL-cholesterol and decreased the level of HDL-cholesterol in toxic group compared to normal group. Treatment with aqueous extract of Ipomoea aquatica significantly (P<0.05) reversed the status of lipid profile compared to toxic group. HPE of myocardium showed reversal of the toxic effects of CP in the extract treated groups.

Conclusions: Ipomoea aquatica showed protective effects against CP induced dyslipidaemia in albino rats.


Keywords


Albino rats, Cyclophosphamide, Dyslipidaemia, Ipomoea aquatic Forsk.

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References


Chu E, Sartorelli AC. Cancer chemotherapy. In: Katzung BG, Trevor AJ, editors. Basic and clinical pharmacology. 13th Ed. New Delhi: McGraw-Hill Education; 2015:918-945.

Taghiabadi E, Imenshahidi M, Abnous K, Mosafa F, Sankian M, Bahram Memar B, et al. Protective effect of silymarin against acrolein-induced cardiotoxicity in mice. Evid Based Complement Alternat Med. 2012;2012:1-14.

Conklin DJ, Barski OA, Lesgards JF, Juvan P, Rezen T, Rozman D, et al. Acrolein consumption induces systemic dyslipidemia and lipoprotein modification. Toxicol Appl Pharmacol. 2010;243(1):1-26.

Malakarc C, Choudhury PPN. Pharmacological potentiality and medical uses of Ipomoea aquatica Forsk: a review. Asian J Pharm Clin Res. 2015;8(2):60-3.

Manvar MN. Pharmacognostical investigations on Ipomoea aquatica Forsk. Int J Pharm Sci Res. 2011;2(11):2812-5.

Sinha SC. Medicinal plants of Manipur. Imphal: Mass & Sinha; 1996.

Verma SCL, Agarwal SL. Studies on Leptadenia reticulata: Part II. Preliminary chemical investigations. Indian J Med Res. 1962;50:439-50.

OECDi Library. Fact sheet: OECD guidelines for the testing of chemicals, Section 4. Test no. 423: Acute Oral toxicity- Acute toxic class method. Available at: http://www.oecd-ilibrary.org/environment/test-no-423-acute-oral-toxicity-acute-toxic-class-method_9789264071001-en Accessed 16 August 2017.

Ministry of Environment, Forest and Climate Change, Government of India. Fact sheet: Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA). Standard Operating Procedures (SOP) for Institutional Animal Ethics Committee (IAEC) of the CPCSEA. Available at: http://www.moef.nic.in/division/committee-purpose-control-and-supervision-experiments-animals-cpcsea Accessed 16 August 2017.

Sullivan DR, Kruijswijk Z, West CE, Kohlmeier M, Katan MB. Determination of serum triglycerides by an accurate enzymatic method not affected by free glycerol. Clin Chem. 1985;31(7):1227-8.

Allain CC, Poon LS, Chan CS, Richmond W, Fu CP. Enzymatic determination of total serum cholesterol. Clin Chem. 1974;20:470-5.

Grub F, Izzo C, Mazzotti G, Murador E. Improved method for determination of high-density-lipoprotein cholesterol II. Enzymic determination of cholesterol in high-density lipoprotein fractions with a sensitive reagent. Clin Chem. 1981;27(3):375-9.

Ito MK. Dyslipidaemias. In: Burns MAC, Wells BG, Schwinghammer TL, Malone PM, Kolesar JM, Di Piro JT, editors. Pharmacotherapy: principles and practice. 3rd Ed. New York: Mc Graw Hill medical; 2013:245-267.

Moirangthem RS, Jitendra SSV, Devi HC, Devi KS, Gunindro N, Devi NM. Effect of aqueous extract of Phyllanthus fraternus leaf against cyclophosphamide induced dyslipidemia and aortitis in wistar albino rats. Int J Cont Med Res. 2016;3(9):2703-6.

Grizzle WE, Fredenburgh JL, Myers RB. Fixation of tissues. In: Bancroft JD, Gamble M, editors. Theory and practice of histological techniques. 6th Ed. Philadelphia: Churchill Livingstone; 2008:53-74.

Spencer LT, Bancroft JD. Microtomy: paraffin and frozen. In: Bancroft JD, Gamble M, editors. Theory and practice of histological techniques. 6th Ed. Philadelphia: Churchill Livingstone; 2008:93-104.

Jones ML, Bancroft JD, Gamble M. Connective tissues and stains. In: Bancroft JD, Gamble M, editors. Theory and practice of histological techniques. 6th Ed. Philadelphia: Churchill Livingstone; 2008:135-160.

Bersot TP. Drug therapy for hypercholesterolemia and dyslipidemia. In: Brunton LL, Chabner BA, Knollmann BC, editors. Goodman & Gilman's The Pharmacological Basis of Therapeutics. 12th Ed. New York: McGraw-Hill Medical; 2011:877-908.

Gado AM, Adam ANI, Aldahmash BA. Cardiotoxicity induced by cyclophosphamide in rats: protective effect of curcumin. J Res Environ Sci Toxicol. 2013;2(4):87-95.

Swamy AHMV, Patel UMP, Koti BC, Gadad PC, Patel NL, Thippeswamy AHM. Cardioprotective effect of Saraca indica against cyclophosphamide induced cardiotoxicity in rats: a biochemical, electrocardiographic and histopathological study. Indian J Pharmacol. 2013;45(1):44-8.

Sivaraman D, Muralidaran P. Hypolipidemic activity of Ipomoea aquatica Forsk. leaf extracts on lipid profile in hyperlipidemic rats. Int J Pharm Biol Sci Arch. 2010;1(2):175-9.

Manvar MN, Desai TR. Phytochemical and pharmacological profile of Ipomoea aquatica. Indian J Med Sci. 2013;67(3):49-60.

Prasad KN, Divakar S, Shivamurthy GR, Aradhya SM. Isolation of a free radical-scavenging antioxidant from water spinach (Ipomoea aquatica Forsk). J Sci Food Agric. 2005;85:1461-8.