Isoniazid induced hepatotoxicity and its amelioration with ethanolic extract of stem bark of Berberis lycium Royale in mice

Authors

  • Saima Rafiq Department of Pharmacology, Wah Medical College, Wah Cantt, Pakistan
  • Khalida Ajmal Department of Pharmacology, Wah Medical College, Wah Cantt, Pakistan
  • Ayesha Afzal Department of Pharmacology, Wah Medical College, Wah Cantt, Pakistan

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20173270

Keywords:

Berberis lycium Royale, Hepatoprotective, Isoniazid (INH), LFT’s, Steatosis

Abstract

Background: To study the hepatoprotective effect of Ethanolic extract of Stem Bark of Berberis lycium Royale in isoniazid (INH) induced hepatotoxicity in mice model.

Methods: The study design was lab based randomized controlled in-vivo study in mice conducted from 9th April 2014 till 9th May 2014 at animal house of National Institute of Health, Islamabad. Group A was on normal diet and water and hepatotoxicity was produced by giving isoniazid (50mg/kg BW) in mice of Group B. Group C and D were given isoniazid (INH) plus low dose and high dose of Ethanolic extract of stem bark of Berberis Lycium Royle respectively.

Results: INH induced hepatotoxicity was depicted by elevated serum LFT’s, hepatocytic ballooning, severe steatosis and inflammation. Mice getting concurrent treatment of INH, low and high dose of Ethanolic extract of Berberis Lycium Royle showed decreased serum levels of biomarkers and their liver sections manifested improved histological picture but more significant reduction in toxic effects were observed in animals receiving high dose.

Conclusions: High dose of Ethanolic extract of stem bark of Berberis lycium Royale showed more marked hepatoprotective activity as compare to low doses. The hepatotoxicity of INH can be reduced by concurrent use of INH with ethanolic extracts of Berberis Lycium Royle.

References

Miesel L, Rozwarski DA, Sacchettini JC, Jacobs WR, editors. Mechanisms for isoniazid action and resistance. Genetics and Tuberculosis: Novartis Foundation Symposium, Wiley Online Library; 1998:217.

Kumar A. A review on hepatoprotective herbal drugs. IJRPC. 2012;2:92-102.

Russmann S, Kullak-Ublick GA, Grattagliano I. Current concepts of mechanisms in drug-induced hepatotoxicity. Current medicinal chemistry. 2009;16:3041.

Metushi I, Cai P, Zhu X, Nakagawa T, Uetrecht J. A Fresh Look at the Mechanism of Isoniazid‐Induced Hepatotoxicity. Clinical Pharmacology and Therapeutics. 2011;89:911-4.

Hassan AS, Ahmed JH, Al-Haroon SS. A study of the effect of Nigella sativa (Black seeds) in isoniazid (INH)- induced hepatotoxicity in rabbits. Indian journal of pharmacology. 2012;44:678.

Maryam S, Aziz K, Bhatti ASA, Shahzad AW. Effects of Jetepar (glucometamine, glucodiamine and nicotinamide ascorbate) on Isoniazid Induced Hepatotoxicity in Rabbits. Annals of King Edward Medical University. 2010;16:37-42.

Tayal V, Kalra BS, Agarwal S, Khurana N, Gupta U. Hepatoprotective effect of tocopherol against isoniazid and rifampicin induced hepatotoxicity in albino rabbits. Indian journal of experimental biology. 2007;45:1031.

Shabbir A, Shahzad M, Arfat Y, Ali L, Aziz RS, Murtaza G, et al. Berberis lycium Royle: A review of its traditional uses, phytochemistry and pharmacology. African Journal of Pharmacy and Pharmacology. 2012;6:2346-53.

Murad W, Ahmad A, Gilani SA, Khan MA. Indigenous knowledge and folk use of medicinal plants by the tribal communities of Hazar Nao Forest, Malakand District, North Pakistan. Journal of Medicinal Plants Research. 2011;5:1072-86.

Janbaz K, Gilani A. Studies on preventive and curative effects of berberine on chemical-induced hepatotoxicity in rodents. Fitoterapia. 2000;71(1):25-33.

Girish C, Koner B, Jayanthi S, Rao K, Rajesh B, Pradhan S. Hepatoprotective activity of six polyherbal formulations in paracetamol induced liver toxicity in mice. Indian Journal of Medical Research. 2009;129:569-78.

Yin J, Zhang H, Ye J. Traditional Chinese medicine in treatment of metabolic syndrome. Endocrine, metabolic and immune disorders drug targets. 2008;8:99.

Ahmed E, Arshad M, Ahmad M, Saeed M, Ishaque M. Ethnopharmacological survey of some medicinally important plants of Galliyat Areas of NWFP, Pakistan. Asian Journal of Plant Sciences. 2004;3:410-5.

Pal R, Valphei K, Singh K, Rana S. Garlic confers hepatoprotection in isoniazid rifampicin induced hepatic injury. Ind J Gastro. 2003;1:A100.

Mashwani ZUR, Khan MA, Irum S, Ahmad M. Antioxidant potential of root bark of Berberis lycium Royle. From galliyat, western himalaya, pakistan. Pakistan journal of botany. 2013;45:231-4.

Akpanabiatu MI UIB, Udosen EO, Udon AE. Rat serum electrolytes, lipid profile and cardiovascular activity on nuclea latifolia leaf extract administration. Indian J Clin Biochem; 2005.

Y De. Effects of drugs on clinical laboratorytests: AACC Press Washington; 1995.

Tietz NW. Clinical guide to laboratory tests: WB Saunders Co; 1995.

Issabeagloo E, Taghizadieh M. Hepatomodulatory Action of Camellia sinensis Aqueous Extract against Isoniazid-Rifampicin Combination Induced Oxidative Stress in Rat. Advances in Bioresearch. 2012;3(3).

Sodhi C, Rana S, Attri S, Mehta S, Yaiphei K, Mehta S. Oxidative-Hepatic Injury of Isoniazid-Rifampicin in Young Rats Subjected to Protein and Energy Malnutrition. Drug and chemical toxicology. 1998;21(3):305-17.

Agrawal S, Kulkarni G, Sharma V. Antimicrobial and anti-inflammatory activities of bark of four plant species from Indian Origin. 2012.

Hoareau L, DaSilva EJ. Medicinal plants: a re-emerging health aid. Electronic Journal of Biotechnology. 1999;2(2):3-4.

Thatte U, Bhalerao S. Pharmacovigilance of ayurvedic medicines in India. Indian journal of pharmacology. 2008;40(1):S10.

Singh N. A comparison of both water and ethanol extracts prepared from Echinacea purpurea and Echinacea angustifolia on the response to Influenza A/PR/8/34 infection in mice. 2010.

Chand N, Durrani FR, Ahmad S, Khan A. Immunomodulatory and hepatoprotective role of feed‐added Berberis lycium in broiler chicks. Journal of the Science of Food and Agriculture. 2011;91(10):1737-45.

Kim TS, Kang BY, Cho D, Kim SH. Induction of interleukin‐12 production in mouse macrophages by berberine, a benzodioxoloquinolizine alkaloid, deviates CD4+ T cells from a Th2 to a Th1 response. Immunology. 2003;109(3):407-14.

Fernández-Villar A, Sopeña B, Vázquez R, Ulloa F, Fluiters E, Mosteiro M, et al. Isoniazid hepatotoxicity among drug users: the role of hepatitis C. Clinical infectious diseases. 2003;36(3):293-8.

Ahmad MA, Chaudhary MZ, Nadeem M, Sharif T, Ahmad B. Hepatoprotective effect of Berberis lycium (Royle) in hepatotoxic rabbits. Gomal Uni J. 2008:24.

Ahmed A. Biochemical Studies on Berberis Lyceum Royal and Analysis of its Extracts for Bioactivity: University of Arid Agriculture, Rawalpindi; 2009.

Imber CJ, St Peter SD, Handa A, Friend PJ. Hepatic steatosis and its relationship to transplantation. Liver transplantation. 2002;8(5):415-23.

Hübscher S. Histological assessment of non‐alcoholic fatty liver disease. Histopathology. 2006;49(5):450-65.

Navarro MC MM, Martin A, Jimenez. J, Utrilla MP. Free radicals and antihepatotoxic activity of Rose measuring tomentosus. Planta Medica; 1992.

Downloads

Published

2017-07-22

How to Cite

Rafiq, S., Ajmal, K., & Afzal, A. (2017). Isoniazid induced hepatotoxicity and its amelioration with ethanolic extract of stem bark of Berberis lycium Royale in mice. International Journal of Basic & Clinical Pharmacology, 6(8), 1865–1870. https://doi.org/10.18203/2319-2003.ijbcp20173270

Issue

Section

Original Research Articles