DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20175201

Cupping: a reasonable choice for attenuating morphine withdrawal symptoms in Wistar rats

Parinaz Khosravi, Mohammad M. Roostayi, Farideh Dehghan Manshadi, Khosro Khademi-Kalantari, Kambiz Hassanzadeh, Alireza Akbarzadeh Baghban

Abstract


Background: Nowadays, the sedative drugs are the main treatments to attenuate the opioid withdrawal symptoms. However, physical therapies are found a safe alternative treatment without any serious adverse effects. This study aimed to evaluate the effects of cupping treatment on the morphine withdrawal symptoms in rats.

Methods: Eighty rats were divided into two main groups; treated with morphine or saline, and each group was also divided to cupping and sham cupping subgroups (with single or daily treatment). Injection of morphine and saline were done in the morphine and saline groups twice a day for nine days. On the ninth day, the naloxone was administered and the withdrawal symptoms including jumping, rearing, body grooming, abdominal writing, and wet-dog shaking were recorded for 60 minutes. Cupping or sham cupping was applied on the GV14 once before or daily after withdrawal induction in each group.

Results: The results revealed that a single cupping before the withdrawal induction significantly attenuated the withdrawal symptoms in the morphine group (p<0.01).But, the daily cupping failed to decrease the withdrawal symptoms in this group. The results also indicated that the daily cupping in the saline group increased the naloxone-induced withdrawal symptoms significantly (p<0.01).

Conclusions: Single session cupping before the withdrawal induction could decreased the withdrawal symptoms. This finding might be related to the modulation of GABAergic system.


Keywords


Cupping, Morphine, Withdrawal symptoms

Full Text:

PDF

References


Rang H, Dale M, Ritter J. Rang & Dale's Pharmacology. 7th Ed. London: University College London; 2011.

Habibi Asl B, Hassanzadeh K, Khezri E, Mohammadi S. Evaluation the effects of dextromethorphanand midazolam on morphine induced tolerance and dependence in mice. Pak J Biol Sci.2008;11(13):1690-5.

Chen Y, Sommer C. The role of mitogen-activated protein kinase (MAPK) in morphine tolerance and dependence. Mol Neurobiol. 2009;(40):101-7.

Habibi Asl B, Hassanzadeh K. Effects of ketamine and midazolam on morphine induced dependence and tolerance in mice. DARU. 2004;(12):101-5.

Katzung B. Basic and clinical pharmacology, chapter 31. 12th Ed. New York: Mc Graw-hill; 2012.

Chen CJ, Yu HS. Acupuncture, electrostimulation, and reflex therapy in dermatology. Dermatol Ther. 2003;16(2):87-92.

Hssanien MMR, Mansoura Fawaz S, Ahmed AF, Al Emadi S. Effect of cupping therapy in treating chronic headache and chronic back pain at Al heijamah clinic HMC. World Family Med J. 2010;8(3):30-6.

Lee JH, Kim HY, Jang EY, Choi SH, Han CH, Lee BH, et al. Effect of acupuncture on naloxone-precipitated withdrawal syndrome in morphine-experienced rats: the mediation of GABA receptors. Neurosci Lett. 2011;504(3):301-5.

Yoon SS, Kim H, Choi KH, Lee BH, Lee YK, Lim SC, et al. Acupuncture suppresses morphine self-administration through the GABA receptors. Brain Res Bull. 2010;81(6):625-30.

Ullah K, Younis A, Wali M. An investigation into the effect of Cupping Therapy as a treatment for Anterior Knee Pain and its potential role in Health Promotion. The Internet Journal of Alternative Medicine. 2006;4(1).

Roostayi MM, Bagheri H, Talebian MS, Firooznia K, Razi M, Hosseini M, et al. The effects of vacuumic bracing system on the patellofemoral articulation in patients with patellofemoral pain syndrome Complement. Ther Clin Pract. 2009;15(1):29-34.

Kim JI, Lee MS, Lee DH, Boddy K, Ernst E. Cupping for Treating Pain: A Systematic Review. Evidence Based Complementary and Alternative Medicine; 2009.

Roostayi MM, Norouzali T, Dehghan MF, Akbarzadeh BA. The Effects of Cupping Therapy on Skin’s Biomechanical Properties in Wistar Rats. Chinese Medicine. 2016;(7):25-30.

Kim ST, Moon W, Chae Y, Kim YJ, Lee H, Park HJ. The effect of electroaucpuncture for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced proteomic changes in the mouse striatum. J Physiol Sci. 2010;(60):27-34.

Xu YD, Cui JM, Wang Y, Yin LM, Gao CK, Liu XY, et al. Proteomic analysis reveals the deregulation of inflammation- related proteins in acupuncture-treated rats with asthma onset. Evid Based Complement Alternat Med. 2012.

Habibi Asl B, Sadegh amiri O, Charkhpour M, Hasanzadeh K. A novel pharmacological role for minocycline: attenuating the withdrawal syndrome of morphine in rat. Pharmaceutical Sciences. 2009;(15):67-74.

Riahi E, Mirzaii DI, Karimian SM, Sadeghipour Roodsari HR, Dehpour AR. Attenuation of morphine withdrawal signs by a GABAB receptor agonist in the locus coeruleus of rats. Behav Brain Res. 2009;196(1):11-4.

Crawley JN, Gerfen GR, Rogawski MA, Sibley DR, Skolnick P, Wray S. Short protocols in neuroscience. John Wiley & Sons Inc. 2007.

Charkhpour M, Nayebi AM. Evaluation of the role of 5-HT2 receptors in dorsal and medianraphe nuclei on the morphine withdrawal syndrome in rat. Pharmaceutical Sciences. 2006;(12):33-40.

Michalsen A, Bock S, ludtke R. Effectiveness of cupping therapy in brachialgia paraestetica nocturna: results of a randomized controlled trial. Forsch Komplementarmed. 2007;14:19.

Schulte E. Complementary therapies: Acupuncture: Where East meets west. Res Nurs. 1996;59:55-7.

Cadwell V. A primer on acupuncture. J Emerg Nurs. 1998;(24):514-7.