Comparative study of topical application of nanosilver and human placental extract on wound healing in rabbits

Umamaheshwara Raju S., T. S. Usha Sree, Karuna Sree Podila, Vijay Krishna


Background: The objective of the study was to study the comparative effect of topical application of nanosilver and human placental extract on wound healing in rabbits.

Methods: 12 rabbits were randomly divided into two groups (n=6) and wound healing effect was observed in Excision model. Standard group was treated with topical human placental extract gel and test group was treated with topical Nanosilver cream. The mean percentage of the wound healed ‘within’ the group and ‘in between’ the groups were observed on day 3, 6, 9, 12, 15, 18, 21 and biopsy was done on day 21 for histopathological examination.

Results: The mean percentage of wound contracted with Human placental extract vs nanosilver was 29.5 vs 35.7 on day3, 46.2 vs 53.5 on day 6, 61.5 vs 70.2 on day9, 74.2 vs 88.0 on day12, 89.5 vs 97.0 on day15, 96.7 vs 99.9 on day 18 and 99.9 vs 100 day 21 respectively. The mean percentage of the wound contracted when day1 compared to day21 was statistically significant (P value 0.0001) within the groups but in between the groups it was statistically non significant (P >0.05). There was an increase in collagen content and granulation tissue in the histopathological examination in both the groups on day 21.

Conclusions: Wound healing effect of the Nanosilver was comparable to that of Human placental extract in excision wound model of rabbit.


Human placental extract, Nanosilver, Wound healing

Full Text:



Robbins and Cotran. Tissue Renewal, Regeneration, and Repair; Robbins and Cotran Pathologic Basis of Disease. 8th Edition; Chapter 3; 2011:79-108.

Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev. 2001;(14):244-69.

Shukla K, Ansari MA. An Indian community based epidemiological study of wounds. J Wound Care. 2004;(13):323-5.

Sasank CS. Venous ulcer of lower limb, where do we stand?. Indian J Plast Surg. 2012;48(2):260-74.

Alam G. Wound healing potential of some medicinal plants. Int J Pharm Sci Rev Res. 2011 July-Aug;9(1).

Davidson JM. Experimental Animal Wound Models. Wounds; © 2001 Health Management Publications, Inc. 2001;13(1):1-6.

Chandanwale A, Langade D, Mohod V, Sinha S, Ramteke A, Bakhshi GD, et al. Comparative evaluation of human placental extract for its healing potential in surgical wounds after orthopaedic surgery: an open, randomised, comparative study. J Indian Med Assoc. 2008 Jun;106(6):405-8.

Klasen HJ. Historical review of the use of silver in the treatment of burns: early uses. Burns. 2000;26:117-30.

Patra CR, Bhattacharya R, Wang E. Targeted delivery of gemcitabine to pancreatic adenocarcinoma using cetuximab as a targeting agent. Cancer Res. 2008;68:1970-8.

Silver S. Bacterial silver resistance: molecular biology and uses and misuses of silver compounds. FEMS Microbiol Rev. 2003;27:341-53.

Shrivastava S. Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology. 2007;18:225103-12.

Gunasekaran T, Nigusse T, Dhanaraju MD. Silver nanoparticles as real topical bullets for wound healing. J Am Coll Clin Wound Spec. 2012 Jun 4;3(4):82-96.

Asharani PV, Hande MP, Valiyaveettil S. Anti-proliferative activity of silver nanoparticles. BMC Cell Biol. 2009;10-65.

Biswas TK, Auddy B, Bhattacharya NP, Bhattacharya S, Mukherjee B. Wound healing activity of human placental extracts in rats. Acta Pharmacol Sin. 2001 Dec;22(12):1113-6.