Comparative evaluation of anti-diabetic activity of fresh juice and ethanolic extract of Sunderban mangrove Rhizophora mucronata Lam. leaves in animal model

Anjan Adhikari; Moumita Ray; Tapas K. Sur; Sharmistha Biswas; Anup K. Das

doi:10.18203/2319-2003.ijbcp20173743

Authors

Anjan Adhikari Department of Pharmacology, R. G. Kar Medical College, 1-Kshudiram Bose Sarani, Kolkata 700004, West Bengal, India
Moumita Ray Department of Pharmacology, R. G. Kar Medical College, 1-Kshudiram Bose Sarani, Kolkata 700004, West Bengal, India
Tapas K. Sur Department of Pharmacology, Institute of Post Graduate Medical Education and Research, 244, A.J.C. Bose Road, Kolkata 700020, West Bengal, India
Sharmistha Biswas Department of Anatomy, Malda Medical College, Englishbazar, Malda 732101, West Bengal, India
Anup K. Das Department of Pharmacology, R. G. Kar Medical College, 1-Kshudiram Bose Sarani, Kolkata 700004, West Bengal, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20173743

Keywords:

Anti-oxidant, Diabetes, Ethanol extract, Mangrove, Rhizophora mucronata

Abstract

Background: Mangrove flora possess compounds with potential medicinal values with unique bioactive components. Traditionally Rhizophora mucronata, a mangrove has been used extensively for the treatment of diabetes. Studies revealed that, the leaves of Rhizophora (Bhora) had promising anti-diabetic action in rat model.

Methods: A comparative analysis of the anti-diabetic action of fresh juice and ethanolic extract of Rhizophora mucronata leaves was carried out in Streptozotocin induced diabetic model and the different biochemical parameters were evaluated.

Results: Present research explored a comparative analysis of the anti-diabetic action of fresh juice and ethanolic extract of leaves of Rhizophora mucronata Lam. in Streptozotocin induced diabetic model. The ethanolic extract showed more potent effect in lowering the elevated blood sugar in the diabetic rats, 200mg/kg was the most effective dose for both the extracts. The ethanol extract was more beneficial having potent lipid lowering action along with anti-hyperglycemic property.

Conclusions: This supports the scientific validation for using Rhizophora mucronata leaves in the treatment of diabetes as traditional folk medicine. Identification of the bioactive molecule is under process.

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References

American Diabetes Association. Diagnosis and Classification of Diabetes Mellitus. Diabetes Care 2009;32(1):S62-7.

Rajaei Z, Hadjzadeh M, Moradi R, Ghorbani A, Saghebi A. Antihyperglycemic and antihyperlipidemic effects of hydroalcoholic extract of Securigera securidaca seeds in streptozotocin-induced diabetic rats. Adv Biomed Res. 2015;4:33.

Das SK, Samantaray D, Thatoi H. Ethnomedicinal, Antimicrobial and Antidiarrhoeal Studies on the Mangrove Plants of the Genus Xylocarpus: A Mini Review. J Bioanal Biomed. 2014;S12(004):1-7.

Bandaranayake WM. Bioactivities, bioactive compounds and chemical constituents of mangrove plants. Wetlands Ecol Mangt. 2002;10:421-52.

Duke JA, Wain KK. Medicinal plants of the World. Computer index with more than 85,000 entries. 1981;3:1654.

Ramanathan T, Hariharan B, Ganesan K. Antidiabetic activity of a coastal mangrove leaves of Rhizophora mucronata. Int J Plant Res: Plant Arch. 2008;8:931-3.

Pandey AK, Gupta PP, Lal VK. Hypoglycemic effect of Rhizophora mucronata in streptozotocin induced diabetic rats. J. Complement Integr Med. 2014;11:179-83.

Kumar S, Sandhir R, Ojha S. Evaluation of antioxidant activity and total phenol in different varieties of Lantana camara leaves. BMC Research Notes. 2014;7:560.

Adhikari A, Ray M, Sur TK, Kanjilal S, Roy RK, Das AK. Analgesic actions of Sunderban mangrove, Rhizophora mucronata L. leaves. Journal of Medicinal Plants Studies. 2016;4(3):140-3.

Ray M, Adhikari A, Sur TK, Mondal C, Pathak A, Das AK. Pharmacognostic and anti-hyperglycemic evaluation of the leaves of Sunderban mangrove, Rhizophora mucronata. Pharmanest. 2014;5:2289-94.

Gaffar MU, Morshed MA, Uddin A, Roy S, Hannan JMA. Study the efficacy of Rhizophora mucornata Poir. leaves for diabetes therapy in long evans rats. International Journal of Biomolecules and Biomedicine. 2011;1(1):20-6.

Sur TK, Hazra AK, Bhattacharyya D, Hazra A. Antiradical and antidiabetic properties of standardized extract of Sunderban mangrove Rhizophora mucronata. Phcog Mag. 2015;11:389-94.

Pandey AK, Gupta PP, Lal VK. Hypoglycemic effect of Rhizophora mucronata in streptozotocin induced diabetic rats. J Complement Integr Med. 2014;11(3):179-83.

Sur TK, Seal T, Pandit S, Bhattacharyya D. Hypoglycemic properties of a mangrove plant, Rhizophora apiculata Blume. Natural Product Sciences. 2004;10:11-5.

Akbarzadeh A, Norouzian D, Mehrabi MR, Jamshidi SH, Farhangi A, Verdi AA, et al. Induction of diabetes by streptozotocin in rats. Indian Journal of Clinical Biochemistry. 2007 Sep 1;22(2):60-4.

Gandhi GR, Sasikumar P. Antidiabetic effect of Merremia emarginata Burm. F. in streptozotocin induced diabetic rats. Asian Pac J Trop Biomed. 2012;2(4):281-6.

Kumar S, Kumar V, Prakash O. Antidiabetic and anti-lipemic effects of Cassia siamea leaves extract in streptozotocin induced diabetic rats. Asian Pac J Trop Med. 2010;3:871-3.

Wu J, Yan L. Streptozotocin-induced type 1 diabetes in rodents as a model for studying mitochondrial mechanisms of diabetic β cell glucotoxicity. Diabetes Metab Syndr Obes. 2015;8:181-8.

Ray M, Adhikari A, Sur TK, Biswas S, Roy RK, Das AK. Promising Blood Sugar Lowering Action of Sunderban Mangrove. Indian J Pharmacol. 2014;46(1):S62-113.

Wang Y, Xiang L, Wang C, Tang C, He X. Antidiabetic and antioxidant effects and phytochemicals of mulberry fruit (Morus alba L.) polyphenol enhanced extract. Plos One. 2013;8:e71144.

Ray M, Adhikari A, Sur TK, Besra SE, Biswas S, Das AK. Evaluation of Anti-Inflammatory Potential of Ethanolic Extract of the Leaves of Rhizophora Mucronata, a Sunderban Mangrove. Int J Res Dev Pharm L Sci. 2017;6(2):2510-1.

Vessal M, Hemmati M, Vasei M. Antidiabetic effects of quercetin in streptozocin-induced diabetic rats. Comparative Biochem Physiol C. 2003;135:357-64.

Abdelmoaty MA, Ibrahim MA, Ahmed NS, Abdelaziz MA. Confirmatory studies on the antioxidant and antidiabetic effect of quercetin in rats. Indian Journal of Clinical Biochemistry. 2010;25(2):188-92.