Protective effect of Berberis asiatica root on biochemical and histopathological changes in streptozotocin-induced diabetic Wistar rats

Authors

  • Mohd Muddassir Husain Khan Department of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
  • Chetan Rastogi Department of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India Department of Pharmacology, Hygia Institute of Pharmaceutical Education and Research, Lucknow, Uttar Pradesh, India
  • Sachin Gupta Department of Pharmacology, Advance Institute of Biotech and Paramedical Sciences, Kanpur, Uttar Pradesh, India
  • Shravan Kumar Paswan Department of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India Amity Institute of Pharmacy, Amity University, Lucknow, Uttar Pradesh, India
  • Pritt Verma Department of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India Amity Institute of Pharmacy, Amity University, Lucknow, Uttar Pradesh, India
  • Talha Jawaid Department of Pharmacology, Hygia Institute of Pharmaceutical Education and Research, Lucknow, Uttar Pradesh, India
  • Ch. V. Rao Department of Pharmacognosy and Ethnopharmacology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India

DOI:

https://doi.org/10.18203/2319-2003.ijbcp20173110

Keywords:

Anti-diabetic, Anti-oxidant, Berberis asiatica, Berberine

Abstract

Background: This study was designed to evaluate the effect of Berberis asiatica root extract (BAE) against streptozotocin induced elevated blood glucose level and other liver and kidney functions changes in adult male Wistar rats.

Methods: Thirty male Wistar rats were divided into five groups of six rats in each (Groups I-V). Group I and Group II served as normal control and disease control, respectively. Group III received standard anti-diabetic drug glibenclamide (5mg/kg), while Group IV and Group V received the low dose (250mg/kg) and high dose (500mg/kg) of BAE. Serum blood glucose, SGOT, SGPT, ALP, total bilirubin, BUN, serum creatinine, TC, TG, HDL-C, LDL-C, and VLDL-C were estimated using standard methods. After collection of samples for biochemical evaluation, the pancreas from each animal was isolated and examined for histological changes.

Results: BAE and glibenclamide treated disease rats showed significant (p <0.05) decrease in blood glucose concentration. Treatment with BAE at 250mg/kg and 500mg/kg in Group IV and V and standard drug glibenclamide in Group III showed significant (p <0.05) reduction in the level of liver function substances such as SGOT, SGPT, ALP and total bilirubin as compared to disease group, as well as showed significant (p <0.05) decrease in renal panel. Liver function parameters were significantly (p <0.05) improved in groups treated with BAE. Histopathological analysis revealed the protective effect of BAE against streptozotocin induced damage to islets of Langerhans.

Conclusions: This study showed the destruction of islets of Langerhans and elevation in blood glucose level as well as alteration in other biochemical parameters were ameliorated by the effect of Berberis asiatica extract.

References

McGinley C, Shafat A, Donnelly AE. Does antioxidant vitamin supplementation protect against muscle damage? Sports Med. 2009;39:1011-32.

King H, Aubert RE, Herman WH. Global burden of diabetes, 1995–2025: prevalence, numerical estimates, and projections. Diab Care. 1998;2:1414-31.

Yajnik CS. The insulin resistance epidemic in India: fetal origins, later lifestyle, or both? Nutri Rev-Washing. 2001;59:1-9.

Nyholm B, Pørksen N, Juhl C, Gravholt C, Butler P, Weeke J, et al. Assessment of insulin secretion in relatives of patients with type 2 (non-insulin-dependent) diabetes mellitus: evidence of early β-cell dysfunction. Metabol. 2000;49:896-905.

McGarry JD. What if Minkowski had been ageusic? An alternative angle on diabetes. Science. 1992;258:766-70.

Reaven GM. Role of insulin resistance in human disease. Diabetes. 1988;37:1595-607.

Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes. 1991;40:405-12.

Sabu M, Smitha K, Kuttan R. Anti-diabetic activity of green tea polyphenols and their role in reducing oxidative stress in experimental diabetes. J Ethnopharmcol. 2012;83:109-16.

Organization WH. WHO Expert Committee on Diabetes Mellitus. 1980 [meeting held in Geneva from 25 September to 1 October 1979]: second report.

Singh RP, Padmavathi B, Rao AR. Modulatory influence of Adhatoda vesica leaf extract on the enzymes of xenobiotic metabolism, antioxidant status and lipid peroxidation in mice. Mol Cell Biochem. 2000;213:99-109.

Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res. 2001;50:537-46.

Wang Z, Gleichmann H. GLUT2 in pancreatic islets: crucial target molecule in diabetes induced with multiple low doses of streptozotocin in mice. Diabetes. 1998;47:50-6.

Schnedl WJ, Ferber S, Johnson JH. STZ transport and cytotoxicity: specific enhancement in GLUT2-expressing cells. Diabetes. 1994;43:1326-33.

Thakur R, Puri HS, Husain A. Major medicinal plants of India. Central Institute of Medicinal and Aromatic Plants, Lucknow; 1989.

Asolkar L, Kakkar K, Chakre O. New Delhi: Publications and Information's Directorate, CSIR; Glossary of Indian Medicinal Plants with active principles. 1992;1:171-3.

Chatterjee RB, Das Gupta AK. Plant alkaloids VI. Berberis asiatica Roxburgh. J Indian Chem Soc. 1954;31-83.

Bhakuni D, Shoeb A, Popli S. Studies in Medicinal Plants: Part 1 - Chemical constituents of Berberis asiatica Roxb. Ind J Chem. 1968;6:123.

Dhar M, Dhawan B, Mehrotra B, Ray C. Screening of Indian plants for biological activity: Part I. Ind J Exp Bio. 1968; 6:232-47.

Kumar E, Elshurafa AA, Elango K, Subburaju T, Suresh B. Anti-tumour effect of Berberis asiatica on dalton's lymphoma ascite. Anc Sci Lif. 1998;17:290.

Chopra R, Badhwar R, Ghosh S. Poisonous plants of India. Leguminosae; 1965.

Babu T, Kuttan G, Padikkala J. Cytotoxic and anti-tumour properties of certain taxa of Umbelliferae with special reference to Centella asiatica. J Ethnopharmcol. 1995;48:53-7.

Kuttan G, Vasudevan D, Kuttan R. 55th Annual Meeting. Soc Biol Chem; 1986:105.

Mary K, Girija K, Ramadasan K. Partial purification of tumour reducing principle from Helicanthis elasticus (Fam. Loranthaceae). Can Lett. 1994;81:53-7.

Hepper FN. Old world withania (Solanaceae): a taxonomic review and key to the species. Solanaceae III Taxonomy, Chemistry, Evolution Hawkes, Kew, UK: Royal Botanic Gardens Richmond, Surrey, UK for the Linnean Society of London, London; 1991:211-28.

Maithani A, Parcha V, Pant G, Dhulia I, Kumar D. Studies on phytochemical investigation and hypoglycemic evaluation of Azadirachta indica leaves extract on alloxan induced diabetic rats. J Phar Res. 2011;4.

Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983;16:109-10.

Brosky G, Logothetopoulos J. Streptozotocin diabetes in the mouse and guinea pig. Diabetes. 1969;18:606-11.

Srinivasan K, Viswanad B, Asrat L, Kaul C, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: a model for type 2 diabetes and pharmacological screening. Pharmacol Res. 2005;52:313-20.

Joy K, Kuttan R. Anti-diabetic activity of Picrorrhiza kurroa extract. J Ethnopharmcol. 1999;67:143-8.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analyt Biochem. 1979;95:351-8.

Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Cli Med. 1963;61:882-8.

Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Bioph. 1978;186:189-95.

Claiborne A. Catalase activity. CRC handbook of methods for oxygen radical research. 1985;1:283-4.

Arulmozhi D, Veeranjaneyulu A, Bodhankar S. Neonatal streptozotocin-induced rat model of type 2 diabetes mellitus: A glance. Ind J Pharm. 2004;36:217.

Murugan P, Pari L. Antioxidant effect of tetrahydrocurcumin in streptozotocin–nicotinamide induced diabetic rats. Life Sci. 2006;79:1720-8.

Shulman GI. Cellular mechanisms of insulin resistance. J Clin Inv. 2000;106:171.

Feldman M, Friedman L, Brandt L. Sleisenger and Fordtran's gastrointestinal and liver disease. Pathophysiology/diagnosis/management. 1998;1-2:169.

Florence NT, Benoit MZ, Jonas K, Alexandra T, Désiré DDP, Pierre K, et al. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats. J Ethnopharmcol. 2014;151:784-90.

Ananthan R, Latha M, Ramkumar K, Pari L, Baskar C, Bai VN. Effect of Gymnema montanum leaves on serum and tissue lipids in alloxan diabetic rats. J Diab Res. 2003;4:183-9.

Goodarzi M, Zal F, Malakooti M, Sadeghian MSS. Inhibitory activity of flavonoids on the lens aldose reductase of healthy and diabetic rats. Acta Med Iran. 2006;44:41-5.

Sheela C, Augusti K. Antidiabetic effects of S-allyl cysteine sulphoxide isolated from garlic Allium sativum Linn. Ind J Exp Bio. 1992;30:523-6.

Whitton PD, Hems DA. Glycogen synthesis in the perfused liver of streptozotocin-diabetic rats. Biochem J. 1975;150:153-65.

Pari L, Murugan P. Effect of tetrahydrocurcumin on blood glucose, plasma insulin and hepatic key enzymes in streptozotocin induced diabetic rats. J Basic Cli Phy Pharmacol. 2005;16:257-74.

Kumar GPS, Arulselvan P, Kumar DS, Subramanian SP. Anti-diabetic activity of fruits of Terminalia chebula on streptozotocin induced diabetic rats. J Heal Sci. 2006;52:283-91.

Spence JT. Levels of translatable mRNA coding for rat liver glucokinase. J Bio Chem. 1983;258:9143-6.

Farmer EE, Davoine C. Reactive electrophile species. Curr Opi Plan Bio. 2007;10:380-6.

Aksoy N, Vural H, Sabuncu T, Aksoy S. Effects of melatonin on oxidative–antioxidative status of tissues in streptozotocin‐induced diabetic rats. Cell Biochem Func. 2003; 21:121-5.

Rauscher FM, Sanders RA, Watkins JB. Effects of coenzyme Q10 treatment on antioxidant pathways in normal and streptozotocin‐induced diabetic rats. J Biochem Mol Toxicol. 2001;15:41-6.

Downloads

Published

2017-07-22

How to Cite

Khan, M. M. H., Rastogi, C., Gupta, S., Paswan, S. K., Verma, P., Jawaid, T., & Rao, C. V. (2017). Protective effect of Berberis asiatica root on biochemical and histopathological changes in streptozotocin-induced diabetic Wistar rats. International Journal of Basic & Clinical Pharmacology, 6(8), 1880–1889. https://doi.org/10.18203/2319-2003.ijbcp20173110

Issue

Section

Original Research Articles