DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20173290

Raw turmeric and pure curcumin: a comparison of embryonic cytotoxicity in zebrafish

Raskin Erusan Rajagopal, Meenakshi Balasubramanian, Shantaraman Kalyanaraman

Abstract


Background: Turmeric (Curcuma longa) is widely used in ayurveda and siddha formulations for treating various ailments. This study compares the gross effects of curcumin and natural state turmeric on zebrafish embryos.

Methods: Turmeric powder and curcumin were used in this study. Zebrafish embryos were treated with different concentration of both turmeric and curcumin to evaluate their gross effects including toxicity. Morphological variations including hatching, survival, heart rates, head width, eye width, tail width and yolk sac area were examined by stereomicroscope.

Results: Embryos treated with curcumin or raw turmeric with concentration of 27.628 and 36.83µg/ml exhibited a reduction of survival rates (15-20%) at 24, 48 and 72hpf. Turmeric treated embryos had near normal hatching rates when compared to curcumin treated embryos at 48hpf whereas decreased hatching rate was observed in turmeric treated groups at 72hpf when compared to curcumin treated group. No significant morphological anomaly in head /eye / tail width, length of yolk sac and average embryo length were witnessed among all the 3 groups. The toxicity assays revealed various morphological deformities in embryos treated with different concentrations of both turmeric and curcumin.

Conclusions: Zebrafish embryos treated with increasing concentrations of curcumin in pure form or raw turmeric exhibited similar morphometric and toxic effects indicating that raw turmeric could elicit anti cancer activity with similar potency as that of active ingredient curcumin due to their toxicity attribute. It may be beneficial to explore the negative attributes of such common natural food ingredients that may be beneficial for therapeutic interventions.

 


Keywords


Curcumin, Raw turmeric, Toxicity, Zebrafish

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References


Priyadarsini K. The chemistry of curcumin: from extraction to therapeutic agent. Molecules. 2014;19(12):20091-112.

Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric and curcumin: Biological actions and medicinal applications. Current Science. 2004;87(1):44-53.

Ragasa C, Laguardia M, Rideout J. Antimicrobial sesquiterpenoids and diarylheptanoid from Curcuma domestica. ACGC Chem Res Comm. 2005;18(1):21-4.

Mishra S, Palanivelu K. The effect of curcumin (turmeric) on Alzheimer's disease: An overview. Ann Indian Acad Neurol. 2008;11(1):13-9.

Karpaga M, Sridhar SN, Swaminathan MGRP, Sripradha R. Efficacy of turmeric as adjuvant therapy in type 2 diabetic patients. Indian Journal of Clinical Biochemistry. 2014;30(2):180-6.

Watson RR. Foods and Dietary Supplements in the Prevention and Treatment of Disease in Older Adults. 1st Ed. Academic Press; 2015.

Shiyou Li, Wei Y, Guangrui D, Ping W, Peiying Y, Bharat B. Aggarwal. Chemical Composition and Product Quality Control of Turmeric (Curcuma longa L.) Pharmaceutical Crops. 2011;2:28-54,2210-906.

Kaur J, Chawla R. Food Can Be Medicine - Medicine Can Be Food. By Educreation Publishing, 31-Oct-2015.

Tayyem RF, Heath DD, Al-Delaimy WK, Rock CL. Curcumin content of turmeric and curry powders. Nutr Cancer. 2006;55(2):126-31.

Reason W, Mysore SV, Marilene BW, Eri SS. Curcumin: A review of anti-cancer properties and therapeutic activity in head and neck squamous cell carcinoma, Mol Cancer. 2011;10:12.

Joe B, Vijaykumar M, Lokesh BR. Biological properties of curcumin- cellular and molecular mechanisms of action. Crit Rev Food Sci Nut. 2004;44:97-111.

Siwak DR, Shishodia S, Aggarwal BB, Kuzrock R. Curcumin-induced antiproliferative and proapoptotic effects in melanoma cells are associated with suppression of Ikappa B kinase and nuclear factor kappa B activity and are independent of the B-Raf/mitogen-activated/extracellular signal-regulated protein kinase pathway and the Akt pathway. Cancer. 2005;104:879-90.

Aggarwal S, Takada Y, Singh S, Myers JN, Aggarwal BB. Inhibition of growth and survival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor-kB signaling. Int J Cancer. 2004;111:679-92.

Mehta K, Pantazis P, McQueen T, Aggarwal BB. Antiproliferative effect of curcumin (diferuloylmethane) against human breast tumor cell lines. Anticancer Drugs. 1997;8:470-81.

Hanif R, Qiao L, Schiff SJ, Rigas B. Curcumin, a natural plant phenolic food additive, inhibits cell proliferation and induces cell cycle changes in colon adenocarcinoma cell lines by a prostaglandin-independent pathway. J Lab Clin Med. 1997;130:576-84.

Kanai M. Therapeutic applications of curcumin for patients with pancreatic cancer. World J. Gastroenterol. 2014;20:9384-91.

Mukhopadhyay A, Bueso-Ramos C, Chatterjee D, Pantazis P, Aggarwal BB. Curcumin downregulates cell survival mechanisms in human prostate cancer cell lines. Oncogene. 2001;20:7597-609.

Lin YG, Kunnumakkara AB, Nair A, Merritt WM, Han LY, Armaiz-Pena GN, et al. Curcumin inhibits tumor growth and angiogenesis in ovarian carcinoma by targeting the nuclear factor-κB pathway. Clinical Cancer Research. 2007 Jun 1;13(11):3423-30.

Mohandas KM, Desai DC. Epidemiology of digestive tract cancers in India. V. Large and small bowel. Indian J Gastroenterol. 1999;18:118-21.

Oda Y. Inhibitory effect of curcumin on SOS functions induced by UV irradiation. Mutat Res. 1995;348:67-73.

Thapliyal R, Maru GB. Inhibition of cytochrome p450 isoenzymes by curcumins in vitro and in vivo. Food Chem Toxicol. 2001;39:541-7.

Iqbal M, Sharma SD, Okazaki Y, Fujisawa M, Okada S. Dietary supplementation of curcumin enhances antioxidant and phase II metabolizing enzymes in ddY male mice: possible role in protection against chemical carcinogenesis and toxicity. Pharmacol Toxicol. 2003;92:33-8.

Krishnaswamy K, Goud VK, Sesikeran B, Mukundan MA, Krishna TP. Retardation of experimental tumorigenesis and reduction in DNA adducts by turmeric and curcumin. Nutr Cancer. 1998;30:163-6.

Inano H, Onoda M, Inafuku N, Kubota M, Kamada Y, Osawa T, et al. Chemoprevention by curcumin during the promotion stage of tumorigenesis of mammary gland in rats irradiated with gamma-rays. Carcinogenesis.1999;20:1011-8.

Collett GP, Robson CN, Mathers JC, Campbell FC. Curcumin modifies Apc (min) apoptosis resistance and inhibits 2-amino 1-methyl-6-Phenylimidazo [4,5-bpyridine (PhIP) induced tumour formation in Apc (min) mice. Carcinogenesis. 2001;22:821-5.

Westerfield M. The Zebrafish book. A Guide for the laboratory use of Zebrafish Danio rerio. Eugene: University of Origon Press; 1989.

Bakkiyanathan A, Joseph AM, Tharani L, Malathi R. Genistein, the phytoestrogen induces heart-and-soul (has) phenotypes in zebrafish embryo. J. Dev. Biol. Tissue. Eng. 2010;2(2):18-22.

Nathan JR, Lakshmanan G, Michael FM, Seppan P, Malathi R. Expression of adenosine receptors and vegf during angiogenesis and its inhibition by pentoxifylline- A study using zebrafish model. Biomed Pharmacother. 2016;84:1406-18.

Zhang Z, Hu J, Zhen H, Wu X, Huang C. Reproductive inhibition and transgenerational toxicity of triphenyltin on medaka (Oryziaslatipes) at environmentally relevant levels. Environ Sci Technol. 2008;42:813-39.