DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20172634

Analgesic and anti-inflammatory activity of ethanolic extracts of leaf and fruit pulp of Aegle marmelos in albino rats

Krishnakanth K., Abhilash B. G., Neeraja K., Hari Jagannadha Rao G., Chakrapani Cheekavolu

Abstract


Background: To study the analgesic and anti-inflammatory activity of the ethanolic extracts of leaf (LE) and fruit pulp (FE) of Aegle marmelos in comparison with the standard drugs pentazocine and diclofenac in albino rats. 56 healthy wistar albino rats of either sex were randomly divided into 14 groups of 4 each (n=4), weighing about 150-200grams were selected for the study. All drugs i.e. 1ml of 1% Carboxy methyl cellulose (CMC), pentazocine 10mg/kg (intraperitoneal), diclofenac 10 mg/kg and LE and FE at doses of 100mg/kg and 200mg/kg in 1% CMC (vehicle) were administered per oral one hour before the experiment.

Methods: Analgesic activity was assessed by Eddy’s hot plate method (latency time) and acetic acid induced writhing (number of writhings) method. Anti-inflammatory activity was assessed by carrageenan induced hind paw edema method using digital plethysmometer.

Results: Test drugs at doses LE 200mg/kg and FE 200mg/kg have shown significant increase in mean latency time in Eddy’s hot plate (P <0.001) and decrease in the average number of writhing movements (P <0.01). LE 200 and FE 200 doses did not show significant anti-inflammatory activity in Carrageenan induced paw edema model (P >0.05).

Conclusions: Leaf extract (LE) and fruit extract (FE) at doses 200mg/kg have shown significant analgesic action and no anti-inflammatory action.


Keywords


Analgesic, Anti-inflammatory, Aegle marmelos

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References


Muralidhar A, Sudhakar BK, Ravi Sankar T, Reddanna P, Reddy GV, Latha J. Anti-inflammatory activity of flavanoid fraction isolated from the stem bark of Butea monosperma (LAM): A mechanism based study. Int J Phytopharmacol 2010;1(2):124.

Pravin VG, Priti SS, Sayyed N, Amol BC. Development and evaluation of analgesic polyherbal formulation containing some indigenous medicinal plants. Int J Pharm and Bio Sci Sep. 2011;2(3):120.

Zulfiker AHM, Mahbubur RM, Kamal HM, Hamid K, Mazumder MEH, Sohel RM. In vivo analgesic activity of ethanolic extracts of two medicinal plants - Scoparia dulcis L. and Ficus racemosa Linn. Biol and Med. 2010;2(2):42.

Patsnaik AK, Kodati D, Pareta SK, Patra KC, Harwansh RK. Analgesic and anti-inflammatory activities of Buchanania lanzan spreg. Roots. Res J Pharm Biol and Chem Sci. Mar 2011;2(1):420.

Aswatha ram HN. Herbal formulations and their standardization. In: Trivedi PC editors. Medicinal plants traditional knowledge. 1st Ed. India: I.K International Pvt Ltd; 2006:1-2.

Saroj K, Manish M, Tonpay SD. Anxiolytic and antidepressant activities of methanolic extract of Aegle marmelos leaves in mice. Ind J Physiol Pharmacol. 2010;54(4):319.

Wahid AM, Suyog DM, Suraj BJ, Ajinkya MP, Mukhtar SK, Madhukar RV. Evaluation of anti-inflammatory and analgesic activities of ethanolic extract of roots Adathoda vasica linn. Int J Pharm Res. Apr-Jun 2010;2(2):1364-68.

Lokesh TN. Analgesic activity of aqueous and alcohol root extracts of Pergularia daemia (forsk) chiov. Int J Pharm Pharm Sci. Nov-Dec 2009;1(1):33-7.

Amberker MV, Tara S, Kumari MK, Bairy KL, Smitha S. Evaluation of anti-inflammatory and analgesic activities of alcoholic extract of Kaempferia galanga in rats. Ind J Physiol Pharmacol. 2011;55(1):13-24.

Verma R, Purohit S, Bhandari A, Priyanka P. Analgesic and anti-inflammatory activities of ethyl acetate extract of leaves of Salvadora persica l. Int J Pharm Biol Arch. 2010;1(1):51-5.

Mayur RB, Yogesh MB, Rohan UW, Umesh TW, Pankaj SS. The analgesic activity of ethanolic extract of tubers of Remusatia vivipara roxb. J Pharmacol Toxicol. 2011;1(6):61-6.

Anthony JA, Oluseyi MA, Adeolu AA, Margaret OS. Anti-inflammatory and analgesic activity of the methanol extract of Malva parviflora linn (malvaceae) in rats. Afr J Biotech. Feb 2010;9(8):1225-29.

Sharma A, Bhatia S, Kharya MD, Gajbhiye V, Ganesh N, Namdeo AG, et al. Anti-inflammatory and analgesic activity of different fractions of Boswellia serrata. Int J Phyto. 2010;2:94-9.

Raghunadha RS, Rajini P, Koteswararao D, Naidu YK, Chandiran SI, Reddy KMP. Anti-inflammatory and analgesic activity of Smilax chinensis. Res J Pharm, Biol And Chem Sci. Jun 2010;1(2):1-2.

Arul V, Miyazaki S, Dhananjayan R. Studies on the Anti-inflammatory, Anti pyretic and Analgesic properties of the leaves of Aegle marmelos corr. J Ethnopharmacol. 2005;96:159-63.

Saroj K, Manish M, Tonpay SD. Anxiolytic and antidepressant activities of methanolic extract of Aegle marmelos leaves in mice. Ind J Physiol Pharmacol. 2010;54(4):319.

Rao ChV, Ojha SKA, Mehrotra S, Pushpangadan P. Analgesic, anti-inflammatory and antiulcerogenic activity of the unripe fruits of Aegle marmelos. Acta Pharm Tur. 2003;45:85-91.

Agung EN, Sugeng R, Mohamad AS, Kazutaka M. Anti-allergic effects of Marmin, a coumarine isolated from Aegle marmelos Correa: In vitro study. Int J Phytomedicine. 2011;3:84-97.

Achyut NK, Rajesh KG, Santosh KS, Sandhya D, Geeta W. Hypoglycemic and antihyperglycemic activity of Aegle marmelos seed extract in normal and diabetic rats. J Ethnopharmacol. 2006;107:374-79.

Krushna G, Kareem MA, Devi KL. Antidyslipidaemic effect of Aegle marmelos Linn. Fruit on Isoproterenol induced myocardial injury in rats. The Internet J Pharmacol. 2009;6(2):1-6.

Maity P, Hansda D, Bandyopadhyay U, Mishra DK. Biological activities of crude extracts and chemical constituents of Aegle marmelos corr. Ind J Exp Bio. 2009;47:849-61.

Rajesh K, Ashok K, Chandra Shekhar P. Insecticidal activity of Aegle marmelos (L.) Correa essential oil against four stored grain insect pests. Internet J Food Safety. 2008;10:39-49.

Rana BK, Singh UP, Taneja V. Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos. J Ethnopharmacol. Elsevier. 1997;57:29-34.

Meshram M, Ravikanth K, Maini S, Rekhe DS. Treatment of clinical cases of Bacterial enteritis in Goat with new polyherbal antidiarrhoeal formulation. Veterniary World. Apr 2009;2(4):143-5.

Lambole VB, Krishna Murti, Upendra K, Bhatt SKP, Gajera V. Phytopharmacological properties of Aegle marmelos as a potential medicinal tree. Int J Pharm Sci Rev and Res. 2010;5(2):67-72.

Ankur C, Aadarsh C, Asish M, Shilpi M, Patil UK. Evaluation of the Immunomodulatory activity of methanolic and ethanolic extract of leaves of Aegle marmelos in rats. Int J Drug Dev and Res. Jul-Sep 2010;2(3):664-8.

Alka C, Meera A, Sonalika K, Anju M. Suppression of fertility in male albino rats following the administration of 50% ethanolic extract of Aegle marmelos. Contraception. 2007;76:474-81.

Rishabha M, Kumar A, Singh A, Kulkarni GT. Pharmacological screening, ayurvedic values and commercial utility of Aegle marmelos. Int J Drug Dev Res. Jan-Mar 2012;4(1):28-37.

Shankarnanth V, Balakrishnan N, Suresh D, Sureshpandian G, Edwin E, Sheeja E. Analgesic activity of methanol extract of Aegle marmelos leaves. Biological Trace Element Research. 2007;78:258-59.

Gautam MK, Ghatule RR, Singh A, Purohit V, Gangwar M. Ind J Exp Biol. Feb 2013;51:157-64.

Neto AG, Costa JMLC, Belati CC. Analgesic and anti-inflammatory activity of crude root extract of Pfaffia glomerata (Spreng) Pedersen. J Ethnopharmacol. 2005;96:87-91.