Investigation of anti-arthritic potential of Acacia torta (roxb.) craib
Keywords:Acacia Torta (Roxb.) Craib, Anti-arthritic, Freund’s Adjuvant, Indomethacin, Paw edema, Prostaglandin
Background: Acacia torta (Roxb.) Craib, traditionally used in respiratory, circulatory, digestive, musculo-skeletal, skin diseases etc., Numerous plants of this genus have been used in folk medicine to treat stomach pain, cough, diarrhoea, piles, sore-throat; as astringent, antipyretic, antimicrobial, antimalarial, antiviral, anti-oxidant and as anti-hypertensive. Hence, the present study was aimed to investigate the anti‐arthritic potential of Acacia Torta (Roxb.) Craib, since, systematic study of this plant has not been carried out.
Methods: Ethanol and water were used to prepare stem extract by soxhlet extraction method. The extract is evaluated by using Freund’s complete adjuvant induced arthritis in albino wistar rats at the dose of 100mg/kg/p.o. and 400mg/kg/p.o. and indomethacin at 10mg/kg were used for the study.
Results: The hydro alcoholic extract of the stem Acacia torta (Roxb.) Craib in crude form contains phytoconstituents like saponins, tannins, steroids, and triterpenes. The reference standard, Indomethacin at the dose of 10 mg/kg showed significant inhibition of paw edema by 71.61% (P <0.001), as compared to the vehicle control. Treatment with the hydro alcoholic extract of stem Acacia Torta at doses of 100 and 400 mg/kg caused significant inhibition of paw edema by 59.26% (P <0.001) and 69.14% (P <0.001) respectively, was observed on 21st day, as compared to the vehicle control.
Conclusions: The data obtained from the present study indicated that several factors may contribute to the anti-inflammatory and anti-arthritic activity of hydro alcohol extract of the stem Acacia torta (Roxb.) Craib. adjuvant induced arthritis showed inhibition of prostaglandin synthesis might be the major mechanism by which the hydro alcoholic extract of the stem Acacia torta (Roxb.) Craib exerts anti-inflammatory and arthritic activity.
Meyer DM, Jesson MI, Li X, Elrick MM, Shippy CLF, Warner JD, et al. Anti-inflammatory activity and neutrophil reductions mediated by the JAK1/JAK3 inhibitor, CP-690,550 in rat adjuvant-induced arthritis, Journal of Inflammation. 2010;7(41):112-6.
Bang JS, Oh DH, Choi HM. Anti-inflammatory and antiarthritic effects of piperine in human interleukin 1-stimulated fibroblast-like synoviocytes and in rat arthritis models. Arthritis Research and Therapy. 2009;11(2):1-9.
Bansod MS, Kagathara VG, Pujari RR, Patel VB, Ardeshna HH. Therapeutic effect of a poly-herbal preparation on adjuvant induced arthritis in wistar rats, International Journal of Pharmacy and Pharmaceutical Sciences. 2011;3(2):186-92.
Ahmed S, Anuutiyo J, Malemud CJ, Haqqi TM. Biological basis for the use of botanicals in osteoarthritis and rheumatoid arthritis: a review, Evidence-Based Complementary and Alternative Medicine. 2005;2(1):301-8.
Thite AT, Patil R, Naik SR. Anti-arthritic activity profile of methanolic extract of Ficus bengalensis: Comparison with some clinically effective drugs, Biomedicine & Aging Pathology. 2014;3(4):207-17.
Koli C, Akah PA, Nwafor S. Anti-inflammatory activity of plants, Journal of Natural Remedies. 2003;3(1):1-30.
Singh S, Majumder DK. Effect of fixed oil of Ocimum sanctum against experimentally induced arthritis and joint edema in laboratory animals, Pharmaceutical Biology. 1996;34(3):218-22.
Bokhad MN, Rothe SP. An Overview of Medicinally Important Lianas from Dry Deciduous Forest of West vidarbha Region (M.S) India. Bioscience Discovery. 2015;6(2):117-20.
Girish N, Kumar AKV, Nagaraju B. Evaluation of anti-inflammatory activity of Acacia torta (Roxb.) Craib. International Journal of Experimental Pharmacology. 2017;7(2):39-42.
Girish N, Kumar AKV, Nagaraju B. Screening of anti-inflammatory activity of Acacia torta (Roxb.) Craib using cotton pellet induced granuloma. International Journal of Preclinical and Pharmaceutical Research. 2017;8(2):40-3.
Mohammad R, Shariq S, Roohi Z, Malik I. Bark of Acacia Arabica-A Nature’s Gift: An Overview. Int. Res J. Med Sci. 2014;2(5):20-4.
OECD/OCDE 423-OECD guideline for testing of chemicals-Acute Oral Toxicity; Acute Toxic Class Method. Adopted: 17th December 2001. Available at: https://ntp.niehs.nih.gov/iccvam/suppdocs/feddocs/oecd/oecd_gl423.pdf
Jubie S, Jawahar N, Koshy R, Gowramma B, Murugan V, Suresh B. Anti-arthritic activity of bark extracts of Alangiumsalviifoliumwang. Rasayan J. Chem. 2008;1:433-6.
Rajaram C, Reddy RK, Chandra Sekhar K. Evaluation of anti-arthritic activity of Caesalpiniapulcherrimain freund’s complete adjuvant induced arthritic rat model. J. Young Pharmacists. 2015;7(2).
Chatterjee S, Das SN. Anti-inflammatory effect of a poly-herbal drug & its mechanism of action. Indian J. Pharmacol. 1996;28:116-9.
Begum VH, Sadique J. Long term effect of herbal drug Withaniasomnifera on adjuvant induced arthritis in rats. Indian J Exp Biol. 1988;26:877-82.
Watnick AS, Sabin C. The effect of clonixin and betamethasone on adjuvant induced arthritis and experimental allergic encephalomyelitis in rats. Japan J. Pharmacol. 1972;22:741-8.
Crofford LJ, Sano H, Karalis K, Webster EL, Goldmuntz EA, Chrousos GP, et al. Local secretion of corticotropin-releasing hormone in the joints of Lewis rats with inflammatory arthritis. J. Clin. Invest. 1992;90:2555-64.
Vijayalakshmi T, Muthulakshmi V, Sachdanandam P. Salubrious effect of Semecarpusanacardium against lipid peroxidative changes in adjuvant arthritis studied in rats. Mol. Cell Biochem. 1997;175:65-9.
Gureyev TE, Stevenson AW, Paganin D, Mayo SC, Pogany A. Quantitative methods in phasecontrast X-ray imaging. J Digit Imaging. 2000;13:121-6.