Evaluation of pharmacological and toxicological studies of an ayurvedic medicine Rasaraj Ras on biological system of the male Sprague-Dawley rats
DOI:
https://doi.org/10.18203/2319-2003.ijbcp20164090Keywords:
Absolute weight, Organ percent weight, Organ water content Pharmacological, Rasaraj Ras, ToxicologicalAbstract
Background: In this study, the pharmacological and toxicological effects along with possible side effects of the classical ayurvedic formulation Rasaraj Ras (RR) which is used as a traditional medicine in the treatment of hemiplegia in the rural population were evaluated.
Methods: During this study, various experiments on body growth rate, organ-body weight ratio and tissue hydration indices were performed to evaluate its efficacy and toxicity. To find out the toxicological characteristic of RR, it was administered chronically to the male Sprague-Dawley rats at a dose of 40 mg/kg. After 28 days chronic administration of the RR preparation the following toxicological changes were noted.
Results: All throughout the experimental period the RR treated animals were always maintaining negligible changes in body weight, but all throughout the experimental period no statistically significant increase or decrease was noted. There is a statistically significant decrease in the relative percent weight of the male rat heart. There is a statistically highly significant decrease in the absolute weight of the male rat liver. There is a statistically highly significant decrease in the relative percent weight of the male rat liver. There is a statistically significant decrease in the relative percent weight of the male rat kidney. In the tissue hydration index determination, no significant changes were noted in case of any organ.
Conclusions: As RR decreases abnormally weight of several organs in body of treated rats, so it should not be administered chronically at a higher dose.
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References
WHO. Regional Office for the Western Pacific Seminar on the Use of Medicinal Plants in Health Care, Final Report, Tokyo, Japan: 1977, pp. 13-7.
WHO. WHO Launches the First Global Strategy on Traditional and Alternative Medicine, Press Release, WHO / 38: 2002.
WHO. Consultation Meeting on Traditional Medicine and Modern Medicine: Harmonizing the Two Approaches. Geneva, World Health Organization, (document reference (WP) TM/ICP/TM/001/RB/98–RS/99/GE/32(CHN), Geneva, Switzerland: 1999a.
WHO. Traditional, Complementary and Alternative Medicines and Therapies. Washington DC, WHO Regional Office for the Americas/Pan American Health Organization (Working group OPS/OMS), Washington DC, USA: 1999b.
Ernst E. Pharmacoepidemiol Drug Saf. 2002;11(6):455-6.
Anonymous. Bangladesh National Formulary of Ayurvedic Medicine 1992 (Approved by the Government of Bangladesh vide Ministry of Health and Family Welfare Memo No. Health-1/Unani-2/89/(Part-1) 116 dated 3-6-1991). National Unani and Ayurvedic Formulary Committee Bangladesh Board of Unani and Ayurvedic Systems of Medicine, 38, Bangabandhu Avenue, Dhaka-1000. Second ed. 2011.
Anonymous, The Ayurvedic formulary of India, Part I. 151 edition New Delhi: Government of India, Ministry of health and family welfare, Department of health. 1978; pp. 249.
OECD Guideline (425) for the testing of chemicals, Guidance document on acute oral toxicity, Environmental Health and Safety Monograph Series on Testing and Assessment, 2000.
Gad SC. An Approach to the Design and Analysis of Screening Studies in Toxicology, Intl J Tox. 1988;7:(2):127-38.
Stevens KR, Gallo MA. Practical consideration in the conduct of chronic toxicity studies, Principles and Methods of Toxicology, 2nd edn. Chap. VIII, 1989.
Ringler H, Dabich L. Hematology and clinical biochemistry. In: The Laboratory Rat Biology and Disease [Baker HL ed].American College of Laboratory Animal Medicine Series Academic Press, 1979.
Saad B, Azaizeh H, Abu-Hijleh G, Said S. Evid. Based Complement. Alternat. Med. 2006;3:433-9.
Haschek WM, Rousseaux CG, Wallig MA. Haschek and Rousseaux's Handbook of Toxicologic Pathology 3rd revised ed., Chapter 6, Elsevier Science: pp. 154. 2013.
Dybing E, Doe J, Groten J, Kleiner J, Brien J. Food Chem Toxicol. 2002;42:237-82.
Amresh GR, Singh PN, Rao VC. J. Ethnopharmacol. 2008;116:454-60.
Haschek and Rousseaux's Handbook of Toxicologic Pathology. Ed W.M. Haschek, C.G. Rousseaux and M.A. Wallig. 3rd revised ed., Elsevier Science, 2013 ISBN 9780124157651. Chapter 7 pp. 271.
Spokas EG, Suleymanov OD, Bittner SE. Cardiovascular effects ofchronic high-dose atriopeptin III infusion in normotensive rats. Toxicology and Applied Pharmacology. 1987;91:305-14.
Schmucker DL. Age-related changes in liver structure and function: implications for disease. Experimental Gerontology. 2005;40: 650-9.
Muhlberg W, Piatt D. Age-dependent changes of the kidneys: pharmacological implications. Gerontology. 1999;45:243-53.
