Screening of alcohol de-addiction potential of zinc: a study on albino rats


  • Priya Gandigawad Department of Pharmacology, SDM Medical College, Dharwad, Karnataka, India
  • Sharanabasayyaswamy B. Hiremath Department of Pharmacology, Kodagu Institute of Medical Sciences, Madikeri, Karnataka, India



Alcohol de-addiction, Topiramate, Zinc sulfate


Background: With regard to tobacco and alcohol addiction, involvement of NMDA receptors and glutamate as neurotransmitter has been highlighted. Few indirect evidences support possible de-addiction potential of zinc being mediated through their interaction with nicotinic and NMDA receptors. The objectives of the present study were planned to analyze the alcohol de-addiction potential of oral zinc sulfate as a monotherapy and as a supplement to topiramate, in male wistar albino rats.

Methods: Male wistar albino rats were exposed to ‘two-bottle free choice’ model of voluntary ethanol consumption, wherein each animal had access to water and 10% v/v ethyl alcohol in two separate bottles. Thirty male rats divided into five groups, each containing six rats were treated as control group, receiving 1ml/kg/d distilled water orally; zinc sulfate group receiving 18mg/kg/d of elemental zinc orally; topiramate group receiving topiramate 5mg/kg/d, orally and Topiaramate plus Zinc sulfate group receiving both zinc sulfate (18mg/kg/d) and topiramate (5mg/kg/d) orally for two weeks.

Results: There were no significant differences with regard to all the three parameters in two-way ANOVA. However, there were significant differences with regard to amount of alcohol and water consumed but not API, in all the treatment groups in post-hoc test.

Conclusions: It is unclear on to the alcohol de-addiction potential of zinc as monotherapy and combination therapy with topiramate.


Ali SF, Onaivi ES, Dodd PR, Cadet JL, Schenk S, Kuhar MJ, et al. Understanding the Global Problem of Drug Addiction is a Challenge for IDARS Scientists. Curr Neuropharmacol. 2011 Mar;9(1):2-7. Available at:

Pratima M, Manjunatha N, Subodh BN, Prabhat KC, Vivek B. Substance use and addiction research in India. Indian J Psychiatry. 2010;52:S189-99. Available at:

The addiction recovery guide. Your online guide to drug and alcohol ddiction recovery. 2017. Available at: Accessed on 19 May 2017.

Safai-Kutti S. Oral zinc supplementation in anorexia nervosa. Acta Psychiatr Scand. 1990;361:14-7. Available at:

Joshi M, Akhtar M, Najmi AK, Khuroo AH, Goswami D. Effect of zinc in animal models of anxiety, depression and psychosis. Hum Exp Toxicol. 2012;31:1237-43. Available at:

Du J, Zhu M, Bao H, Li B, Dong Y, Xiao C, et al. The Role of Nutrients in Protecting Mitochondrial Function and Neurotransmitter Signaling: Implications for the Treatment of Depression, PTSD, and Suicidal Behaviors. Crit Rev Food Sci Nutr. 2014;3:0. Available at:

Katalin T. Zinc in Neurotransmission. Annual Review of Nutrition. 2011;31:139-53. Available at:

Nechifor M. Magnesium and Zinc Involvement in Tobacco Addiction. J Addict Res Ther. 2012;S2:005. Available at:

Prior LP, Galduróz JC. Glutamatergic hyperfunctioning during alcohol withdrawal syndrome: Therapeutic perspective with zinc and magnesium. Medical Hypotheses. 2011;77:368-70. Available at:

Arbaizar B, Dierssen-Sotos T, Gomez-Acebo I, Llorca J. Topiramate in the treatment of alcohol dependence: a meta-analysis. Actas Espanolas de Psiquiatria. 2010;38(1):8-12. Available at:

Morud J, Adermark L, Ericson M, Söderpalm B. Alterations in ethanol-induced accumbal transmission after acute and long-term zinc depletion. Addict Biol. 2015;20(1):170-81. Available at:

McCracken LM, Trudell JR, Goldstein BE, Harris RA, Mihic SJ. Zinc enhances ethanol modulation of the alpha1 glycine receptor. Neuropharmacology 2010;58(3):676-81. Available at:

McCracken LM, Trudell JR, McCracken ML, Harris RA. Zinc-dependent modulation of α2- and α3-glycine receptor subunits by ethanol. Alcohol Clin Exp Res. 2013;37(12):2002-10. Available at:

McCracken LM, Blednov YA, Trudell JR, Benavidez JM, Betz H, Harris RA. Mutation of a zinc-binding residue in the glycine receptor α1 subunit changes ethanol sensitivity in vitro and alcohol consumption in vivo. J Pharmacol Exp Ther. 2013;344(2):489-500. Available at:

Kirson D, Cornelison GL, Philpo AE, Todorovic J, Mihic SJ. Physiological concentrations of zinc reduce taurine-activated GlyR responses to drugs of abuse. Neuropharmacology. 2013;75:286-94. Available at:

Medscape news and perspectives. Topiramate in the Treatment of Alcohol Use Disorders 2015. Available at: Accessed 19 May 2017.




How to Cite

Gandigawad, P., & Hiremath, S. B. (2017). Screening of alcohol de-addiction potential of zinc: a study on albino rats. International Journal of Basic & Clinical Pharmacology, 6(8), 1977–1980.



Original Research Articles