Effect of Sesbania grandiflora Linn leaf extracts on diuresis in wistar rats
Keywords:Aqueous and methanolic extract, Diuresis, Sesbania grandiflora Linn
Background: This study was designed to evaluate the effect of sesbania grandiflora linn leaf extracts on diuretic activity in wistar rats.
Methods: Healthy male wistar albino rats divided into 9 groups and weighing 150-200gm.diuretic activity was expressed by Natriuretic activity, saluretic activity, carbonic anhydrase inhibition, Diuretic index or Diuretic action, saluretic index, Natriuretic index. Animalgroups are divided into: group1 treated as control, 2and 3 treated as standards, group 4, 5 and 6 treated with aqueous leaf extract of Sesbania grandiflora Linn. in different doses of 150,300,450mg/kg. Group 7,8and 9 treated with methanolic leaf extract of Sesbania grandiflora Linn. Doses of 150,300,450mg/kg.
Results: No change in body weight group treated with AE-150 (p>0.05) with AE-150 (0.1%), increase in urine volume with increasing doses both with aqueous and methanolic extracts, sodium excretion with methanolic extract was high compared with the aqueous extract, significant increase in potassium excretion in both aqueous and methanolic extract treated groups.All the statistical tests were done by using SPSS version 6. The level of significance was 5% (p≤0.05).
Conclusions: All the electrolytes concentration was increased in the urine, hence excluding osmotic diuretic action. Diuretic index, saluretic activity, saluretic index and natriuretic index of ME-450 were more than the value of hydrochlorothiazide hence excludes thiazide diuretic action. But these values were less than furosemide.
Brunton LL, Chabner BA, Knollmann BC. Goodman and Gilman’s: The pharmacological basis of therapeutics. 12th Ed. New Delhi: Mc Graw Hill publication; 2011:677.
Dubey S, Verma VK, Sahu AK, Jain AK, Tiwari A. Evaluation of diuretic activity of aqueous and alcoholic rhizomes extracts of Costus speciosus Linn in wistar albino rats. Int J Res Ayurveda Pharm. 2010;1(2):648-52.
Uddin SM, Misra V, Banerjee S. Laxative and diuretic property of ethanolic extract of leaves of Alocasia macrorrhiza Linn on experimental albino rats. Int Res J Pharm. 2012;3(2):174-6.
Barrett KE, Barman SM, Boitano S, Brooks HL. Ganong’s review of medical physiology. 24th ed. New Delhi: Tata Mc Graw Hill Lange; 2012:671.
Vaishali M, Deorao A, Pallavi V, Aditi K, Utkarsh B, Rachana S. Evaluation of diuretic activity of aqueous extract of Raphanus sativus. Eur J Biol Sci. 2011;3(1):13-5.
Mondal S, Parhi R, Suresh P, Dash GK. Studies on diuretic and laxative activity of Acacia suma (Roxb) barks. Int J Res Ayurveda Pharm. 2010;1(2):510-4.
Umesh KS, Uma SS, Abhishek S, Vijit A. Diuretic activity of Kigelia pinnata bark extract. J Pharmacol Res. 2010;1(2):17-20.
Shivalinge GKP, Satish S, Mahesh CM, Vijaykumar. Study on the diuretic activity of Cynodon dactylon root stalk extract in albino rats. Res J Pharm Tech. 2009;2(2):338-40.
Kumarasamyraja D, Shankar M, Gowrishankar NL. Preliminary phytochemical and diuretic potential of methanolic extract of Azima tetra cahntha lam, leaf. Int J Pharm Indian Res. 2011;1(4):275-8.
Shreedevi MS. Acute toxicity and diuretic activity of Mangifera indica bark extracts. Int J Pharm Bio Sci. 2011;2(3):141-6.
Diuretic activity. In: vogel HG editors. Drug discovery and evaluation: pharmacological assays. 3rd Ed. Germany: Springer-verlag; 2008:459-460.
Hazem AK, Lilaibrahium AB, Omayma EG, Abeer T. Diuretic activity of alcoholic extract of Tribulus alatus in rats. J Pharm Res. 2009;2(5):792-4.
Swapnadeep P, Jain DC, Joshi SB. Diuretic activity of the extracts of Limonia acidissima in rats. Rasayan J Chem. 2009;2(1):53-6.
Biradar K, Khavane K, Payghan S, Ramchandra SS. Evaluation of diuretic activity of Phyllanthus fraternus web arial parts on albino rats. Int J Pharm Biol Arch. 2010;1(4):389-92.
Rammohan M, Pandu R, Srinivas RC. Comparative diuretic activity of seed and fruit wall extract of Solanum torvum. J Drugs Med. 2011;3(1):50-3.
Rejitha G, Anbu JJS, Suraj R, Amitava D, Kiran N. Diuretic activity of Eupatorium odoratum Linn. J Pharma Res. 2009;2(5):844-6.
Eswaraiah MC, Elumalai A, Nikhitha M, Areefa S, Mamatha A, Usha et al. Evaluation of Diuretic Activity of Aqueous and Methanol Extracts of Sesbania grandiflora Linn in Rats. Int J Pharm Tech Res. 2012;4(2):835-8.
Rajasekaran A, Murugesan S. Diuretic, CNS depressant and laxative evaluation of the leaf extract of Sesbania grandiflora. Int Chem Sci. 2003;1(4):436-9.
Sahoo HB, Asati AK, Toppo FA, Kori ML. Evaluation of polyherbal formulation for diuretic activity in albino rats. Asian Pac J Trop Dis. 2012:S442-5.
Rao KVN, Sunitha C, David B, Sandhya S, Shwetha D, Murali K. Diuretic activity on different extracts and formulation on aerial parts of Rumex vesicarius Linn. J Chem Pharm Res. 2011;3(6):400-8.
Dipsankar C, Preethi GP, Vinod N, Herur N, Sheetal U, Mirabel R. Diuretic activity of NR-ANX-C (a polyherbal formulation) in normal rats. J Pharm Res. 2010;3(5):956-9.
Rajesh M, Avijit C, Shyam P, Arun M. Evaluation of diuretic and laxative activity of methanolic extract of Sesbania grandiflora Pers. Dec J Pharmacol. 2010;1(1):21-8.
Noviany H, Hasnah O, Suriyati M, Wong KC, Khalijah A, Anis SMZ. The chemical components of Sesbania grandiflora root and their antituberculosis activity. J Pharma Sci. 2012;5:882-9.
Mukul B, Roop K, Chandru S. Preliminary phytochemical analysis of leaf and bark methanolic extract of Sesbania grandiflora. J Phytopharmacol. 2012;1(2):10-20.
Kakjing DF, Noel NW, Iliya HA, Chukwurah CJ. Diuretic activity of Ageratum conyzoides extract in rats. J Phytopharmacol. 2012;3(1):145-9.
Varadarassou MM, Subramanian K, Gnanapragasam S, Dhayalamurthi SDQ, Subramani K. Effect of Cansjera rheedii J. Gmelin (Opiliaceae) on diuretic activity in rats. J Pharma Res. 2009;2(10):1627-8.
Jesupillai M, Jasemine S, Palanivelu M. Diuretic activity of leaves of Erythrina indica Lam. Int J Green Pharm. 2010:218-9.
Suresh A, Senthil VS, Suresh V, Senthil KN, Phani KA. Evaluation of diuretic activity of Samanea saman (Jacq) merr bark in albino rats. J Glob Trends Pharm Sci. 2010;1(1):61-7.
Vyas BA, Vyas RB, Joshi SV, Shah PD, Santani DD. Effect of aqueous extract of Pergularia daemia on urine production. Der Pharmacia Lettre. 2011;3(4):207-14.
Vyas B, Vyas R, Santani DD. Diuretic potential of whole plant extracts of Pergularia daemia (Forsk.). Iran J Pharma Res. 2011;10(4):795-8.