Inhibition of free radical activity by dual PPAR α and PPAR γ agonist using analytical assay methods
Keywords:Diabetes mellitus, 1, 1 Diphenyl 2 Picryl hydrazide, Free radicals, Nitric oxide, Peroxisome proliferator receptor agonist, Saroglitzar
Background: Overproduction of free radicals involved in the pathology of a wide variety of clinical disorders. Poor glycaemic control in diabetic people leads to free radical production responsible for diabetic related complications. Antioxidants produces resistance against the oxidative stress by scavenging free radicals may useful in treating diabetic related complications. Saroglitazar is a newer antidiabetic drug act on dual Peroxisome Proliferator Receptor Agonist α (PPAR α) and PPAR γ agonist with protection effect on Diabetes mellitus induced lipid dystrophy. Our study was done to evaluate the in vitro antioxidant effect Saroglitazar by 1, 1 Diphenyl 2 Picryl hydrazide (DPPH) and Nitric Oxide (NO) method.
Methods: In this study, we demonstrated invitro antioxidant activity by using 10 mg/dl stock solutions of Saroglitazar. DPPH and NO free radical scavenging test were done for different concentration of Saroglitazar.
Results: Saroglitazar showed concentration dependent free radical scavenging activity in DPPH assay. In DPPH assay at higher concentration 1000ug concentration showed 49.18% free radical scavenging activity. At lower concentration 10ug showed 17.18% free radical scavenging activity. NO scavenging activity at lower concentration 100ug showed 55.15% activity. But the higher concentration (1000ug) only slight increase in 60.15% activity.
Conclusions: Thus Saroglitazar invitro antioxidant analysis proved that it is a potent antioxidant.
World Health Organization, Diabetes Fact Sheet 312. Available at: http://www.who.int/mediacentre/factsheets/fs312/en/index.html. Assessed on October 9, 2016.
Joshi SR. Management of Obese Indian Patient. Indian Journal of Obesity. 2005;1(1):11-20.
Narendhirakannan RT, Seema T. In vitro anti-oxidant studies on ethanolic extracts of leaves and stems of arbo-tristis L. International Journal of Biological and Medical Research. 2010;1(4):188-92.
Riley PA. Free radicals in biology: oxidative stress and effects of ionizing radiation. Int J Rad Biol. 1994;65:27-33.
Khalid R. Studies on free radicals, antioxidants and co-factors. Clinical Interventions in Aging. 2007;2(2):219-36.
Bajaj M, Suraamornkul S, Hardies LJ, Glass L, Musi N, DeFronzo R. Effects of peroxisome proliferator-activated receptor (PPAR)-α and PPAR-γ agonists on glucose and lipid metabolism in patients with type 2 diabetes mellitus. Diabetologia. 2007;50(8):1723-31.
Sharma A, Amarnath S, Kushwah DS, Ramaswamy S. Saroglitazar, a novel cardiometabolic agent for diabetic dyslipidemia: A Review. Journal of Young Pharmacists. 2015 Jan 1;7(1):2-6.
Yokozawa T, Chen CP, Dong E, Tanaka T, Nonaka GI, Nishioka I. Study on the inhibitory effect of tannins and flavonoids against the 1, 1-diphenyl-2-picrylhydrazyl radical. Biochemical pharmacology. 1998 Jul 15;56(2):213-22.
Alderton WK, Cooper CE, Knowles RG. Nitric oxide synthases: Structure, function and inhibition. Biochemical Journal. 2001 Aug 1;357(3):593-615.
Singh VP, Bali A, Singh N, Jaggi AS. Advanced glycation end products and Diabetic complications. Korrean J Physiol Pharmacol. 2014;18:1-14.
Gillery P. Advanced glycation end products (AGEs), free radicals and diabetes. J Soc Biol. 2001;195(4):387-90.
Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol. 2011;48(4):412-22.
Marinova G, Batchvarov V. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulg J Agric Sci. 2011;17(1):11-24.
Patel RM, Patel NJ. In vitro antioxidant activity of coumarin compounds by DPPH, super oxide and nitric oxide free radical scavenging methods. J Adv Pharm Educ Res. 2011;1:52-68.