Study of diuretic activity of aqueous extract of amaranthus spinosus linn on rats

Shravani Potllapalli, Jagannath Narumalla, Naga Teja Pavani A., Damodaram Govindadas, Somashekar S. Chikkannasetty


Background: Traditional Siddha medicine literature claims that the Amaranthus spinosus Linn. Whole plant possesses diuretic property. The aim of the present study is to investigate the diuretic activity of aqueous extract of amaranthus spinosus linn on rats.

Methods: The rats were randomly divided into four groups of six animals each and deprived of food for 18 hours before the experiments. Control (Group I) rats were treated with Normal saline (5ml/Kg,) and Standard (Group II) rats were administered furosemide 15mg/Kg, I.P. Group III and IV were treated with Aqueous extract of Amaranthus spinosus Linn (AEAS) 250 and 500 mg/kg in normal saline respectively, The urine was collected after 5 and 24 hours. The urine volume and the total concentration of Na+, K+, and Cl- in the urine were estimated. The data were presented as mean ± standard deviation and analyzed using one-way analysis of variance and Student's "t"-test. P <0.05 was considered statistically significant. Conventional windows software was used for statistical analysis.

Results: Urine analysis showed that Na+, K+, and Cl- levels were significantly (p<0.05) increased with AEAS 250 and 500 mg/kg as rats treated with furosemide 15mg/kg. Urine volume is significantly (p<0.05) increased in rats treated with drugs (AEAS 500 mg/kg and furosemide 15mg/kg) compared to normal saline 25ml/Kg, treated rats at 5 and 24 hours.

Conclusions: These data suggest that AEAS has produced notable diuretic effect which appeared to be comparable to that produced by the standard diuretic furosemide.


Amaranthus spinosus linn, Diuretic activity, Furosemide

Full Text:



Koti BC, Purnima A. Diuretic activity of extracts of Centratherumant helminticum. Int J Green Pharm. 2008;2:228-31.

Kore KJ, Jadhavpradip, Shete RV, Shetty SC. Diuretic activity of Tectona grandis leaves aqueous extract in wistar rats. IJPRD. 2011:3;141-46.

Upendrabhadoriya, Swetatiwary, Sharma P, Bankey S, Mourya M. Diuretic activity of extract of Salvia officinalis L. Asian Journal of Pharmacy and Life Science. 2011:1:24-8.

Mudhaliyar M. Medica SM. The Reliance Printers, India; 1998:342.

Varier PS. Indian Medicinal Plants. Orient Longman, India; 2005:121-124.

Khare CP. Indian medicinal plants illustrated dictionary. Springer published, New Delhi; 2014:41-42.

Azhar-ul-Haq, Malik A, Khan AU, Shah MR, Muhammad P. Spinoside, new coumaroyl flavone glycoside from Amaranthus spinosus. 2004;27:1216-9.

Buragohain J. Folk medicinal plants used in gynecological disorders in Tinsukia district, Assam, India. Fitoterapia. 2008;5:388-92.

Hilou A, Nacoulmaa OG, Guiguemdeb TR. In vivo antimalarial activities of extracts from Amaranthus spinosus L, Boerhaavia EL. in mice. Journal of Ethnopharmacology. 2006;103:236-40.

Ibewuike J, Ogundaini AO, Bohlin L, Ogungbamila FO. Anti-Inflammatory Activity of Selected Nigerian Medicinal Plants. J. Nat. Prod. And Med. 1997;1:10-4.

Cai Y, Sun M, Corke H. Antioxidant activity of betalains from plants of the Amaranthaceae. J Agric Food Chem. 2003;51:2288-94.

Patel R, Mahobia NK, Gendle R, Kauskik B, Singh SK. Diuretic activity of leaves of Plectranthus amboinicus (LOUR) Spreng in male albino rats. Phcog Res. 2010;2:86-8.

Jhade D, Ahirwar D, Jain R, Sharma N, Gupta S. Pharmacognostic standardization, physico and phytochemical evaluation of Amaranthus Spinosus linn. Root. J Young Pharm. 2011;3:221-5.

Hilou A, Nacoulma OG, Guiguemde TR. In vivo antimalarial activities of extracts from Amaranthus spinosus L. and Boerhaavi aerecta L. in mice. J Ethnopharmacol. 2006;103:236-40.

Naik ND, Kalugonda MK, Jayasri P, Elumalai A. Evulation of diuretic activity of Sidaspinosa Linn leaves extract. J. chem.pharm.res. 2011;6:1004-8.

Baral M, Chakraborty S, Chakraborty P. Evaluation of anthelmintic and anti-inflammatory activity of Amaranthus Spinosus Linn. Int J Curr Pharm Res. 2010;2(4):4447.

Yadav U, Hugo A, Boon EK. Indigenous use and bio-efficacy of medicinal plants in Rasuwa district, central Nepal. Journal of Ethano biology and Ethano medicine. 2010;6:3.