DOI: http://dx.doi.org/10.18203/2319-2003.ijbcp20172805

Anti asthmatic effect of Momordica Charantia and its comparison with montelukast an in vitro and in vivo model

Bhuvaneswari K., Swarna R. M. P. L., Amudhan Aravind

Abstract


Background: Momordica charantia (MC) (bitter gourd) have shown the inhibition of NF-κβ and Leukotrienes expression in many inflammatory pathological conditions. Based on ist anti-inflammatory action this study aimed to Identify MC fruit dry powder (MCp) and MC fresh juice (MCj) action on airway inflammation in Guinea Pig model and also to find out the presence of alkaloids and flavonoides.

Methods: 18 adult Guinea pigs of both sexes (excluding mating animals) were randomly divided into three groups with six animals in each namely Montelukast, MCj and MCp groups respectively. They were given with OD oral administration of concerned drugs for 1 -7 days. 6 control animals were exposed to 2% histamine aerosol for 120 seconds using histamine chamber prior to treatment with Montelukast, MCj and MCp to observe the normal Pre-Convulsive Dyspnea (PCD) in seconds. After six hours of daily OD oral dose of test drugs administration, all the groups’ were exposed to 2% Histamine aerosol one by one for 120 sec on day1 & 7 to observe PCD. The MCj and MCp were also tested with chemical assay, TLC to confirm the presence of alkaloids, flavonoides.

Results: ON DAY 1 and 7: MCj and MCp showed significant decrease in PCD occurrence and it is statistically significant compared to the control. PCD blocking action of MCj group was effective in DAY 1. TLC and Chemical Assay were not supportive for bronchodilator action.

Conclusions: MCj and MCp have promising preventive role in asthma. This study had expressed the positive protective role of Momordica charantia in asthmatic condition based on its known anti-inflammatory action.

 


Keywords


Guinea pig, Momordica charantia, Pre-convulsive dyspnea

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