Anti asthmatic effect of Momordica Charantia and its comparison with montelukast an in vitro and in vivo model
Keywords:Guinea pig, Momordica charantia, Pre-convulsive dyspnea
Background: Momordica charantia (MC) (bitter gourd) have shown the inhibition of NF-κβ and Leukotrienes expression in many inflammatory pathological conditions. Based on ist anti-inflammatory action this study aimed to Identify MC fruit dry powder (MCp) and MC fresh juice (MCj) action on airway inflammation in Guinea Pig model and also to find out the presence of alkaloids and flavonoides.
Methods: 18 adult Guinea pigs of both sexes (excluding mating animals) were randomly divided into three groups with six animals in each namely Montelukast, MCj and MCp groups respectively. They were given with OD oral administration of concerned drugs for 1 -7 days. 6 control animals were exposed to 2% histamine aerosol for 120 seconds using histamine chamber prior to treatment with Montelukast, MCj and MCp to observe the normal Pre-Convulsive Dyspnea (PCD) in seconds. After six hours of daily OD oral dose of test drugs administration, all the groups’ were exposed to 2% Histamine aerosol one by one for 120 sec on day1 & 7 to observe PCD. The MCj and MCp were also tested with chemical assay, TLC to confirm the presence of alkaloids, flavonoides.
Results: ON DAY 1 and 7: MCj and MCp showed significant decrease in PCD occurrence and it is statistically significant compared to the control. PCD blocking action of MCj group was effective in DAY 1. TLC and Chemical Assay were not supportive for bronchodilator action.
Conclusions: MCj and MCp have promising preventive role in asthma. This study had expressed the positive protective role of Momordica charantia in asthmatic condition based on its known anti-inflammatory action.
Global Initiative for Asthma. Global stargery for asthma management and prevention, 2017. Available from: www.ginasthma.org [Accessed 1st May 2017]
Pharmacological actions and potential uses of Momordica charantia: a review 9 jEthnopharmacol. 2004;93(1):123-32.
Kumar SKP, Bhowmik D. Traditional Medicinal Uses and Therapeutic Benefits of Momordica Charantia Linn. International Journal of Pharmaceutical Sciences Review and Research. 2010;4(3):23-8.
Mariko M, Ryo T, Teruko N. Induction of Anti-inflammatory Responses by Dietary Momordica charantia L. (Bitter Gourd) Biosci. Biotechnol. Biochem. 2003;67(12):2512-7.
Nguyen, Pham, Nguyen, Tran, Phan, Chau. Inhibition of Nuclear Transcription Factor-jBand Activation of Peroxisome Proliferator-Activated Receptors in HepG2 Cells by Cucurbitane-Type Triterpene Glycosides from Momordica Charantia. J Med Food. 2012;15(4):369-77.
Chao C, Sung P, Wang W, Kuo Y. Anti-Inflammatory Effect of Momordica Charantia In Sepsis Mice. Molecules. 2014;19:12777-88.
Sridevi G, Gopkumar P, Ashok S, Shastry C. Pharmacological Basis For Antianaphylactic, Antihistaminic and Mast Cell Stabilization Activity of Ocimum Sanctum. The Internet J Pharmacol. 2009;7(1).
Singh U, Sagar VR. Effect of drying methods on nutritional composition of dehydrated bitter gourd (Momordica Charantia.). Indian Agricultural Research Institute, New Delhi-12. Agriculture for Sustainable Development. 2013;1(1):83-6.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Internationale pharmaceutica sciencia. 2011 Mar;1(1):98-106.
Nagy G, Szöllősi I, Szendrei K. Colour Tests for Precursor Chemicals of Amphetamine-Type substancesthe Use of Colour Tests for Distinguishing between Ephedrine-Derivatives un scientific and technical notes. Scitec; 2005:16-17.
Abalaka ME, Inabo HI, Onaolapo JA, Olonitola OS. Chromatographic Analysis and Antibacterial Effects of the Ethanolic Extracts of Momordica Charantia. Afr J Med Phy, Biomed Eng and Sc. 2010;2:14-20.
Tsai CH, Chen EC, Tsay HS, Huang CJ. Wild bitter gourdimproves metabolic syndrome: a preliminary dietary supplementationtrial. Nutr J. 2012;11:4.
Klomann SD, Mueller AS, Pallauf J, Krawinkel MB. Antidiabeticeffects of bitter gourd extracts in insulin-resistant db/db mice. BrJ Nutr. 2010;104:1613-20.
Virdi J, Sivakami S, Shahani S, Suthar AC, Banavalikar MM, Biyani MK. Antihyperglycemic effects of three extracts from Momordica Charantia. J Ethnopharmacol. 2003;88:107-11.
Fuangchan A, Seubnukarn T, Jungpattanawadee D, Sonthisombat P, Ingkaninan K, Plianbangchang P, et al. Retrospective study on the use of bitter melon for type 2 diabetes at Dansai Crown Prince Hospital, Thailand. Srinagarind Med J. 2009;24:332-8.