Comparative study of hypoglycaemic activity of morus alba with oral hypoglycaemic drug (metformin) in alloxan induced diabetic rats

Naveen Kumar Madalageri, Lavanya Nagaraj


Background: Diabetes mellitus is a spectrum of common metabolic disorders, arising from a variety of pathogenic mechanisms, all resulting in hyperglycemia. The number of individuals with diabetes is rising rapidly throughout the world. Worldwide the prevalence of diabetes mellitus is estimated to be 2.8% and is set to rise to 4.4% by 2030. In India alone, the prevalence of diabetes is expected to increase from 30.9 million to 69.9 million by 2025. Despite the availability of many drugs for treatment for diabetes and its complications continue to be major medical problems. Currently available antidiabetic drugs are not completely effective and are associated with adverse effects both in the short and long run. Some studies have reported many different medicinal properties of mulberry leaves (Morus alba) like neuroprotective property, cardioprotective property, antidepressant property. It is used as anti-inflammatory, diuretic, expectorant and antidiabetic in traditional Chinese medicine. So this study is undertaken to evaluate the hypoglycaemic effect of aqueous extract of leaves of Morus alba in Alloxan induced diabetic albino rats.

Methods: Total 24 albino rats of either sex weighing 150-200 gms were selected and randomly divided into 4 equal groups containing 6 rats in each group. First group (Non-diabetic control) was given normal saline (0.5 mL). Other 3 groups were induced diabetes by giving Alloxan (150 mg/kg IP). They were subdivided into diabetic control group receiving normal saline (0.5 mL), standard control group receiving Metformin (10 mg/kg body weight) and test group receiving Morus alba extract (600 mg/kg). Fasting blood sugar level was estimated on day 0, 1, 3, 7, 14, 21 and 28 by using glucometer. Data were statistically analysed by Tukey’s Post Hoc test and ANOVA.

Results: Morus alba at the above doses significantly reduced blood glucose levels as compared to control group (p<0.01), but as compared to standard drugs (Metformin) it is statistically insignificant (p>0.05).

Conclusions: The result suggests that Morus alba leaves have hypoglycaemic activity.


Albino rats, Alloxan, Hypoglycaemia, Metformin, Morus alba

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Alvin C. Powers, David D’Alessio, Endocrine Pancreas and Pharmacotherapy of Diabetes Mellitus and Hypoglycemia. The pharmacological basis of therapeutics. Goodman and Gilman.12th ed. Mc Graw-Hill; 2011:1243.

Park K. Diabetes mellitus. Parks textbook of preventive and social medicine. 18th edi. Jabalpur: M/S Banarsidas Bhanot Publishers; 2005:312.

Madalageri NK, Nagaraj L, Nidamarthi SB. Evaluation and comparative study of hypoglycaemic activity of morus alba with oral hypoglycaemic drug (glibenclamide) in alloxan induced diabetic rats. J. Evolution Med. Dent. Sci. 2016;5(48):3062-5.

Raheja BS, Kapor A, Bhaskar A, Sathe SR, Jorgensen LN, Moorthi SR. Diabetes care in India-current studies. JAPI. 2001;49(7):712-22.

Mohammadi J, Naik PR. Evaluation of hypoglycemic effect of Morus alba in an animal model. Indian J Pharmacology. 2008;40(1):15-8.

Padwal R, Varney J, Majumdar AR, McAlistar FA, JA Johnson. Diabetes Care. 2005;28(3):736-44.

Benny, Abraham K, Adithan C. Indian J Pharmacol. 2000;32:S67-80.

Yeh GY, Kaptchuk JJ, DM Eisenberg, Phillips RS. Diabetes Care. 2003;26:1277-94.

Kang TH. Enhancement of Neuroprotection of Mulberry leaves (Morus alba L) prepared by the Anaerobic treatment against ischemic damage. Biol Pharm Bull. 2006;29:270-4.

Enkhmaa. Mulberry (Morus alba L) leaves and major flavonal Quercetin 3-(6-malonylglucaside) attenuate Atherosclerotic Lesion development in LDL receptor- deficient mice. J Nutr. 2005;135:729-34.

Sattayasi J, Tiamkao S, Puapairoj P. Biphasic effect of Morus alba Leaves (Green Tea) Extracton Micein Chronic Forced Swimming Model. Phytother. Res. 2008;22:487-92.

Andallu B, Suryakantham V, Lakshmi B, Reddy GK. Effect of mulberry ( Morus indica L.) therapy on plasma and erythrocyte membrane lipids in patients with type 2 diabetes. Clin Chim Acta. 2001;314:47-53.

Andallu B, Varadacharyulu N. Control of hyperglycemia and retardation of cataract by mulberry (Morus indica L.) leaves in streptozotocin diabetic rats. Indian J Exp Biol. 2002;40:791-5.

Andallu B, Varadacharyulu N. Antioxidant role of mulberry (Morus indica Anantha) leaves in streptozotocin diabetic rats. Clin Chim Acta. 2003;338:3-10.

Saeed arayne M. In vitro hypoglycemic activity of methanolic extract of some indigenous plants, Pak. J. Pharm Sci. 2007;20(4):261-8.

Nickavara B, Mosazadeha G. Influence of Three Morus Species Extracts on α-Amylase Activity. Iranian Journal of Pharmaceutical Research. 2009;8(2):115-9.