Preclinical HbA1c level studies of makaradhwaja and siddha makaradhwaja after chronic administration to male Sprague-Dawley rats

Arjyabrata Sarker, Kushal Biswas, Manjurul I. Chowdhury, Farzana Khan, Taposhi Sultana, Farjana Afrin, Md. Afaz Uddin, Md. Al Foyjul Islam, M.S.K. Choudhuri


Background: Makaradhwaja (MD) and Siddha Makaradhwaja (SMD) are Ayurvedic preparations used as traditional medicines for different clinical indications in the rural population. Principle purpose of using MD is controlling hypotension and while SMD is useful in peripheral circulatory failure treatment. In this study we evaluate the influence of these preparations on HbA1c (%) level.

Methods: To find out the average plasma glucose concentration over prolonged period of time, MD and SMD were administered chronically to the male Sprague-Dawley rats at a dose of 40 mg/kg. After 28 days of chronic administration of MD and SMD the following changes were noted and Glycated Hemoglobin (HbA1c) level was determined.

Results: The results of the study of in vitro quantitative determination of rat HbA1c concentrations in serum studies, MD demonstrated a negligible (0.61%) decrease in the HbA1c level of the blood of the male rat (p=0.902). Whereas SMD demonstrated a negligible (1.83%) increase in the HbA1c level of the blood of the male rat (p=0.782).

Conclusions: Between these preparation MD slightly decreases HbA1c level of the blood of the male rat, whereas SMD found in increasing HbA1c level of the blood of the male rat.


Diabetes mellitus, HbA1c, Makaradhwaja, Siddha Makaradhwaja

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