Lipid lowering and antioxidant potential of Asparagus racemosus in hyperlipidemic rats

Rama R. Bhosale, Jugalkishor B. Jaju, Sudhir L. Padwal, Rakesh R. Jadhav, Vinod S. Deshmukh


Background: Atherosclerosis is associated with hyperlipidemia which is a major risk factor for coronary artery disease. Therefore, treatment of hyperlipidemia is one of the major approaches to decrease the atherogenic process. Many studies revealed that Asparagus racemosus (AR) possesses hypolipidemic and antioxidant potential, but results were not consistent. Therefore, the present study was undertaken to investigate lipid lowering and antioxidant potential of AR root powder in hyperlipidemic rats.

Methods: Hyperlipidemia was induced in normal rats by including 0.75 gm% cholesterol and 1.5 gm% bile salt in normal diet and these rats were used for the experiments. Dried root powder of Asparagus racemosus was administered as feed supplement at 5 gm% and 10 gm% dose levels to the hyperlipidemic rats. Plasma lipid profile, malondialdehyde, ascorbic acid, catalase and superoxide dismutase were estimated using standard methods. Statistical analysis was done by one way analysis of variance (ANOVA).

Results: Feed supplementation with 5 gm% and 10 gm% Asparagus racemosus resulted in a significant decrease in plasma cholesterol, LDL and significant increase in HDL. But there were no significant decrease in triglycerides and VLDL. The feed supplementation increased activities of catalase, superoxide dismutase and ascorbic acid content increased significantly in both the experimental groups (5 and 10 gm% supplemented groups). But there was no significant change in the concentration of malondialdehyde in these groups.

Conclusions: The present study demonstrated that addition of Asparagus racemosus root powder at 5 gm% and 10 gm% level as feed supplement reduces the plasma lipid levels and also acts as an antioxidant.


Antioxidant, Asparagus racemosus, Hyperlipidemia, Lipid profile

Full Text:



Ajayi OB, Ajayi DD. Effect of oilseed diets on plasma lipid profile in albino rats. Pak J Nutr 2009;8:116-8.

Kothari S, Jain AK, Mehta SC, Tonpay SD. Effect of fresh Triticum aestivum grass juice on lipid profile of normal rats. Indian J Pharmacol 2008;40:235-6.

Sandeep S, Koneri R, Mushirbhai MI. Evaluation of antihyperlipidimic activity of saponin fraction of roots of Memordica cymbalaria. Indian J Pharmacol 2008;40 (Suppl 2):S221–S236.

Sundaram R, Mitra SK. Antioxidant activity of ethyl acetate soluble fraction of Acacia arabica bark in rats. Indian J Pharmacol 2007;39:33-8.

Lindequist U, Niedermeyer THJ, Julich WD. The pharmacological potential of mushrooms. Evid Based Complement Alternat Med 2005;2:285-99.

Nozaki K, Hikiami H, Goto H, Nakagawa T, Shibahara N, Shimada Y. Keishibukuryogan (Gui-Zhi-Fu-Ling-Wan), a kampo formula, decreases disease activity and soluble vascular adhesion molecule-1 in patients with rheumatoid arthritis. Evid Based Complement Alternat Med 2006;3:359-64.

Venkatesan N, Devaraj SN, Devaraj H. Increased binding of LDL and VLDL to apo B,E receptors of hepatic plasma membrane of rats treated with fibernat. Eur J Nutr 2003;42:262-71.

Venkatesan N, Thiyagarajan V, Narayanan S, Arul A, Raja S, et al. Anti-diarrhoeal potential of Asparagus racemosus wild root extracts in laboratory animals. J Pharm Pharm Sci 2005;8:39-46.

Bopana N, Saxena S. Asparagus racemosus--ethno pharmacological evaluation and conservation needs. J Ethnopharmacol 2007;110:1-15.

Visavadiya NP, Narsimhacharya AVRL. Asparagus racemosus regulates Cholesterol metabolism and Improves antioxidant status in Hyperlipidemic rats. Evid Based Complement Alternat Med 2009;6:219-26.

Visavadiya NP, Narsimhacharya AV. Hypolipidemic and antioxidant activities of Asparagus racemosus in Hyperlipidemic rats. Indian J Pharmacol 2005;37:376-80.

Roeschlau P, Bernt E, Gruber W. Enzymatic determination of total cholesterol in serum. Z Klin Chem Klin Biochem 1974;12:226.

McGowan MW, Artiss JD, Strandbergh DR, Zak B. A peroxidase-coupled method for the colorimetric determination of serum triglycerides. Clin Chem 1983;29:538-42.

Lorentz K, Flatter B, Augustin E. Arylesterase in serum: Elaboration and clinical application of a fixed incubation period. Clin Chem 1979;25:1714-20.

Chatterjee MN. Textbook of Medical Biochemistry, 7th edition. JAYPEE Brothers: New Deli, India 2007;418-20.

Schaffert RR, Kingsley GR. A rapid, simple method for the determination of reduced, dehydro-, and total ascorbic acid in biological material. J Biol Chem1955;212:59-68.

Cohen G, Dembiec D, Marcus J. Measurement of catalase activity in tissue extracts. Anal Biochem1970;34:30-8.

Pasha KV, Sadasivadu B. Intracellular content of thiol compounds, thiobarbituric acid reactive substances and gamma-glutamyl transpeptidase in rat brain during anoxia. Neurosci Lett 1984;46:209-14.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.

Mahajan BK. Significance of difference in means. In: Mahajan BK (ed) Methods in biostatistics for medical and research workers. 6th ed. New Delhi: JAYPEE Brothers Medical Publishers, 2006:130-55.

Ikeda I, Sugano M. Inhibition of cholesterol absorption by plant sterols for mass intervention. Curr Opin Lipidol 1998;9:527-31.

Quilez J, Garcia-Lorda P, Salas-Salvado J. Potential uses and benefits of phytosterols in diet: present situation and future directions. Clin Nutr 2003;22:343-51.

Harwood HJ Jr, Chandler CE, Pellarin LD, Bangerter FW, Wilkins RW, Long CA, et al. Pharmacologic consequences of cholesterol absorption inhibition: alteration in cholesterol metabolism and reduction in plasma cholesterol concentration induced by the synthetic saponin β-tigogenin cellobioside (CP-88818; tiqueside). J Lipid Res 1993;34:377-95.

Oakenfull DG, Sidhu GS. Could saponins be a useful treatment for hypercholesterolaemia? Eur J Clin Nutr 1990;44:79-88.

Assmann G, Nofer J. Atheroprotective effects of high density lipoproteins. Annu Rev Med 2003;54:321-41.

Young IS, Woodside JV. Antioxidants in health and diseases. J Clin Pathol 2001;54:176-86.

Block G, Dietrich M, Norkus EP, Morrow JD, Hudes M, Caan B, Packer L. Factors sssociated with oxidative stress in human populations. Am J Epidemiol 2002;156:274-85.

Bagchi K. Nutrition in the Eastern Mediterranean Region of the World Health Organization. East Mediterr Health J 2008;14 Suppl:S107-13.

Tiwari AK. Imbalance in antioxidant defence and human diseases: multiple approach of natural antioxidants therapy. Curr Sci 2001;81:1179-80.

Barter P, Gotto AM, LaRosa JC, Maroni J, Szarek M, Grundy SM, Kastelein JJ, et al. HDL cholesterol, very low levels of LDL cholesterol, and cardiovascular events. N Engl J Med 2007;357:1301-10.

Ramakrishnan V, Gopala Reddy A, Kalakumar B. Antioxidant actions of Asparagus racemosus against iron induced oxidative stress in broilers. Indian J Pharmacol 2008;40(Suppl 2):S160-S171.

Visavadiya NP, Soni B, Soni B, Madamwar D. Suppression of reactive oxygen species and nitric oxide by Asparagus racemosus root extract using in vitro studies. Cell Mol Biol (Noisy-le-grand) 2009;55 Suppl:OL1083-95.

Muralidharan P, Nandagopal M. Cerebroprotective effect of methanolic extract of Asparagus racemosus in cerebral ischemia induced by bilateral carotid artery occlusion in rats. Indian J Pharmacol 2008;40(Suppl 2):S112-S142.

Wiboonpun N, Phuwapraisirisan P, Tip-pyang S. Identification of antioxidant compound from Asparagus racemosus. Phytother Res 2004;18:771-3.

Parihar MS, Hemnani T. Experimental excitotoxicity provokes oxidative damage in mice brain and attenuation by extract of Asparagus racemosus. J Neural Transm 2004;111:1-12.

Kamat JP, Boloor KK, Devasagayam TP, Venkatachalam SR. Antioxidant properties of Asparagus racemosus against damage induced by gamma-radiation in rat liver mitochondria. J Ethnopharmacol 2000;71:425-35.

Bhatnagar M, Sisodiya SS, Bhatnagar R. Antiulcer and antioxidant Activity of Asparagus racemosus Willd and Withania somnifera Dunal in rats. Ann N Y Acad Sci 2005;1056:261-78.