Analgesic and antipyretic activity of methanolic extract of Leucas Clarki in animal models

Truptirekha Swain, Rashmita Pradhan, Debasish Barik

Abstract


Background: Search for an indigenous safe and effective analgesic and antipyretic agent continues to be an exciting area of research. The objective of this study was to evaluate the analgesic and antipyretic activity of methanolic extract of Leucas clarki, in animal models.

Methods: Analgesic activity was evaluated in albino mice by acetic acid induced writhing test. Antipyretic study was carried out in albino rats by inducing pyrexia with brewer’s yeast. Analgesic activity was expressed as percentage of reduction in number of writhings compared to vehicle (Tween-80) and antipyretic activity was expressed as reduction of rectal temperature compared to basal temperature, after 18 hours of induction of pyrexia. Leucas clarki extract was administered at 3 doses (100,200,400mg/Kg) intraperitoneally. Student’s paired and unpaired “t” test was applied to test significance.

Results: Methanolic extract of Leucas clarki (MLC) at dose 200 mg and 400mg /kg produced 19.17% & 32.03% of reduction of no. of writhing induced by acetic acid compared to that of Tween 80 treated control mice, However this is less compared to aspirin treated control rats. In model of pyrexia all the three doses of MLC produced significant reduction of rectal temperature compared to basal temperature (18hrs). Antipyretic effect was appreciated at very first hour and persisted till the end of 6 hours like that of paracetamol. Both 100 &200 mg/kg MLC were equivalent in potency as that Paracetamol (33mg/kg I.P) and 400mg/kg was even better than Paracetamol.

Conclusions: Methanolic extract of Leucas clarki possesses excellent antipyretic and analgesic property.


Keywords


Leucas Clarki, Analgesic activity, Antipyretic activity

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References


Unwanted effects and adverse drug reactions, in Clinical Pharmacology, Bennett & Brown, 9th Edition, Page 139-140.

V. Tandor, B. Kapoor. Herbal drug research in India: A trend analgesic using IJP as a marker (1995-2003). Indian J Pharmacol 2004;6:99-100.

Monograph on Indian Lucas R. Br.(Dronapushpi) Lamiaceae by V.Singh; Scientific Publishers (India), Jodhpur, ISBN: 81- 7233-286-6.

Kakalisaha, Pulak k. Mukharjee, J. Das, Subasch. Das, Manal M. Hypoglycemic activity of Lucas lavandilofulia Rees in streptozotocin induced diabetic rats. Phytotherapy Research;6:373-376.

S. Sahu, Swain TR, A. Shree, S.N Pandeya. Different phytochemical analysis and antidiabetic activity of leucas clarki. Dissertation submitted to the BijuPattanaik university of technology (BPUT) in partial fulfilment for the award of the degree of masters of pharmacy in pharmaceutical chemistry.

Some common techniques. Screening of analgesic activity of different compound, Fundamentals of experimental pharmacology, M.N. Ghosh: 3rd edition. page 176.

Loux JJ, Depalma PD, Yarkell SL. Antipyretic testing of aspirin in rats. Toxicol Appl Pharmcol 1972;22:672-5.

Hossinzadeh H, Ramezini M, Fadishei M. Antinociceptive, anti inflammatory and acute toxicity effects of Zhumeriamajdac extracts in mice and rats. Phytomediam 2002;9:135-41.

M. Bishnoi, C.S. Patil, A. Kunal et al. Analgesic activity of acetyl-11-Keta-beta-boswellic acid, a 5-lipoxygense inhibitor. Indian J Pharmacol. 2005;37:4.

Anti inflammatory and antinociceptive activaties of Zingiberofficinale roscoe Essential oil in experimental animal models. Indian J Pharmacol 2006;38:58-59.

Suekawa M, Ishige A, Yuasa K. Pharmacological studies on ginger. 1. Pharmacological actions of purgent constituents, (6)-gingerol and (6) shogaol. J Pharmacobiodyn 1984;7:836-48.