An experimental study of a novel combination of a herbal drug with an allopathic drug to evaluate the antihyperglycemic effect of irbesartan plus curcumin and comparison with glibenclamide

Sharad Tularam Leve, Himani Banga, Pratap Shankar, Rakesh Kumar Dixit

Abstract


Background: Curcumin has been used as a traditional remedy for many diseases in Ayurveda. Many studies have proved antidiabetic and antihyperlipidimic effects of curcumin. The present study was conducted to evaluate antihyperglycemic actions of Irbesartan in combination with Curcumin.

Methods: After baseline sampling, 18 rats of diabetic groups i.e. Group 1 to Group 3, were started with exclusive high fat diet and water ad libitum for next 4 weeks. At the end of 4 weeks, each rat was given Inj. Streptozotocin in a dose of 30 mg/kg body weight, dissolved in 0.2 ml of citrate buffer (pH 4.5) intraperitoneally. Drugs were given orally in normal saline by gastric gavage with the help of rat feeding cannula.

Results: Rats of diabetic group 2, receiving combination of Irbesartan 20mg/kg and Curcumin 200mg/kg body weight for 6 weeks, have shown significant decrease is mean FPG level (FPG- 29.69% reduction, p<0.001). Irbesartan+Curcumin had no any hypoglycemic effect in normoglycemic rats as well as diabetic rats.

Conclusions: Irbesartan in combination with Curcumin has significant antihyperglycemic effects. At the same time, Irbesartan combined with Curcumin, does not produce hypoglycemia in normal as well as diabetic rats.


Keywords


Curcumin, Irbesartan, Glibenclamide, Antihyperglycemic, Diabetes mellitus

Full Text:

PDF

References


Powers AC, D’Alessio D. Endocrine Pancreas and Pharmacotherapy of Diabetes Mellitus and Hypoglycemia. In: Goodman & Gilman’s The Pharmacological Basis of Therapeutics. 12th ed., Brunton L, Chabner B, Knollman BB, editors. Mc. Graw Hill, New York, 2011:1237-73.

Gerstein HC, Miller ME, Byington RP, Goff DC, Jr., Bigger JT, Buse JB, et al. (2008). Effects of intensive glucose lowering in type 2 diabetes. N Engl J Med 2008;358:2545-59.

Fasinu PS, Bouic PJ, Bernd R. An overview of the evidence and mechanisms of herb-drug interactions. Front Pharmacol 2012;3:69.

Kessler RC, Davis RB, Foster DF, Van Rompay MI, Walters EE, Wilkey SA, Kaptchuk TJ, Eisenberg DM. Long-term trends in the use of complementary and alternative medical therapies in the United States. Ann Intern Med 2001;135:262-8.

Mok DKW, Chau F. Chemical information of Chinese medicines: a challenge to chemist. Chemometrics Intell Lab Syst 2006;82:210-7.

Wang JF, Wei DQ, Chou KC. Drug candidates from traditional Chinese medicines. Curr Top Med Chem 2008;8:1656-65.

Wills RB, Bone K, Morgan M. Herbal products: active constituents, modes of action and quality control. Nutr Res Rev 2000;13:47-77.

Nurullahoğlu-Atalik KE, Okudan N, Belviranli M, Gökbel H, Simşek L. Curcumin increases vasodilatory effect of cilostazol in diabetic rat aorta. Indian J Exp Biol 2012;50:128-32.

Gupta R, Gupta RS. Effect of pterocarpus marsupium in Streptozotocin-induced hyperglycemic state in rats: comparison with glibenclamide. Diabetol Croat 2009;38:39-45.

Wang HJ, Jin YX, Shen W, Neng J, Wu T, Li YJ, Fu ZW. Low dose streptozotocin (STZ) combined with high energy intake can effectively induce type 2 diabetes through altering the related gene expression. Asia Pac J Clin Nutr 2007;16 Suppl 1:412-7.

Mohammed A, Adelaiye AB, Abubakar MS, Abdurahman EM. Effects of aqueous extract of Ganoderma lucidum on blood glucose levels of normoglycemic and alloxan induced diabetic wistar rats. J Medicinal Plants Res 2007;1:34-7.

Paget GE, Barnes JM. Evaluation of Drug Activities. In: Pharmacometrics, Lawrence DR, Bacharach AL, Eds., Vol. 1. Academic Press: New York; 1964.

Robertson RP, Harmon J, Tran PO, Tanaka Y, Takahashi H. Glucose toxicity in beta-cells: type 2 diabetes, good radicals gone bad, and the glutathione connection. Diabetes 2003;52:581-7.

Robertson RP, Harmon J, Tran PO, Poitout V. Beta-cell glucose toxicity, lipotoxicity and chronic oxidative stress in type 2 diabetes. Diabetes 2004;53 (suppl 1):S119-24.

Janson J, Ashley RH, Harrison D, McIntyre S, Butler PC. The mechanism of islet amyloid polypeptide toxicity is membrane disruption by intermediate-sized toxic amyloid particles. Diabetes 1999;48:491-8.

Ghaisas MM, Malusare PV, Talele SG, Kshirsagar AD, Deshpande AD. Hypoglycemic And Anti-Hyperglycemic Study Of Combination Of Curcumin and Quercetin In Normoglycemic Rats. Inventi Impact: Planta Activa 2011; Article ID- "Inventi:ppa/49/11", 2011.

Nolte MS. Pancreatic Hormones & Antidiabetic Drugs. In: Basic and Clinical Pharmacology 11th ed., Katzung BG, Masters SB, Trevor AJ editors. Tata McGraw Hill Education private Limited: New Delhi; 2009:727-51.

Sharma HL, Sharma KK. Insulin, Oral Hypoglycemics and Other related agents. In: Principles of Pharmacology, 1st ed. Paras Medical Publishers: Hyderabad; 2007:642-56.

Sharma RK, Patki PS. Double-blind, placebo-controlled clinical evaluation of an Ayurvedic formulation (Gluco Care capsules) in non-insulin dependent diabetes mellitus. Journal of Ayurveda & Integrative Medicine 2010;1:45-51.

Tripathi KD. Insulin, Oral Hypoglycemic Drugs and Glucagon. In: Essentials of Medical Pharmacology 6th ed. Jaypee Brothers Medical Publishers (P) Ltd.: New Delhi; 2008:254-74.